Abstract:
A method for isolating a DNA from a microorganism cell is provided to perform effectively microorganism isolation and nucleic acid isolation simultaneously, simplify the isolation process separate nucleic acids without use of additional nucleic acid isolating agent such as chaotropic agent, and perform the isolation by using small devices such as a lap-on-a-chip. A method for isolating a DNA from a microorgansim cell by using a nonplanar solid substrate comprises the steps of: contacting the nonplanar solid substrate with a sample containing microorganisms including bacteria, fungi or viruses at pH 3.0-6.0 to adhere the microorganisms to the solid substrate; removing unadhered materials by washing the solid substrate; and crushing the microorganisms adhered to the solid substrate at pH 3.0-8.0 or pH 11-14, wherein the sample is blood, urine or saliva.
Abstract:
A method for separating a nucleic acid from a microorganism is provided to perform simultaneously and efficiently a microorganism separation and a nucleic acid separation without using a separate nucleic acid separation agent such as a chaotropic agent. And a device for separating and amplifying a nucleic acid is provided to perform a separation and an amplification of the nucleic acid in same or different container. A method for separating a microorganism such as bacteria, fungi and virus comprises the steps of: (a) contacting a non-planar solid substrate with a sample including the microorganism such as blood, urine or saliva under pH of 3.0-6.0 to attach the microorganism to the solid substrate; and (b) lysing the microorganism attached to the solid substrate under the pH of 3.0-8.0, wherein the solid substrate is selected from the group consisting of a solid substrate having a pillar structure where a plurality of pillar are formed, a bead-shaped solid substrate and a sieve-structured solid substrate, has a water contact angle of 70-95° and includes at least one amine-based functional group on the surface thereof. After the contacting step, materials not attached to the solid substrate and other than the target microorganism are washed. A device for separating and amplifying a nucleic acid comprises a chamber including a non-planar solid substrate, a heating portion for heating the chamber, and a temperature controlling portion for controlling the heating of the chamber.
Abstract:
A carbon nano tube gas sensor and a fabrication process thereof are provided to reduce response time and recovery time by improving a gas reactive property and sensibility. A carbon nano tube gas sensor includes a substrate, an insulating layer, a pair of electrodes(20a,20b), and a carbon nano tube barrier rib(10). The insulating layer is formed on the substrate. The electrodes are formed on the insulating layer. The carbon nano tube barrier rib is protruded higher than the electrodes at a space between the electrodes, so as to be a gas sensing space. The carbon nano barrier rib is formed by patterning and sintering only a portion of the carbon nano tube pastes coated between the electrodes using a photolithography method.
Abstract:
본 발명은 a) 양이온 교환막 및 음이온 교환막에 의해 한정되고, 생분자 추출 챔버, 증폭 챔버, 혼성화 챔버 및 검출 챔버로 구성된 그룹에서 선택되는 반응 챔버; b) 상기 음이온 교환막 및 1차 전극에 의해 한정되고 이온 교환 매질을 포함하는 1차 전극 챔버; 및 c) 상기 양이온 교환막 및 2차 전극에 의해 한정되고 이온 교환 매질을 포함하는 2차 전극 챔버를 포함하는 염 농도 조절 장치를 제공한다. 또한, 본 발명은 상기 장치를 포함하는 랩온어칩 및 상기 장치를 이용한 반응 챔버 내 용액의 염 농도 조절 방법을 제공한다. 본 발명에 따른 장치 및 방법을 사용하면 반응 챔버에 반응의 각 단계마다 펌프 및 밸브를 작동시켜 상이한 농도의 염 용액을 제공할 필요 없이, 전압 인가의 방향, 세기 및 시간 등을 조절함으로써 가역적인 인시츄( in situ ) 염 농도 조절이 가능하다. 염 농도 조절, 반응 챔버, 양이온 교환막, 음이온 교환막, 랩온어칩
Abstract:
A robot cleaner having microbe sensing function is provided to fully sterilizing the contaminated area and to normally sterilize the area with less contamination by sensing the contamination level through a microbe. A robot cleaner having microbe sensing function comprises a main body(110) automatically traveling a cleaning area; a dust sucking unit(150) sucking dust in the cleaning area into a predetermined space in the main body; a microbe sensing unit(170) detecting where or not a microbe exists in the cleaning area; and a sterilizing unit(160) operating the sterilizing action according to a microbe contamination measuring signal by the microbe sensing unit. The microbe sensing unit is a gas sensor(171) detecting where or not the microbe exists by sensing a special smell emitted by the microbe. The special smell component is 1-octen-3-lo.
Abstract:
A method and an apparatus for analyzing toxicity are provided to very easily and electrically measure the toxicity and the degree of harmfulness using the proton amount emitted from mitochondria, and to be easily applied to a lab-on-a-chip. The method for analyzing toxicity using mitochondria comprises a step of contacting a toxic sample to be subject to analysis with mitochondria so ad to electrically measure the proton amount emitted by the mitochondria. The apparatus for analyzing the toxicity comprises a measuring portion which electrically measures the proton amount emitted by the mitochondria, a proton diffusible layer placed at the measuring portion, and a plurality of mitochondria attached to the proton diffusible layer.
Abstract:
A microfluidic device is provided to electrochemically and rapidly regulate the pH of a fluid therein by using an ion-exchange material which forms a film simultaneously at the crosslinkage reaction. The microfluidic device comprises: an ion-exchange material(201) which is a cation exchange membrane or an anion exchange membrane; an anode chamber(203), one side of which is composed of one side of the ion-exchange material and has an anode electrode(205) on the edge thereof; and a cathode chamber(209), one side of which is composed of the other side of the ion-exchange material and has a cathode electrode on the edge thereof.
Abstract:
A method for measuring harmfulness of toxic substances is provided to be more sensitively measure the harmfulness by effectively measuring the pH change caused by inhibiting inflow of proton from the outside of the sub-mitochondrial particles. The method for measuring harmfulness of toxic substances comprises a step of measuring a pH change induced by contacting an outside electron donor supplying the electrons, such as NADH, FADH2 and NADPH, with an electron transport system of sub-mitochondrial particles while the sub-mitochondrial particles are contacted with a measuring sample, wherein the pH change is measured by electrical signal or color change through a pH indicator.
Abstract:
A device for controlling salt concentration is provided to be able to control the salt concentration in situ without providing salt solution having different concentration by controlling the electrode direction through electrodialysis including a cation exchange membrane and an anion exchange membrane. The salt concentration controlling device comprises: a reaction chamber(201) defined by a cation exchange membrane(205) and an anion exchange membrane(204); a first electrode chamber(202) which is defined by a first electrode(206) and includes ion exchange medium(208); and a second electrode chamber(203) which is defined by a second electrode(207) and includes ion exchange medium(208'). The reaction chamber further comprises an inlet(209) and an outlet(210) to allow a sample solution including biomolecules to be flown in and out.