公开(公告)号：KR1020130010040A

公开(公告)日：2013-01-24

申请号：KR1020110067331

申请日：2011-07-07

Applicant: 한국식품연구원

Inventor： 이나리 ,  최순영 ,  설자영 ,  구민선 ,  전향숙 ,  김현정 ,  장현주

IPC: C12N15/11 ,  C12N15/63 ,  G01N33/53 ,  G01N33/15

Abstract: PURPOSE: A recombinant expression vector for searching a therapeutic agent for treating obesity or diabetes and a searching method using the same are provided to develop the therapeutic agent. CONSTITUTION: A leptin receptor promoter has a base of sequence number 2. The promoter contains a base selected from sequence numbers 3-15. The promoter contains a base of sequence number 9 or 1. A recombinant vector contains the promoter and a reporter gene. The reporter gene is luciferase, HRP(horseraddish peroxidase) or alkaline phosphatase. A cell line transformed with the recombinant vector is ORp-HEK293(KCTC 11920BP). A method for searching a therapeutic agent for treating obesity or diabetes comprises: a step of treating a candidate material for treating obesity or diabetes to a cell line and a step of confirming expression level of the leptin receptor.

Abstract translation: 目的：提供用于搜索治疗剂用于治疗肥胖或糖尿病的重组表达载体和使用其的检索方法以开发治疗剂。 构成：瘦素受体启动子具有序列号2的碱基。启动子含有选自序列号3-15的碱基。 启动子含有序列号9或1的碱基。重组载体含有启动子和报告基因。 报道基因是荧光素酶，HRP（辣根过氧化物酶）或碱性磷酸酶。 用重组载体转化的细胞系是ORp-HEK293（KCTC 11920BP）。 用于搜索治疗剂用于治疗肥胖或糖尿病的方法包括：将治疗肥胖症或糖尿病的候选物质治疗至细胞系的步骤和确认瘦素受体表达水平的步骤。

公开(公告)号：KR101174821B1

公开(公告)日：2012-08-20

申请号：KR1020100030534

申请日：2010-04-02

Applicant: 한국식품연구원

Inventor： 최성욱 ,  전향숙 ,  장현주 ,  이영기

IPC: G01F1/74 ,  G01F1/00 ,  G01N21/01 ,  G01N21/00

Abstract: 본발명이해결하려는과제는미세유로채널에유입된유체의굴절률변화를이용하여정확한유량을측정할수 있는유체센서를제공하는것이다. 본발명굴절률변화를이용한유체센서는미세유로채널에광을조사하기위한광원과; 상기광원으로부터방출된광이미세유로채널내부에존재하는유체또는기포에의해굴절되어변화된광량을검출하기위한광검출기로구성된것을특징으로한다. 본발명에의하면, 미세유로채널내부에유입된유체에기포가존재하는지여부를용이하게알 수있는장점이있다. 또한, 미세유로채널내부에유입된유체에일정부피의기포를주입하여유체의정확한유속을측정할수 있으며, 상기측정된유속을이용하여미세유로채널내부에유입된유체에기포가존재하는경우에도유체의정확한유량을측정할수 있으므로미세유로채널에유입된유체의유속과유량을정확하게측정하여제어할수 있는장점이있다.

公开(公告)号：KR1020120076908A

公开(公告)日：2012-07-10

申请号：KR1020100138677

申请日：2010-12-30

Applicant: 한국식품연구원

Inventor： 전향숙 ,  최성욱 ,  이나리 ,  장현주 ,  김현정 ,  이영기

IPC: G01N21/27

Abstract: PURPOSE: A surface plasmon resonance sensor system is provided to prevent the distortion of spectrum and to rapidly calculate a sensing result without a separate signal processing process on a real time basis. CONSTITUTION: A surface plasmon resonance sensor system comprises a sensor unit(130), a light source u nit, and a detector(140). The surface plasmon resonance is excited in the sensor unit. The light source unit provides parallel rays having a uniform luminous intensity distribution along at least one direction to the sensor unit. The detector detects lights changed by the surface plasmon resonance in the sensor unit. The light source unit comprises a plurality of light sources(110) and window members(120). The plurality of light sources individually emits lights. The window member is arranged on an optical path of the lights emitted from the light source. A window hole(122) is formed in the window member.

Abstract translation: 目的：提供表面等离子体共振传感器系统，以防止频谱失真，并在没有单独的信号处理过程的情况下快速计算感测结果。 构成：表面等离子体共振传感器系统包括传感器单元（130），光源和检测器（140）。 在传感器单元中激发表面等离子体共振。 光源单元提供沿传感器单元沿至少一个方向具有均匀的发光强度分布的平行光线。 检测器检测由传感器单元中的表面等离子体共振改变的光。 光源单元包括多个光源（110）和窗口部件（120）。 多个光源分别发光。 窗构件布置在从光源发射的光的光路上。 在窗构件中形成有窗孔（122）。

公开(公告)号：KR1020120029748A

公开(公告)日：2012-03-27

申请号：KR1020100091771

申请日：2010-09-17

Applicant: 한국식품연구원

Inventor： 전향숙 ,  최성욱 ,  이나리 ,  장현주 ,  이영기

IPC: C12M1/38 ,  C12Q1/68

CPC classification number: B01L7/52 ,  B01L7/5255 ,  B01L2300/0803 ,  B01L2300/0829 ,  B01L2300/168 ,  B01L2300/1844 ,  B01L2300/1861 ,  B01L2300/1872 ,  C12Q1/6844 ,  C12M1/38

Abstract: PURPOSE: A contactless heating type gene amplification system is provided to amplify various samples and perform temperature cycling at high speed by controlling temperature at every step and enhance cooling efficiency by forming a non-heating part between dissociation part and annealing part. CONSTITUTION: A contactless heating type gene amplification system comprises a plurality of optical source modules which are formed along the columnar direction, a halogen lamp on which ruby or gold is evaporated, sample chambers which are formed to be symmetrical based on center of a rotating unit, the rotating unit which one side is composed of transmittable glass of infrared ray, a non-heating part(14) which does not include separate optical source module and is formed between dissociation part(10a) which performs dissociation step and an annealing part(10b) which performs annealing step, and an extension part(10c) which performs extension phase.

Abstract translation: 目的：提供无接触加热型基因扩增系统，通过控制各步骤的温度，通过在解离部分和退火部分之间形成非加热部分，高效地放大各种样品并进行高速循环。 构成：非接触式加热型基因扩增系统包括沿着柱状方向形成的多个光源模块，其中红宝石或金蒸发的卤素灯，基于旋转单元的中心形成为对称的样品室 ，一侧由红外线透射玻璃构成的旋转单元，不具有分离光源模块的非加热部（14），形成在进行解离步骤的解离部（10a）和退火部（ 10b），以及延伸部（10c），其进行延伸相。

公开(公告)号：KR1020100131195A

公开(公告)日：2010-12-15

申请号：KR1020090049968

申请日：2009-06-05

Applicant: 한국식품연구원

Inventor： 최성욱 ,  전향숙 ,  장현주 ,  신장규

IPC: B82B3/00 ,  C01B31/02 ,  G01N33/53

CPC classification number: C01B32/16 ,  B82B3/0009 ,  C01B2202/02 ,  G01N33/53

Abstract: PURPOSE: A method for manufacturing a single-walled carbon nanotube(SWNT) is provided to improve reproducibility and accuracy of signal using single-walled carbon nanotube which is arranged at one side. CONSTITUTION: A method for manufacturing a single-walled carbon nanotube(SWNT) comprises a first step for dropping an SWNT solution dispersed in chloroform, on the surface of Langmuir-Blodgett trough filled with distilled water and evaporating the chloroform to leave only SWNT; and a second step for arranging the SWNT by moving one side barrier.

Abstract translation: 目的：提供单壁碳纳米管（SWNT）的制造方法，以提高使用单壁碳纳米管排列在一侧的信号的再现性和准确性。 构成：制造单壁碳纳米管（SWNT）的方法包括：将分散在氯仿中的SWNT溶液滴在填充有蒸馏水的Langmuir-Blodgett槽的表面上并蒸发氯仿以仅留下SWNT的第一步骤; 以及通过移动一侧屏障来布置SWNT的第二步骤。

公开(公告)号：KR1020080100645A

公开(公告)日：2008-11-19

申请号：KR1020070046606

申请日：2007-05-14

Applicant: 한국식품연구원

Inventor： 최성욱 ,  전향숙 ,  장현주 ,  김현정 ,  김현정 ,  이나리 ,  김재호 ,  이재혁

IPC: G01N33/53 ,  G01N33/50 ,  G01N33/48

Abstract: A surface plasmon resonance sensor chip using molecular imprinting technique is provided to detect easily fungal toxin zearalenone or a derivative of the fungal toxin zearalenone using an electrochemical method. A surface plasmon resonance sensor chip using molecular imprinting technique for detecting easily fungal toxin zearalenone or a derivative of the fungal toxin zearalenone contains a substrate(10) in which a metal layer is formed, a metal layer(20) formed on a polymer layer including a binding site(21) combined with a zearalenone or a derivative of the zearalenone. The polymer layer is manufactured by steps of: mixing a monomer polymerized if electricity is applied and a template for forming a binding site in which the zearalenone or the derivative of the zearalenone is combined; forming a polymer layer by applying the electricity after injecting the monomer in which the template is mixed on the substrate; and forming the binding site combined with a zearalenone or a derivative of the zearalenone by removing the template from the polymer layer.

Abstract translation: 提供使用分子印迹技术的表面等离子体共振传感器芯片，以使用电化学方法检测玉米赤霉烯真菌毒素或玉米赤霉菌真菌毒素的衍生物。 使用分子印迹技术的表面等离子体共振传感器芯片容易地检测玉米赤霉烯真菌毒素或玉米赤霉菌素的衍生物的真菌毒素含有其中形成金属层的基底（10），在聚合物层上形成的金属层（20），包括 与玉米赤霉烯酮或玉米赤霉烯酮衍生物结合的结合位点（21）。 聚合物层通过以下步骤制造：混合施加电力时聚合的单体和用于形成玉米赤霉烯酮或玉米赤霉烯酮衍生物的结合位点的模板; 通过在将基板上混合有模板的单体注入后施加电力来形成聚合物层; 并通过从聚合物层去除模板，形成与玉米赤霉烯酮或玉米赤霉烯酮衍生物结合的结合位点。