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71.
公开(公告)号:DE102013213781A1
公开(公告)日:2014-09-25
申请号:DE102013213781
申请日:2013-07-15
Applicant: LEICA MICROSYSTEMS
Inventor: KNEBEL WERNER , SIECKMANN FRANK , FOUQUET WERNHER
Abstract: Die Erfindung betrifft ein Verfahren, bei dem eine Probe mit Manipulationslicht manipuliert wird und bei dem die Probe mittels SPIM-Technik unter Beleuchtung mit Beleuchtungslicht, insbesondere Anregungslicht zur Fluoreszenzanregung, in Form eines Beleuchtungslichtblatts abgebildet wird. Das Verfahren zeichnet sich dadurch aus, dass sowohl das Manipulationslicht, als auch das Beleuchtungslicht durch dasselbe Objektiv, das in einer Objektivarbeitsposition angeordnet ist, oder durch unterschiedliche Objektive, die nacheinander in eine Objektivarbeitsposition gebracht werden, fokussiert wird und dass das Manipulationslicht und/oder das Beleuchtungslicht nach dem Durchlaufen des Objektivs mittels einer Umlenkeinrichtung derart umgelenkt wird, dass es sich unter einem von Null Grad verschiedenen Winkel zur optischen Achse des Objektivs ausbreitet.
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公开(公告)号:DE502004012202D1
公开(公告)日:2011-03-31
申请号:DE502004012202
申请日:2004-07-26
Applicant: LEICA MICROSYSTEMS
Inventor: KNEBEL WERNER
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公开(公告)号:DE502004009281D1
公开(公告)日:2009-05-14
申请号:DE502004009281
申请日:2004-09-23
Applicant: LEICA MICROSYSTEMS
Inventor: ULRICH HEINRICH , KNEBEL WERNER , MOELLMANN KYRA , EUTENEUER PETER
Abstract: A microscope with evanescent sample illumination comprises a device for optically manipulating a sample.
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公开(公告)号:DE502004008954D1
公开(公告)日:2009-03-19
申请号:DE502004008954
申请日:2004-09-23
Applicant: LEICA MICROSYSTEMS
Inventor: ULRICH HEINRICH , KNEBEL WERNER , MOELLMANN KYRA , EUTENEUER PETER
Abstract: A microscope comprises an objective and a light source that produces an illumination light beam-in particular for evanescent illumination of a sample, which exhibits a focus in the plane of the objective pupil. To adjust the penetration depth, an adjustment mechanism is provided with which the spatial position of the focus within the plane of the objective pupil may be changed.
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公开(公告)号:DE502004008431D1
公开(公告)日:2008-12-18
申请号:DE502004008431
申请日:2004-09-22
Applicant: LEICA MICROSYSTEMS
Inventor: HECKER ANDREAS , KNEBEL WERNER , MOELLMANN KYRA , ULRICH HEINRICH
Abstract: A microscope with a light source that produces an illumination light beam for evanescently illuminating a sample includes an adjustment mechanism with which the polarization of the illumination light beam may be changed.
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公开(公告)号:AT413620T
公开(公告)日:2008-11-15
申请号:AT04766837
申请日:2004-09-22
Applicant: LEICA MICROSYSTEMS
Inventor: HECKER ANDREAS , KNEBEL WERNER , MOELLMANN KYRA , ULRICH HEINRICH
Abstract: A microscope with a light source that produces an illumination light beam for evanescently illuminating a sample includes an adjustment mechanism with which the polarization of the illumination light beam may be changed.
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公开(公告)号:DE102005008196A1
公开(公告)日:2006-08-31
申请号:DE102005008196
申请日:2005-02-22
Applicant: LEICA MICROSYSTEMS
Inventor: RIEDMANN JUERGEN , KNEBEL WERNER , KUSCHEL LIOBA
IPC: G01N21/64
Abstract: A method for recognizing dark states during the spectroscopic or microscopic examination of fluorescent specimens includes varying an intensity distribution of excitation light by varying an excitation/illumination volume over a plurality of mutually independent measurements. A determination is made as to whether observed time constants change between the measurements. The existence of a dark state is inferred where the observed time constants are unchanged between the measurements.
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公开(公告)号:DE102005007756A1
公开(公告)日:2006-08-17
申请号:DE102005007756
申请日:2005-02-18
Applicant: LEICA MICROSYSTEMS
Inventor: KNEBEL WERNER
IPC: G02B21/06
Abstract: Scan microscope e.g. confocal scan microscope or stereo microscope comprises scanning device for an illumination or detection light beam and has a lens having optical axis. Scan microscope comprises of an illumination device for generating an illuminating light beam, which can be focused on the specimen, with illumination direction inclined to optical axis.
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公开(公告)号:DE102004016253B4
公开(公告)日:2006-02-23
申请号:DE102004016253
申请日:2004-04-02
Applicant: LEICA MICROSYSTEMS
Inventor: KNEBEL WERNER
IPC: G02B21/00
Abstract: A scanning microscope including a light source for generating an illumination light beam, a beam deflection apparatus for guiding solely the illumination light beam via an illumination light path over and/or through a sample, at least one objective for focusing the illumination light beam onto and/or into the sample, components for generating a manipulation illumination pattern, components for imaging the manipulation illumination pattern onto and/or into the sample via a manipulation light path, and a detection device that receives detected light emanating from the sample. The manipulation light path omits the beam deflection apparatus and is separate from the illumination light path.
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公开(公告)号:DE10359374A1
公开(公告)日:2005-07-14
申请号:DE10359374
申请日:2003-12-18
Applicant: LEICA MICROSYSTEMS
Inventor: ULRICH HEINRICH , KNEBEL WERNER
Abstract: A raster microscope sample investigation procedure combines continuous (11) and pulsed (5) light of the same wavelength to excite sample (41) photo activity for a FLIM (Fluorescent Lifetime Imaging Microscopy) examination and couples it into the microscope (37) through a beam splitter (19) and power adjusting (13) acousto optic (15) component. Independent claims are included for a raster microscope using the procedure with light guide carrying the combined light.
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