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公开(公告)号：KR101286721B1
公开(公告)日：2013-07-16
申请号：KR1020090049866
申请日：2009-06-05
Applicant: 한국과학기술연구원
CPC classification number: C07K14/76 , A61K47/643 , C07K14/765 , C07K2319/20
Abstract: 본 발명은 폴리-시스테인 펩티드 융합 재조합 알부민 및 이의 제조방법에 관한 것으로서, 보다 상세하게는 알부민의 N-말단과 C-말단에 약물 결합에 이용될 수 있는 시스테인의 갯수가 증폭되어 있는 재조합 알부민 및 이의 제조방법에 관한 것이다. 본 발명의 재조합 알부민은 약물 전달체용으로 사용시에 알부민-약물 컨쥬게이션 효율을 향상시키므로 다량의 약물을 표적 조직에 효과적으로 전달할 수 있고, 융합된 시스테인의 갯수를 조절함으로써 알부민 단일 개체당 결합되는 약물의 갯수를 조절할 수 있으므로 기존 알부민 전달체의 부작용을 개선한 우수한 약물 전달체로 사용될 수 있으며, 또한, 이러한 재조합 알부민을 분자 영상용 형광체 및 조영제 등과 결합시켜 비침습적으로 실시간 질병 진단 및 치료와 신약의 스크리닝에 활용할 수 있다.
폴리-시스테인 펩티드, 재조합 알부민, 약물 결합, 약물 전달, 분자 영상-
Patent Agency Ranking
公开(公告)号：KR1020120079299A
公开(公告)日：2012-07-12
申请号：KR1020110000526
申请日：2011-01-04
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/37 , G01N33/533 , G01N33/54346 , G01N2500/20
Abstract: PURPOSE: A biosensor for detecting Atg4B protease containing a polypeptide specifically recognized by Atg4B protease is provided to effectively detect the protease. CONSTITUTION: A biosensor for detecting Atg4B(Arabidopsis autophagy-related proteins 4b) protease contains polypeptides with 3-20 amino acid residues having an amino acid sequence of chemical formula 1(R1-R2-Gly). In chemical formula 1, R1 is Thr or Val; and R2 is Phe or Tyr. The biosensor contains polypeptides and polymer-linked conjugate for forming nanoparticles. A composition for Atg4B protease reaction contains the biosensor.
Abstract translation: 目的：提供用于检测Atg4B蛋白酶的生物传感器，该蛋白酶含有由Atg4B蛋白酶特异识别的多肽，以有效检测蛋白酶。 构型：用于检测Atg4B（拟南芥自噬相关蛋白4b）蛋白质的生物传感器含有具有化学式1（R1-R2-Gly）氨基酸序列的3-20个氨基酸残基的多肽。 在化学式1中，R 1是Thr或Val; R2是Phe或Tyr。 生物传感器包含用于形成纳米颗粒的多肽和聚合物连接的缀合物。 用于Atg4B蛋白酶反应的组合物含有生物传感器。
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73.发明公开
公开(公告)号：KR1020110140014A
公开(公告)日：2011-12-30
申请号：KR1020100060180
申请日：2010-06-24
Applicant: 한국과학기술연구원
Abstract: PURPOSE: A fusion protein of ferritin and GALA peptides and a cage protein containing the same are provided to effectively release drug under a weak acid condition and to be applied as a biosensor. CONSTITUTION: A fusion protein contains a GALA(Glu-Ala-Leu-Ala) motif containing an amino acid sequence of sequence number 13 linked to C-terminal of a polypeptide of a fragment containing ferritin protein, A-helix, B-helix, C-helix, and D-helix or mutant thereof. The ferritin is denoted by amino acid sequence number 1. The fragment is denoted by amino acid sequence 3. The GALA motif contains 4-100 amino acids of amino acid sequence number 13 and is denoted by amino acid sequence number 5 or 7. A cage protein contains the fusion protein.
Abstract translation: 目的：提供铁蛋白和GALA肽的融合蛋白和含有其的笼状蛋白，以在弱酸条件下有效释放药物，并作为生物传感器使用。 构成：融合蛋白含有含有序列号13的氨基酸序列的GALA（Glu-Ala-Leu-Ala）基序，其与含有铁蛋白，A-螺旋，B-螺旋的片段的多肽的C-末端连接， C-螺旋和D-螺旋或其突变体。 铁蛋白由氨基酸序列号1表示。该片段由氨基酸序列3表示.GALA基序含有氨基酸序列号13的4-100个氨基酸，并由氨基酸序列号5或7表示。笼 蛋白质含有融合蛋白。
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公开(公告)号：KR1020110139456A
公开(公告)日：2011-12-29
申请号：KR1020100059567
申请日：2010-06-23
Applicant: 한국과학기술연구원
CPC classification number: A61K47/42 , C07K14/435 , C07K14/47 , C07K2319/21 , C12Q1/37 , G01N33/542 , G01N2333/96466 , G01N2333/96494 , G01N2510/00
Abstract: PURPOSE: A fusion protein containing low molecular heat shock protein and a cage protein formed by the same are provided to emit high fluorescence signal. CONSTITUTION: A fusion protein contains a recognition site of a protease and histidine polymers linked to low molecular heat shock protein(HSP). The low molecular HSP is a polypeptide of amino acid sequence 6 or fragment or mutant thereof. The protease is caspase, matrix metalloproteinase(MMP), cathepsin S, or viral protease. The recognition site is a polypeptide having 4-20 amino acid residue containing a cysteine residue. A cage protein contains the fusion proteins.
Abstract translation: 目的：提供含有低分子量热休克蛋白和由其形成的笼状蛋白的融合蛋白以发射高荧光信号。 构成：融合蛋白含有与低分子量热休克蛋白（HSP）相连的蛋白酶和组氨酸聚合物的识别位点。 低分子HSP是氨基酸序列6或其片段或突变体的多肽。 蛋白酶是半胱天冬酶，基质金属蛋白酶（MMP），组织蛋白酶S或病毒蛋白酶。 识别位点是具有含有半胱氨酸残基的4-20个氨基酸残基的多肽。 笼状蛋白含有融合蛋白。
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公开(公告)号：KR1020110061432A
公开(公告)日：2011-06-09
申请号：KR1020090118079
申请日：2009-12-01
Applicant: 한국과학기술연구원
CPC classification number: A61K49/0017 , A61K49/0021 , A61K49/0054 , A61K49/1824 , G01N33/49 , G01N33/58
Abstract: PURPOSE: Aqueous-dispersed chemical-luminescent nanoparticles, a producing method thereof, and a use thereof are provided to accurately and extensively diagnose active oxygen related disease by directly imaging hydrogen peroxide. CONSTITUTION: Aqueous-dispersed chemical-luminescent nanoparticles contain the following: 0.01~3wt% of core containing a hydrophobic chemical-luminescent substance; 0.5~30wt% of surfactant surrounding the core; and 67~99.49wt% water. The luminescent wavelength of the hydrophobic chemical-luminescent substance is 650~900nm. The hydrophobic chemical-luminescent substance is formed with a peroxyoxalate derivative and a fluorescent material. The surfactant is poloxamer.
Abstract translation: 目的：通过直接成像过氧化氢，提供水分散化学发光纳米颗粒及其制备方法及其用途，以准确和广泛地诊断活性氧相关疾病。 构成：含水分散化学发光纳米粒子含有以下物质：含有疏水性化学发光物质的核心的0.01〜3重量％ 核心表面活性剂占0.5〜30wt％; 和67〜99.49重量％的水。 疏水化学发光物质的发光波长为650〜900nm。 疏水性化学发光物质由过氧草酸酯衍生物和荧光物质形成。 表面活性剂是泊洛沙姆。
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公开(公告)号：KR1020100084039A
公开(公告)日：2010-07-23
申请号：KR1020090003431
申请日：2009-01-15
Applicant: 한국과학기술연구원
CPC classification number: C12N15/70 , C07K2319/35
Abstract: PURPOSE: A plasmid for expressing protein containing thioredoxin as a fusion partner is provided to easily remove thioredoxin and to massively produce a target protein. CONSTITUTION: A pET-32-S(-) plasmid contains a multi-cloning site for inserting thioredoxin gene, thrombin recognition site, S-tag coding region, and a gene encoding a target protein. A method for producing the target protein comprises: a step of inserting the gene encoding target protein into a multi-cloning site of a plasmid; a step of transforming the plasmid to E.coli and culturing; and a step of treating thrombine.
Abstract translation: 目的：提供用于表达含有硫氧还蛋白作为融合配偶体的蛋白质的质粒，以容易地除去硫氧还蛋白并大量产生靶蛋白。 构成：pET-32-S（ - ）质粒含有用于插入硫氧还蛋白基因，凝血酶识别位点，S标签编码区和编码靶蛋白的基因的多克隆位点。 制备靶蛋白的方法包括：将编码靶蛋白的基因插入质粒的多克隆位点的步骤; 将质粒转化大肠杆菌并培养的步骤; 和治疗血栓的步骤。
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