비평면 형상의 고체 지지체를 이용하여 미생물로부터핵산을 분리하는 방법,상기 분리된 핵산을 주형으로 하여핵산을 증폭하는 방법 및 상기 고체 지지체를 포함하는핵산 분리 및 증폭 장치
    62.
    发明公开
    비평면 형상의 고체 지지체를 이용하여 미생물로부터핵산을 분리하는 방법,상기 분리된 핵산을 주형으로 하여핵산을 증폭하는 방법 및 상기 고체 지지체를 포함하는핵산 분리 및 증폭 장치 失效
    使用非固体基质从微生物细胞中分离DNA的方法,使用分离的核酸作为模板来放大核酸的方法和用于分离和放大包含非固体基质的核酸的装置

    公开(公告)号:KR1020080030997A

    公开(公告)日:2008-04-07

    申请号:KR1020080025915

    申请日:2008-03-20

    CPC classification number: C12Q1/6844 C12Q1/686

    Abstract: A method for isolating a DNA from a microorganism cell is provided to perform effectively microorganism isolation and nucleic acid isolation simultaneously, simplify the isolation process separate nucleic acids without use of additional nucleic acid isolating agent such as chaotropic agent, and perform the isolation by using small devices such as a lap-on-a-chip. A method for isolating a DNA from a microorgansim cell by using a nonplanar solid substrate comprises the steps of: contacting the nonplanar solid substrate with a sample containing microorganisms including bacteria, fungi or viruses at pH 3.0-6.0 to adhere the microorganisms to the solid substrate; removing unadhered materials by washing the solid substrate; and crushing the microorganisms adhered to the solid substrate at pH 3.0-8.0 or pH 11-14, wherein the sample is blood, urine or saliva.

    Abstract translation: 提供了从微生物细胞中分离DNA的方法,可以同时有效地进行微生物分离和核酸分离,简化独立核酸的分离过程,而不使用额外的核酸分离剂如离液剂,并使用小分子 诸如芯片上的设备。 通过使用非平面固体底物从微生物细胞中分离DNA的方法包括以下步骤:使pH值为3.0-6.0的非平面固体基质与含有细菌,真菌或病毒的微生物的样品接触,以将微生物粘附到固体底物 ; 通过洗涤固体基底去除未粘附的材料; 并在pH3.0-8.0或pH11-14下破碎附着在固体基质上的微生物,其中样品是血液,尿液或唾液。

    비평면 형상의 고체 지지체를 이용하여 미생물로부터핵산을 분리하는 방법,상기 분리된 핵산을 주형으로 하여핵산을 증폭하는 방법 및 상기 고체 지지체를 포함하는핵산 분리 및 증폭 장치
    63.
    发明公开
    비평면 형상의 고체 지지체를 이용하여 미생물로부터핵산을 분리하는 방법,상기 분리된 핵산을 주형으로 하여핵산을 증폭하는 방법 및 상기 고체 지지체를 포함하는핵산 분리 및 증폭 장치 有权
    使用非固体基质从微生物细胞中分离DNA的方法,使用分离的核酸作为模板来放大核酸的方法和用于分离和放大包含非固体基质的核酸的装置

    公开(公告)号:KR1020080017209A

    公开(公告)日:2008-02-26

    申请号:KR1020060079056

    申请日:2006-08-21

    CPC classification number: C12Q1/6834 C12Q1/6806 C12Q1/6844

    Abstract: A method for separating a nucleic acid from a microorganism is provided to perform simultaneously and efficiently a microorganism separation and a nucleic acid separation without using a separate nucleic acid separation agent such as a chaotropic agent. And a device for separating and amplifying a nucleic acid is provided to perform a separation and an amplification of the nucleic acid in same or different container. A method for separating a microorganism such as bacteria, fungi and virus comprises the steps of: (a) contacting a non-planar solid substrate with a sample including the microorganism such as blood, urine or saliva under pH of 3.0-6.0 to attach the microorganism to the solid substrate; and (b) lysing the microorganism attached to the solid substrate under the pH of 3.0-8.0, wherein the solid substrate is selected from the group consisting of a solid substrate having a pillar structure where a plurality of pillar are formed, a bead-shaped solid substrate and a sieve-structured solid substrate, has a water contact angle of 70-95° and includes at least one amine-based functional group on the surface thereof. After the contacting step, materials not attached to the solid substrate and other than the target microorganism are washed. A device for separating and amplifying a nucleic acid comprises a chamber including a non-planar solid substrate, a heating portion for heating the chamber, and a temperature controlling portion for controlling the heating of the chamber.

    Abstract translation: 提供了从微生物中分离核酸的方法,以不使用分离剂等分离的核酸分离剂,同时且有效地进行微生物分离和核酸分离。 提供用于分离和扩增核酸的装置,以在相同或不同的容器中进行核酸的分离和扩增。 用于分离微生物如细菌,真菌和病毒的方法包括以下步骤:(a)使非平面固体基质与包含微生物例如血液,尿液或唾液的样品接触,pH = 3.0-6.0,以将 微生物固体基质; 和(b)在3.0-8.0的pH下裂解附着在固体基质上的微生物,其中固体基质选自具有形成多个柱的柱结构的固体基质,珠状 固体基质和筛结构的固体基质的水接触角为70-95°,并​​且在其表面上包含至少一个胺基官能团。 在接触步骤之后,洗涤不附着于固体基质和靶微生物以外的材料。 用于分离和扩增核酸的装置包括包括非平面固体基质的腔室,用于加热腔室的加热部分和用于控制腔室加热的温度控制部分。

    탄소나노튜브 가스 센서 및 그 제조 방법
    64.
    发明公开
    탄소나노튜브 가스 센서 및 그 제조 방법 有权
    碳纳米管气体传感器及其制造工艺

    公开(公告)号:KR1020070109765A

    公开(公告)日:2007-11-15

    申请号:KR1020060090465

    申请日:2006-09-19

    Abstract: A carbon nano tube gas sensor and a fabrication process thereof are provided to reduce response time and recovery time by improving a gas reactive property and sensibility. A carbon nano tube gas sensor includes a substrate, an insulating layer, a pair of electrodes(20a,20b), and a carbon nano tube barrier rib(10). The insulating layer is formed on the substrate. The electrodes are formed on the insulating layer. The carbon nano tube barrier rib is protruded higher than the electrodes at a space between the electrodes, so as to be a gas sensing space. The carbon nano barrier rib is formed by patterning and sintering only a portion of the carbon nano tube pastes coated between the electrodes using a photolithography method.

    Abstract translation: 提供了一种碳纳米管气体传感器及其制造方法,以通过改善气体反应性和敏感性来减少响应时间和恢复时间。 碳纳米管气体传感器包括基板,绝缘层,一对电极(20a,20b)和碳纳米管阻挡肋(10)。 绝缘层形成在基板上。 电极形成在绝缘层上。 碳纳米管阻挡肋在电极之间的空间处比电极突出高,从而成为气体感测空间。 碳纳米阻挡肋通过使用光刻方法图案化和烧结涂覆在电极之间的一部分碳纳米管糊而形成。

    전기투석을 이용한 염 농도 조절 장치, 그를 포함하는랩온어칩 및 그를 이용한 염 농도 조절 방법
    65.
    发明授权
    전기투석을 이용한 염 농도 조절 장치, 그를 포함하는랩온어칩 및 그를 이용한 염 농도 조절 방법 有权
    使用电渗析调节盐浓度的装置,包含该盐浓度的实验室,以及使用该浓度调节盐浓度的方法

    公开(公告)号:KR100707191B1

    公开(公告)日:2007-04-13

    申请号:KR1020050044250

    申请日:2005-05-25

    CPC classification number: B01D61/422 B01D61/46 B01L3/5027 G01N2001/4011

    Abstract: 본 발명은 a) 양이온 교환막 및 음이온 교환막에 의해 한정되고, 생분자 추출 챔버, 증폭 챔버, 혼성화 챔버 및 검출 챔버로 구성된 그룹에서 선택되는 반응 챔버; b) 상기 음이온 교환막 및 1차 전극에 의해 한정되고 이온 교환 매질을 포함하는 1차 전극 챔버; 및 c) 상기 양이온 교환막 및 2차 전극에 의해 한정되고 이온 교환 매질을 포함하는 2차 전극 챔버를 포함하는 염 농도 조절 장치를 제공한다. 또한, 본 발명은 상기 장치를 포함하는 랩온어칩 및 상기 장치를 이용한 반응 챔버 내 용액의 염 농도 조절 방법을 제공한다. 본 발명에 따른 장치 및 방법을 사용하면 반응 챔버에 반응의 각 단계마다 펌프 및 밸브를 작동시켜 상이한 농도의 염 용액을 제공할 필요 없이, 전압 인가의 방향, 세기 및 시간 등을 조절함으로써 가역적인 인시츄(
    in situ ) 염 농도 조절이 가능하다.
    염 농도 조절, 반응 챔버, 양이온 교환막, 음이온 교환막, 랩온어칩

    미생물 감지기능이 구비된 로봇청소기
    66.
    发明授权
    미생물 감지기능이 구비된 로봇청소기 有权
    미생물감지기능이구비된로봇청소기

    公开(公告)号:KR100695172B1

    公开(公告)日:2007-03-14

    申请号:KR1020060022325

    申请日:2006-03-09

    Abstract: A robot cleaner having microbe sensing function is provided to fully sterilizing the contaminated area and to normally sterilize the area with less contamination by sensing the contamination level through a microbe. A robot cleaner having microbe sensing function comprises a main body(110) automatically traveling a cleaning area; a dust sucking unit(150) sucking dust in the cleaning area into a predetermined space in the main body; a microbe sensing unit(170) detecting where or not a microbe exists in the cleaning area; and a sterilizing unit(160) operating the sterilizing action according to a microbe contamination measuring signal by the microbe sensing unit. The microbe sensing unit is a gas sensor(171) detecting where or not the microbe exists by sensing a special smell emitted by the microbe. The special smell component is 1-octen-3-lo.

    Abstract translation: 提供具有微生物感测功能的机器人清洁器,以通过微生物感测污染物水平来对污染区域进行完全灭菌并且通常以较少污染对该区域进行杀菌。 具有微生物感测功能的机器人清洁器包括:主体(110),其自动地行进清洁区域; 吸尘单元(150),将清洁区域中的灰尘吸入主体中的预定空间; 微生物感测单元(170),其检测清洁区域中微生物的存在与否; 和根据微生物感测单元根据微生物污染测量信号操作消毒作用的消毒单元(160)。 微生物感测单元是通过感测微生物发出的特殊气味来检测微生物存在或不存在的气体传感器(171)。 特殊气味成分是1-辛烯-3-醇。

    미토콘드리아를 이용한 독성물질유해성 분석방법 및분석장치
    67.
    发明公开
    미토콘드리아를 이용한 독성물질유해성 분석방법 및분석장치 无效
    使用麻醉剂测定毒性的方法及其生物仪器

    公开(公告)号:KR1020070023827A

    公开(公告)日:2007-03-02

    申请号:KR1020050077365

    申请日:2005-08-23

    CPC classification number: G01N33/5079 C12Q1/005 G01N27/00

    Abstract: A method and an apparatus for analyzing toxicity are provided to very easily and electrically measure the toxicity and the degree of harmfulness using the proton amount emitted from mitochondria, and to be easily applied to a lab-on-a-chip. The method for analyzing toxicity using mitochondria comprises a step of contacting a toxic sample to be subject to analysis with mitochondria so ad to electrically measure the proton amount emitted by the mitochondria. The apparatus for analyzing the toxicity comprises a measuring portion which electrically measures the proton amount emitted by the mitochondria, a proton diffusible layer placed at the measuring portion, and a plurality of mitochondria attached to the proton diffusible layer.

    Abstract translation: 提供了一种用于分析毒性的方法和装置,以非常容易且电动地测量使用从线粒体发射的质子量的毒性和危害程度,并且易于应用于片上实验室。 使用线粒体分析毒性的方法包括将待分析的有毒样品与线粒体接触的步骤,以电测量线粒体发射的质子量。 用于分析毒性的装置包括电测量由线粒体发射的质子量的测量部分,放置在测量部分处的质子扩散层和连接到质子扩散层的多个线粒体。

    유체의 pH를 전기화학적으로 조절하기 위한 미세유동장치및 그를 이용하여 pH를 조절하는 방법
    68.
    发明授权
    유체의 pH를 전기화학적으로 조절하기 위한 미세유동장치및 그를 이용하여 pH를 조절하는 방법 有权
    使用pH值调节pH值使用温度控制温度范围pH值调节温度范围

    公开(公告)号:KR100657965B1

    公开(公告)日:2006-12-14

    申请号:KR1020050073273

    申请日:2005-08-10

    Abstract: A microfluidic device is provided to electrochemically and rapidly regulate the pH of a fluid therein by using an ion-exchange material which forms a film simultaneously at the crosslinkage reaction. The microfluidic device comprises: an ion-exchange material(201) which is a cation exchange membrane or an anion exchange membrane; an anode chamber(203), one side of which is composed of one side of the ion-exchange material and has an anode electrode(205) on the edge thereof; and a cathode chamber(209), one side of which is composed of the other side of the ion-exchange material and has a cathode electrode on the edge thereof.

    Abstract translation: 提供微流体装置以通过使用在交联反应时同时形成膜的离子交换材料来电化学和快速调节其中的流体的pH。 微流体装置包括:离子交换材料(201),其为阳离子交换膜或阴离子交换膜; 阳极室(203),其一侧由离子交换材料的一侧构成,并在其边缘具有阳极(205);阳极室 和一个阴极室(209),阴极室(209)的一侧由离子交换材料的另一侧构成,并在其边缘具有阴极。

    SMP를 이용한 독성물질 유해성 측정방법
    69.
    发明授权
    SMP를 이용한 독성물질 유해성 측정방법 失效
    SMP를이용한독성물질유해성측정방법

    公开(公告)号:KR100653938B1

    公开(公告)日:2006-12-06

    申请号:KR1020050097098

    申请日:2005-10-14

    Inventor: 최수형 한정임

    Abstract: A method for measuring harmfulness of toxic substances is provided to be more sensitively measure the harmfulness by effectively measuring the pH change caused by inhibiting inflow of proton from the outside of the sub-mitochondrial particles. The method for measuring harmfulness of toxic substances comprises a step of measuring a pH change induced by contacting an outside electron donor supplying the electrons, such as NADH, FADH2 and NADPH, with an electron transport system of sub-mitochondrial particles while the sub-mitochondrial particles are contacted with a measuring sample, wherein the pH change is measured by electrical signal or color change through a pH indicator.

    Abstract translation: 本发明提供一种测定有害物质的有害性的方法,通过有效地测定由于抑制来自线粒体外粒子的质子的流入而引起的pH变化,能够更灵敏地测定有害性。 测量有毒物质危害性的方法包括测量通过使供给电子的外部电子供体如NADH,FADH2和NADPH与线粒体子线粒子的电子传递系统接触而引起的pH变化的步骤,而线粒体次级线粒体 颗粒与测量样品接触,其中pH变化通过电信号或通过pH指示剂的颜色变化来测量。

    전기투석을 이용한 염 농도 조절 장치, 그를 포함하는랩온어칩 및 그를 이용한 염 농도 조절 방법
    70.
    发明公开
    전기투석을 이용한 염 농도 조절 장치, 그를 포함하는랩온어칩 및 그를 이용한 염 농도 조절 방법 有权
    用于通过电解法调节盐浓度的装置,包括其的包含片的片,以及使用该方法调节盐浓度的方法

    公开(公告)号:KR1020060122196A

    公开(公告)日:2006-11-30

    申请号:KR1020050044250

    申请日:2005-05-25

    Abstract: A device for controlling salt concentration is provided to be able to control the salt concentration in situ without providing salt solution having different concentration by controlling the electrode direction through electrodialysis including a cation exchange membrane and an anion exchange membrane. The salt concentration controlling device comprises: a reaction chamber(201) defined by a cation exchange membrane(205) and an anion exchange membrane(204); a first electrode chamber(202) which is defined by a first electrode(206) and includes ion exchange medium(208); and a second electrode chamber(203) which is defined by a second electrode(207) and includes ion exchange medium(208'). The reaction chamber further comprises an inlet(209) and an outlet(210) to allow a sample solution including biomolecules to be flown in and out.

    Abstract translation: 提供了一种用于控制盐浓度的装置,以便能够通过包括阳离子交换膜和阴离子交换膜的电渗析来控制电极方向,而不提供具有不同浓度的盐溶液来原位控制盐浓度。 盐浓度控制装置包括:由阳离子交换膜(205)和阴离子交换膜(204)限定的反应室(201); 由第一电极(206)限定并包括离子交换介质(208)的第一电极室(202); 和由第二电极(207)限定并包括离子交换介质(208')的第二电极室(203)。 反应室还包括入口(209)和出口(210),以允许包含生物分子的样品溶液进出。

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