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公开(公告)号：KR1020110030030A
公开(公告)日：2011-03-23
申请号：KR1020090087959
申请日：2009-09-17
Applicant: 한국과학기술연구원
CPC classification number: C12M35/08 , C12N5/0653 , C12N15/66
Abstract: PURPOSE: A method for differentiating adipose stem cells to adipocytes using heparin-binding domain(HBD) is provided to induce cell adhesion and to effectively obtain a large amount of adipocytes. CONSTITUTION: A culture container is fixed on a heparin-binding domain(HBD) with an adhesion activity of adipose stem cells. The HBD is fixed in a recombinant protein form with a polypeptide linker by physical absorption. The polypeptide linker is maltose-binding protein(MBP), hydrophobin, or hydrophobic cell penetrating peptides(CPPs). The recombinant protein has an amino acid sequence of sequence number 2.
Abstract translation: 目的：提供使用肝素结合结构域（HBD）将脂肪干细胞分化为脂肪细胞的方法，以诱导细胞粘附并有效获得大量的脂肪细胞。 构成：将培养容器固定在具有脂肪干细胞粘附活性的肝素结合结构域（HBD）上。 通过物理吸收将HBD固定为具有多肽接头的重组蛋白形式。 多肽接头是麦芽糖结合蛋白（MBP），疏水蛋白或疏水细胞穿透肽（CPPs）。 重组蛋白具有序列号2的氨基酸序列。
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Patent Agency Ranking62.发明公开연골 손상의 치료를 위한 세포군집체-하이드로겔-고분자 지지체 복합체, 이의 제조방법 및 이를 유효성분으로 함유하는 연골 손상 치료용 조성물 有权用于培养再生的细胞聚合 - 羟基聚合物复合物，其制备方法和包含其的组合物
公开(公告)号：KR1020100051294A
公开(公告)日：2010-05-17
申请号：KR1020080110395
申请日：2008-11-07
Applicant: 한국과학기술연구원
CPC classification number: C12N5/0655 , A61K35/12 , A61K35/28 , A61K35/32 , A61K35/34 , A61K35/44 , A61K35/50 , A61L27/3817 , A61L27/3852 , A61L27/52 , A61L27/56 , A61L2430/06 , C12N2500/25 , C12N2500/32 , C12N2500/38 , C12N2501/15 , C12N2501/39 , C12N2533/40 , C12N2533/56 , A61K47/30 , A61K47/36 , A61K47/42
Abstract: PURPOSE: A cell colony-hydrogel-polymer supporting complex for a cartilage regeneration, and a cartilage regeneration composition containing thereof are provided to induce the regeneration of a cartilage while maintaining a constant form similar to a natural cartilage. CONSTITUTION: A cell colony-hydrogel-polymer supporting complex for a cartilage regeneration includes a cell colony uniformly dispersed inside a hydrogel matrix. The cell colony-hydrogel complex is dipped in the surface of a polymer supporting body and inserted into a pore to fill the pore. A producing method of the cell colony-hydrogel-polymer supporting complex comprises the following steps: forming the cell colony out of a cell capable of differentiating into a cartilage cell; forming the cell colony-hydrogel complex; and inserting the cell colony-hydrogel complex into a pore after inoculating the colony on the polymer supporting body.
Abstract translation: 目的：提供用于软骨再生的支持复合物的细胞集落水凝胶聚合物和含有其的软骨再生组合物，以在保持类似于天然软骨的恒定形式的同时诱导软骨的再生。 构成：用于软骨再生的支持复合物的细胞集落 - 水凝胶聚合物包括均匀分散在水凝胶基质内的细胞集落。 将细胞集落 - 水凝胶复合物浸入聚合物支撑体的表面并插入孔中以填充孔。 细胞集落 - 水凝胶 - 聚合物支持复合物的制备方法包括以下步骤：从能够分化成软骨细胞的细胞中形成细胞集落; 形成细胞集落 - 水凝胶复合物; 以及在将菌落接种在聚合物支持体上之后将细胞集落 - 水凝胶复合物插入孔中。
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公开(公告)号：KR1020090120241A
公开(公告)日：2009-11-24
申请号：KR1020080046180
申请日：2008-05-19
Applicant: 한국과학기술연구원
CPC classification number: C07K14/245 , C07K14/52 , C07K17/06 , C07K2319/24 , C12N11/06 , C12N11/08 , C12N11/14 , G01N33/54353
Abstract: PURPOSE: A fixation of physiologically active polypeptide with a maltose binding protein(MBP) is provided to maintain biologically activity of the polypeptide and fix the peptide on the hydrophobic surface. CONSTITUTION: A fixation of physiologically polypeptide comprises: a step of fusing the physiologically active polypeptide at carboxy terminal of maltose binding protein(MBP) to obtain a fusion protein; and a step of fixing the fusion protein on a hydrophobic surface by physical adsorption between amino terminal and the hydrophobic surface.
Abstract translation: 目的：提供具有麦芽糖结合蛋白（MBP）的生理活性多肽的固定以维持多肽的生物活性并将肽固定在疏水性表面上。 构成：生理多肽的固定包括：将麦芽糖结合蛋白（MBP）的羧基末端的生理活性多肽融合以获得融合蛋白的步骤; 以及通过氨基末端和疏水性表面之间的物理吸附将融合蛋白固定在疏水性表面上的步骤。
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公开(公告)号：KR1020090004027A
公开(公告)日：2009-01-12
申请号：KR1020070067907
申请日：2007-07-06
Applicant: 한국과학기술연구원
CPC classification number: B29C70/32 , A61F2/06 , A61F2210/0004 , D01D5/0007 , D01D5/04
Abstract: A manufacturing method of a tube type porous scaffold of a double-film structure for artificial blood vessel is provided to have excellent interconnectivity between air bubbles, mechanical stability, transfection and breeding efficiency and to prevent fistula blood at the hypertension state. A manufacturing method of a tube type porous scaffold of a double-film structure for artificial blood vessel comprises steps of: dissolving biodegradable polymer in organic solvent and mixing porogen and manufacturing a polymer/porogen mixed solution; coating a cylindrical shaft with polymer/porogen mixed solution and forming an inner film of a porous coating layer; dissolving the biodegradable polymer in the organic solvent and manufacturing a biodegradable polymer gel; dipping the cylindrical shaft forming the inner film of the porous coating layer, rotating it, falling and radiating the biodegradable polymer gel, winding fiber of a gel state to the inner film of the porous coating layer and forming an outer film of a polymeric fiber layer; separating porosity scaffold of the double-film structure formed in a shaft and removing the organic solvent and the porogen.
Abstract translation: 提供了一种用于人造血管的双层膜结构的管型多孔支架的制造方法，其具有优异的气泡互连性，机械稳定性，转染和繁殖效率，并防止瘘血在高血压状态。 一种用于人造血管的双层膜结构的管型多孔支架的制造方法，包括以下步骤：将可生物降解的聚合物溶解在有机溶剂中并混合致孔剂并制备聚合物/致孔剂混合溶液; 用聚合物/致孔剂混合溶液涂覆圆柱形轴并形成多孔涂层的内膜; 将可生物降解的聚合物溶解在有机溶剂中并制造可生物降解的聚合物凝胶; 浸渍形成多孔涂层的内膜的圆柱形轴，旋转，将可生物降解的聚合物凝胶落下并辐射凝胶状态的卷绕纤维到多孔涂层的内膜，并形成聚合物纤维层的外膜 ; 分离形成在轴中的双膜结构的孔隙支架，并除去有机溶剂和致孔剂。
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公开(公告)号：KR100737167B1
公开(公告)日：2007-07-10
申请号：KR1020060054236
申请日：2006-06-16
Applicant: 한국과학기술연구원
Abstract: Provided is a porous osteochondral composite scaffold with layer structure which has features of bioactivation and mechanical strength suitable to regenerate cartilage and bone simultaneously and method for manufacturing the same. The porous osteochondral composite scaffold comprises: a porous bone regeneration layer which includes biocompatable high molecule and bioactivation ceramic; a porous cartilage regeneration layer which includes biocompatable elastic high molocule. The method for manufacturing a porous osteochondral composite scaffold comprises the steps of: mixing biocompatable high molecule, bioactivation ceramic and lacuna-forming material; press-forming the mixed material from the first step in a mold; bonding the obtained material from the second step by heating to form a porous bone regeneration layer; forming composite material by press-forming gelled mixed material for forming cartilage which dissolves in solution with biocompatable high molecule and lacuna-forming material on one surface of the regenerated layer from the third step; removing solution and lacuna-forming material in the composite material from the fourth step.
Abstract translation: 本发明提供一种具有适于同时再生软骨和骨的具有生物活化和机械强度特征的层状结构的多孔骨软骨复合支架及其制造方法。 多孔骨软骨复合支架包括：多孔骨再生层，其包含生物相容性高分子和生物活化陶瓷; 包含生物相容性弹性高分子量多孔体的多孔软骨再生层。 制备多孔骨软骨复合支架的方法包括以下步骤：混合生物相容性高分子，生物活化陶瓷和腔隙形成材料; 在模具中将来自第一步骤的混合材料压制成形; 通过加热将来自第二步骤的所得材料结合以形成多孔骨再生层; 通过在第三步骤的再生层的一个表面上通过压制成型用于形成软骨的凝胶化混合材料形成复合材料，所述软骨溶解于具有生物相容性高分子和空腔形成材料的溶液中; 从第四步移除复合材料中的溶液和空穴形成材料。
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公开(公告)号：KR1020070006551A
公开(公告)日：2007-01-11
申请号：KR1020060034400
申请日：2006-04-17
Applicant: 한국과학기술연구원
Abstract: A method of preparing porous polymer is provided to improve a cell seeding efficiency by a uniform size of a gap and excellent interconnectedness between gaps, and to prepare simply porous polymer scaffold appropriate for a tissue engineering by an excellent mechanical strength. The method comprises the steps of: manufacturing polymer solution by melting biocompatible polymers in organic solvents; forming polymer gel from non-solvent solutions by spinning the polymer solution obtained in the pre-step to non-solvent stirred by a rotating shaft; forming porous polymer scaffold by making the polymer gel formed in the pre-step wound on the rotating shaft; and removing the organic solvents by drying the porous polymer scaffold obtained in the pre-step.
Abstract translation: 提供一种制备多孔聚合物的方法，以通过间隙的均匀尺寸和间隙之间的优异相互连接来改善细胞接种效率，并且通过优异的机械强度制备适于组织工程的简单多孔聚合物支架。 该方法包括以下步骤：通过在有机溶剂中熔融生物相容性聚合物制备聚合物溶液; 通过将在前步骤中获得的聚合物溶液旋转到通过旋转轴搅拌的非溶剂，从非溶剂溶液形成聚合物凝胶; 通过使前述步骤中形成的聚合物凝胶在旋转轴上形成多孔聚合物支架; 并通过干燥在前步骤中获得的多孔聚合物支架来除去有机溶剂。
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