公开(公告)号：DE10016377B4

公开(公告)日：2009-01-08

申请号：DE10016377

申请日：2000-04-04

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN ,  HOFFMANN JUERGEN ,  STORZ RAFAEL ,  ULRICH HEINRICH ,  BEWERSDORF JOERG ,  BIRK HOLGER

IPC: G02B5/30 ,  G02B27/10 ,  G02B6/287 ,  G02B21/00 ,  G02B21/06 ,  G02B27/28 ,  G02F1/29 ,  G02F1/33

Abstract: The present invention concerns an apparatus for combining light from at least two laser light sources, preferably in the context of confocal scanning microscopy, and in order to make laser light sources of low output power usable as light sources, in particular for confocal scanning microscopy, is characterized in that the light from the laser light sources has at least approximately the same wavelength; and that at least one beam combining unit that combines the light beams in at least largely lossless fashion is provided.

公开(公告)号：DE59909861D1

公开(公告)日：2004-08-12

申请号：DE59909861

申请日：1999-05-07

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN ,  ZAPF THOMAS ,  HUG BILL

IPC: G02B21/00 ,  H01S3/10

公开(公告)号：DE19906763B4

公开(公告)日：2004-07-22

申请号：DE19906763

申请日：1999-02-17

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN

IPC: G02B21/00

公开(公告)号：DE19853407C2

公开(公告)日：2003-09-11

申请号：DE19853407

申请日：1998-11-19

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN ,  SCHREIBER FRANK

IPC: G02B21/00

公开(公告)号：AT244414T

公开(公告)日：2003-07-15

申请号：AT99962046

申请日：1999-11-05

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN ,  SCHREIBER FRANK

IPC: G02B21/00

公开(公告)号：DE10150542A1

公开(公告)日：2003-04-30

申请号：DE10150542

申请日：2001-10-12

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN ,  HOFFMANN JUERGEN

IPC: G01N21/64 ,  G02B21/16

Abstract: The invention discloses a fluorescence microscope comprising a light source that emits excitation light for illumination of a specimen, means for defining a two-dimensional search region for the excitation and detection wavelengths, means for selecting a subregion from the search region, at least one detector that detects detected light proceeding from the specimen, and a display for displaying an image of at least a portion of the specimen. Furthermore the invention discloses a method for fluorescence microscopy.

公开(公告)号：GB2368743B

公开(公告)日：2003-03-26

申请号：GB0120663

申请日：2001-08-24

Applicant: LEICA MICROSYSTEMS

Inventor： STORZ RAFAEL ,  ENGELHARDT JOHANN ,  BIRK HOLGER

IPC: G02B26/06 ,  G02B21/00 ,  G02B21/06 ,  G02F1/01 ,  H01S3/00

Abstract: A method for illuminating an object with light (2) from a laser light source (3), preferably in a confocal scanning microscope (1). With the method according to the invention, it is possible to reduce the coherence length of the laser light, so that disruptive interference phenomena can be substantially eliminated. Should interference phenomena nevertheless be formed, these are to be influenced in such a way that they have no effect on the detection. The method according to the invention is characterized in that the phase angle of the light field is varied by a modulator in such a way that interference phenomena do not occur in the optical beam path, or occur only to an undetectable extent, within a predeterminable time interval.

公开(公告)号：DE10139920A1

公开(公告)日：2003-03-06

申请号：DE10139920

申请日：2001-08-14

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN

IPC: G02B21/00 ,  G02B21/06

Abstract: A scanning microscope possesses at least one illumination source for emitting an illuminating beam that is conveyed via a beam deflection device and an optical system to a specimen and scans the latter, the beam deflection device defining at least one illuminating beam rotation point. A device for axial displacement in particular of the beam deflection device, or of a lens preceding the objective, is provided for imaging of an image of the illuminating beam rotation point into the pupil of the objective.

公开(公告)号：GB2367701B

公开(公告)日：2002-12-24

申请号：GB0116559

申请日：2001-07-06

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN

IPC: G02B21/06 ,  G02B21/00 ,  G02F1/11 ,  G02F1/33 ,  G02B6/27

Abstract: The present invention concerns an apparatus for coupling light (1) of at least one wavelength of a laser light source (2) into an optical assemblage (3), preferably into a confocal scanning microscope, having an optically active component (4) that serves in particular to select the wavelength and to set the power of the coupled-in light (5). To ensure that changes in the power and/or wavelength of the laser light source do not affect the power of the light (5) coupled into the optical assemblage, the apparatus according to the present invention is characterized in that in order to influence the coupled-in light (5), the component (4) serves as the adjusting element of a control system (11).

公开(公告)号：DE59708457D1

公开(公告)日：2002-11-21

申请号：DE59708457

申请日：1997-12-12

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN ,  IHRIG CHRISTIANE

IPC: G02B21/00 ,  G02B21/36