벼 키다리병 저항성 품종의 대량 검정방법
    72.
    发明授权
    벼 키다리병 저항성 품종의 대량 검정방법 有权
    用于评估抗Bakase疾病的大规模筛选方法

    公开(公告)号:KR101499759B1

    公开(公告)日:2015-03-16

    申请号:KR1020130056065

    申请日:2013-05-16

    Abstract: 본 발명은 2종 이상의 농작물 종자들에 대해 동시에 종자전염병 저항성 품종 여부를 대량으로 검정하는 방법을 제공한다.
    본 발명에 따른 검정 방법은 (a) 처리액이 통과할 수 있는 제1 용기 2개 이상 또는 처리액이 통과할 수 있으면서 2개 이상의 구획을 갖는 제2 용기를 준비하는 단계; (b) 2종 이상의 농작물 종자들을 상기 용기에 치상하되, 서로 다른 종자들은 서로 다른 제1 용기 또는 제2 용기의 서로 다른 구획 내에 치상하는 단계; (c) 2종 이상의 농작물 종자들이 치상된 용기(들)을 종자전염병 병원균 현탁액이 수용되어 있는 욕조(bath)에 동시에 침지하여 접종하는 단계; 및 (d) 제1용기별 또는 제2용기의 각 구획별로 전단계에서 접종된 종자들을 육묘용 상자에 구별하여 파종하는 단계를 포함한다.

    DNA 마커인 RM18166, RM18171 및 Indel 15040을 포함하는 끝동매미충 저항성 유전자 Grh1을 가진 벼 품종 선별용 조성물
    74.
    发明公开
    DNA 마커인 RM18166, RM18171 및 Indel 15040을 포함하는 끝동매미충 저항성 유전자 Grh1을 가진 벼 품종 선별용 조성물 有权
    用于选择含有DNA标记RM18166,RM18171和Indel 15040的含有Grh1基因的绿色稻叶蝉抗性品种的组合物

    公开(公告)号:KR1020150020492A

    公开(公告)日:2015-02-26

    申请号:KR1020130097495

    申请日:2013-08-16

    CPC classification number: C12Q1/6844 C12Q1/6895 C12Q2600/13

    Abstract: The present invention relates to a composition for selecting a rice breed for selecting a rice breed having leafhopper-resistance. The composition includes a primer which detects at least one oligonucleotide or a fraction thereof selected from a group consisting of RM18166, RM18171, and Indel15040 as DNA markers. More specifically, the composition includes one or more primer sets selected from a group consisting of SEQ ID: 1-6. In addition, the present invention relates to a method and a kit for selecting a rice breed having leafhopper-resistance. The method includes the steps of: separating genome DNAs from a rice sample; amplifying a target sequence by performing amplification reaction using one or more primer sets which uses the separated genome DNAs as a template and is selected from a group consisting of SEQ ID: 1-6; and detecting the amplified product. When the method of the present invention is used, rice individuals which have leafhopper-resistance and sensitivity can be efficiently identified by using the makers, which are very closely related to locations of leafhopper-resistant gene.

    Abstract translation: 本发明涉及用于选择具有叶蝉抗性的水稻品种的水稻品种的组合物。 组合物包括检测至少一种选自RM18166,RM18171和Indel15040的寡核苷酸或其部分作为DNA标记的引物。 更具体地,组合物包含选自SEQ ID:1-6所示的一种或多种引物组。 此外,本发明涉及一种用于选择具有叶蝉抗性的水稻品种的方法和试剂盒。 该方法包括以下步骤:从水稻样品中分离基因组DNA; 通过使用使用分离的基因组DNA作为模板的一个或多个引物组进行扩增反应来扩增靶序列,并且选自SEQ ID:1-6; 并检测扩增产物。 当使用本发明的方法时,可以通过使用与叶蝉抗性基因的位置非常密切相关的制造者有效地鉴定具有叶蝉抗性和敏感性的水稻个体。

    벼 현미 종피색으로부터 안토시아닌을 함유하는 흑미 선별용 프라이머 및 이를 이용한 흑미 품종 선별 방법
    77.
    发明公开
    벼 현미 종피색으로부터 안토시아닌을 함유하는 흑미 선별용 프라이머 및 이를 이용한 흑미 품종 선별 방법 有权
    用于选择在大米颗粒中分离蒽醌的颜色的选择集及其选择方法

    公开(公告)号:KR1020140046570A

    公开(公告)日:2014-04-21

    申请号:KR1020120110742

    申请日:2012-10-05

    CPC classification number: C12Q1/686 C12Q2600/13

    Abstract: The present invention provides: a primer set for differentiating the seed coat color of black rice containing anthocyanin and other brown rice seed coat colors; a kit for differentiating the seed coat color of black rice containing anthocyanin and other brown rice seed coat colors; and a method of differentiating the seed coat color of black rice containing anthocyanin and other brown rice seed coat colors by performing an amplification reaction using the primer set. By using the primer set of the present invention, a user can differentiate black rice in a seedling condition compared to an existing sorting method of confirming the color of seed coats after the vegetative growth, discharging water, and grain filling; and the homomorphy and heteromorphy of characteristics can be possible, thereby improving the breeding efficiency utilizing the juvenile selection of black rice and minimum resources. [Reference numerals] (AA) Boseokheugchal(black rice); (BB) No.244 of Miryang(white rice); (CC) Approximately 450 bp; (DD) Approximately 215 bp

    Abstract translation: 本发明提供了一种用于分化含有花青素和其他糙米种衣色的黑米的种皮颜色的引物组; 一种用于区分含有花青素和其他糙米种皮颜色的黑米的种皮颜色的试剂盒; 以及通过使用引物组进行扩增反应来区分含有花青素和其他糙米种皮颜色的黑米的种皮颜色的方法。 通过使用本发明的引物组,与用于确认营养生长后的种皮颜色的现有分类方法相比,使用者能够在苗期条件下区分黑米,排出水和灌浆; 特色的同性异体和异质性是可能的,从而通过青少年选择黑米和最低限度的资源提高育种效率。 (AA)Boseokheugchal(黑米); (BB)米阳244号(白米); (CC)约450bp; (DD)约215bp

    벼 줄무늬잎마름병 저항성 유전자 Stv-bi를 가진 벼 품종 선별용 프라이머 및 이를 이용한 벼 품종 선별 방법
    79.
    发明公开
    벼 줄무늬잎마름병 저항성 유전자 Stv-bi를 가진 벼 품종 선별용 프라이머 및 이를 이용한 벼 품종 선별 방법 无效
    用于选择耐变性的含有STV-BI基因的稻瘟病病毒的选择及其选择方法

    公开(公告)号:KR1020130091434A

    公开(公告)日:2013-08-19

    申请号:KR1020120012710

    申请日:2012-02-08

    CPC classification number: C12Q1/6895 C12Q1/6844 C12Q2600/13 C12Q2600/158

    Abstract: PURPOSE: A method of selecting a breed that is resistive to a rice stripe disease is provided to efficiently separate a breed that is resistive and sensitive with respect to the rice stripe disease by using a marker, which is closely related with a location of a rice stripe disease-resistant gene. CONSTITUTION: A pair of primers for selecting a breed of rice resistive against a rice stripe disease is selected from the group consisting of a primer pair represented by SEQ ID NO: 1 and 2; a primer pair represented by SEQ IE NO: 3 and 4; a primer pair represented by SEQ IE NO: 5 and 6; a primer pair represented by SEQ IE NO: 7 and 8; a primer pair represented by SEQ IE NO: 9 and 10; a primer pair represented by SEQ IE NO: 11 and 12; a primer pair represented by SEQ IE NO: 13 and 14; a primer pair represented by SEQ IE NO: 15 and 16; a primer pair represented by SEQ IE NO: 17 and 18; a primer pair represented by SEQ IE NO: 19 and 20; and a primer pair represented by SEQ IE NO: 21 and 22. A method of selecting a rice stripe disease-resistant breed comprises the steps of: (i) isolating DNA from a rice sample; (ii) amplifying a target sequence by performing an amplification reaction by having the isolated DNA as a template and using the at least one primer pair selected from above; and (iii) detecting the amplification product.

    Abstract translation: 目的:提供一种选择抗稻瘟病抗性品种的方法,通过使用与稻米位置密切相关的标记,有效地分离出与水稻条纹病有关的抗性和敏感性品种 条纹抗病基因。 构成:从由SEQ ID NO:1和2表示的引物对组成的组中选择一对用于选择抗稻瘟病抗性的水稻品种的引物; 由SEQ ID NO:3和4表示的引物对; 由SEQ ID NO:5和6表示的引物对; 由SEQ ID NO:7和8表示的引物对; 由SEQ ID NO:9和10表示的引物对; 由SEQ ID NO:11和12表示的引物对; 由SEQ ID NO:13和14表示的引物对; 由SEQ ID NO:15和16表示的引物对; 由SEQ ID NO:17和18表示的引物对; 由SEQ ID NO:19和20表示的引物对; 和由SEQ ID NO:21和22表示的引物对。选择水稻条纹抗病品种的方法包括以下步骤:(i)从稻样品中分离DNA; (ii)通过使分离的DNA作为模板并使用选自上述的至少一种引物对进行扩增反应来扩增靶序列; 和(iii)检测扩增产物。

    가바와 아미노산이 다량 포함된 거대배아미 밀양263호의 쌀겨 추출물 및 이 추출물을 포함하는 조성물
    80.
    发明授权
    가바와 아미노산이 다량 포함된 거대배아미 밀양263호의 쌀겨 추출물 및 이 추출물을 포함하는 조성물 有权
    大米提取物,MILYANG263,含有GABA和氨基酸及其提取物的组合物

    公开(公告)号:KR101278600B1

    公开(公告)日:2013-06-25

    申请号:KR1020110146547

    申请日:2011-12-29

    Abstract: PURPOSE: A method of effectively producing GABA(Gamma-aminobutyric acid) and amino acid and a composition thereof are provided to maximize the production of GABA and amino acid from unpolished rice of giant embryonic rice and rice bran. CONSTITUTION: Rice bran of giant embryonic rice Milyang No. 263 is mixed with a solvent and extracted by ultrasonic waves. The solvent used in the extraction is water, C1-C4 alcohol, ethyl acetate, acetone, or a mixed solvent thereof. [Reference numerals] (AA) Properties; (BB) Varieties; (CC) Ordinary rice; (DD) Superb rice; (EE) Black glutinous rice; (FF) Early maturing black glutinous rice; (GG) Giant embryonic rice; (HH) Embryo; (II) Giant embryonic glutinous rice; (JJ) Giant black glutinous rice; (KK) Mylyang No. 263

    Abstract translation: 目的:提供有效生产GABA(γ-氨基丁酸)和氨基酸及其组合物的方法,以最大限度地利用巨型胚胎米和米糠未糙米的GABA和氨基酸的产生。 构成:将巨型胚胎米苗263号的米糠与溶剂混合并通过超声波提取。 萃取中使用的溶剂是水,C1-C4醇,乙酸乙酯,丙酮或其混合溶剂。 (附图标记)(AA)性质; (BB)品种; (CC)普通米; (DD)精湛的米饭; (EE)黑糯米; (FF)早熟黑糯米; (GG)巨型大米; (HH)胚胎; (二)大型糯米糯米; (JJ)巨型黑糯米; (KK)Mylyang No.263

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