公开(公告)号：US20160097089A1

公开(公告)日：2016-04-07

申请号：US14823980

申请日：2015-08-11

Applicant: Fluidigm Canada Inc.

Inventor： Olga Ornatsky

IPC: C12Q1/68 ,  G01N33/68 ,  H01J49/00

Abstract: The invention relates to the use of inductively coupled plasma mass spectroscopy for cellular sample analysis. In some embodiments a method of performing mass spectroscopy analysis using an inductively coupled plasma mass spectroscopy system is provided. The method may include introducing a cellular sample comprising one or more cells or cellular particles into an inductively coupled plasma of the inductively coupled plasma mass spectroscopy system. The method may further include using the inductively coupled plasma mass spectroscopy system to assess the cellular sample by detecting and measuring one or more element tags in the cellular sample based on the element or isotopic compositions of the one or more element tags.

Abstract translation: 本发明涉及电感耦合等离子体质谱法用于细胞样品分析的用途。 在一些实施例中，提供了使用电感耦合等离子体质谱系统进行质谱分析的方法。 该方法可以包括将包含一个或多个细胞或细胞颗粒的细胞样品引入到电感耦合等离子体质谱系统的电感耦合等离子体中。 该方法还可以包括使用电感耦合等离子体质谱系统通过基于一个或多个元件标签的元件或同位素组成检测和测量细胞样品中的一个或多个元件标签来评估细胞样品。

公开(公告)号：US20160056031A1

公开(公告)日：2016-02-25

申请号：US14783791

申请日：2014-04-17

Applicant: FLUIDIGM CANADA INC.

Inventor： Alexandre Loboda

IPC: H01J49/04 ,  G01N15/10 ,  H01J49/10

CPC classification number: G01N15/10 ,  G01N2015/0065 ,  G01N2015/1006 ,  H01J49/0463 ,  H01J49/105 ,  H01J49/40

Abstract: The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.

Abstract translation: 本发明涉及使用激光烧蚀电感耦合等离子体质谱（LA-ICP-MS）分析样品的方法和装置。 本发明提供方法和装置，其中个体消融羽流被明显地捕获并转移到ICP，随后通过质谱法分析。

公开(公告)号：WO2022060624A1

公开(公告)日：2022-03-24

申请号：PCT/US2021/049667

申请日：2021-09-09

Applicant: FLUIDIGM CANADA INC.

Inventor： LOBODA, Alexander ,  JONG, Raymond ,  SULLIVAN, Michael ,  VOROBIEV, Serguei ,  ROTENBERG, Robert ,  STRATULATIV, Emil ,  VORONOV, Maxim ,  ARMSTRONG, Mark

IPC: H05H1/26 ,  H05H1/30 ,  H05H1/34 ,  G01N21/73

Abstract: Inductively coupled plasma (ICP) analyzers use an ICP torch to generate a plasma in which a sample is atomized an ionized. Analysis of the atomic ions can be performed by atomic analysis, such as mass spectrometry (MS) or atomic emission spectrometry (AES). Particle based ICP analysis includes analysis of particles such as cells, beads, or laser ablation plumes, by atomizing and ionizing particles in an ICP torch followed by atomic analysis. In mass cytometry, mass tags of particles are analyzed by mass spectrometry, such as by ICP-MS. Systems and methods of the subject application include one or more of: a demountable ICP torch holder assembly, an external ignition device; an ICP load coil comprising an annular fin, particle suspension sample introduction fluidics, and ICP analyzers thereof.

公开(公告)号：WO2021127435A1

公开(公告)日：2021-06-24

申请号：PCT/US2020/066012

申请日：2020-12-18

Applicant: FLUIDIGM CANADA INC.

Inventor： ALLO, Bedilu ,  LOU, Xudong ,  LIU, Peng ,  MAJONIS, Daniel ,  MAVROPOULOS, Anastasia ,  GHEIRATMAND, Ladan

IPC: C08F20/10 ,  C08F20/56 ,  C08F220/36 ,  C08F220/54 ,  C08F220/56

Abstract: Described herein are reagents and methods for improving signal in imaging mass cytometry. Aspects include mass tags with a large number of labeling atoms, chemical modifications to mass tags and additional reagents to reduce background and/or maintain target binding of mass tagged specific binding partners (SBPs), and schemes for associating a plurality of mass tags with a single SBP. As such, embodiments include any combination of one or more reagents and their use. The reagents, kits and methods herein may be used for mass cytometry, including imaging mass cytometry. In some aspects, reagents, kits or methods may be used for delivery of a large number of radioisotopes to a target analyte, for example for therapeutic use or radiometric detection. In certain aspects, only non-radioactive isotopes may be used for mass cytometry.

公开(公告)号：WO2019210233A1

公开(公告)日：2019-10-31

申请号：PCT/US2019/029443

申请日：2019-04-26

Applicant: FLUIDIGM CANADA INC. ,  FLUIDIGM CORPORATION ,  ZABINYAKOV, Nick

Inventor： ZABINYAKOV, Nick ,  LOBODA, Alexander V. ,  ORNATSKY, Olga ,  BARANOV, Vladimir

IPC: C12M1/00 ,  C12M1/34 ,  G01N35/00 ,  G01N35/10 ,  H01J49/00 ,  H01J49/02

Abstract: Embodiments of the present invention relate to reagents and their use for elemental imaging mass spectrometry of biological samples. The embodiments comprising methods for quantifying one or more analytes within a sample, comprising the steps of: (a) providing the sample, wherein the one or more analytes are immobilized to a sample carrier, wherein the sample has been labelled with one or more mass tags comprising one or more labelling atoms, (b) performing mass cytometry on the sample to determine the level of the one or more labelling atoms, wherein the level of the one or more labelling atoms corresponds to the copy number of the one or more analytes.

公开(公告)号：WO2016077770A3

公开(公告)日：2016-10-13

申请号：PCT/US2015060706

申请日：2015-11-13

Applicant: FLUIDIGM CANADA INC

Inventor： LOBODA ALEXANDER V

IPC: G01N33/48 ,  H01J49/26

CPC classification number: G01N33/6848 ,  G01N33/58 ,  G01N2458/15

Abstract: Methods of imaging a biological sample by mass cytometry using molecular tagging are disclosed.

Abstract translation: 公开了通过使用分子标记的质谱法对生物样品进行成像的方法。

公开(公告)号：WO2016077770A8

公开(公告)日：2016-09-01

申请号：PCT/US2015060706

申请日：2015-11-13

Applicant: FLUIDIGM CANADA INC

Inventor： LOBODA ALEXANDER V

IPC: G01N33/68

CPC classification number: G01N33/6848 ,  G01N33/58 ,  G01N2458/15

Abstract: Methods of imaging a biological sample by mass cytometry using molecular tagging are disclosed.

Abstract translation: 公开了使用分子标记通过质量细胞计量术对生物样品进行成像的方法。

公开(公告)号：WO2016077770A2

公开(公告)日：2016-05-19

申请号：PCT/US2015/060706

申请日：2015-11-13

Applicant: FLUIDIGM CANADA INC. ,  FLUIDIGM CORPORATION

Inventor： LOBODA, Alexander V.

IPC: G01N33/68

CPC classification number: G01N33/6848 ,  G01N33/58 ,  G01N2458/15

Abstract: Methods of imaging a biological sample by mass cytometry using molecular tagging are disclosed.

Abstract translation: 公开了使用分子标签通过质量细胞计量术对生物样品进行成像的方法。

公开(公告)号：WO2014194417A1

公开(公告)日：2014-12-11

申请号：PCT/CA2014/050496

申请日：2014-05-28

Applicant: FLUIDIGM CANADA INC.

Inventor： TANNER, Scott

IPC: H01J49/40 ,  H01J49/06 ,  H03M1/12

CPC classification number: H01J49/40 ,  H01J49/025

Abstract: A mass spectrometer of the type useful in mass cytometry includes an ion detector. A digitizing system for converting analog signals from the ion detector includes two analog-to-digital converters. The analog-to-digital converters are configured to provide an increased dynamic range for a targeted period while limiting the amount of data generated.

Abstract translation: 可用于质量细胞计数的质谱仪包括离子检测器。 用于转换离子检测器的模拟信号的数字化系统包括两个模拟 - 数字转换器。 模数转换器被配置为为目标周期提供增加的动态范围，同时限制所生成的数据量。

公开(公告)号：WO2014190429A1

公开(公告)日：2014-12-04

申请号：PCT/CA2014/050474

申请日：2014-05-22

Applicant: FLUIDIGM CANADA INC.

Inventor： BANDURA, Dmitry ,  BARANOV, Vladimir ,  LOBODA, Alexander ,  ORNATSKY, Olga ,  TANNER, Scott

IPC: G01N27/62 ,  G01N33/483

CPC classification number: G01N33/54366 ,  G01N15/1031 ,  G01N15/1404 ,  G01N15/1459 ,  G01N27/622 ,  G01N27/624 ,  G01N33/483 ,  G01N33/532 ,  G01N33/54313 ,  G01N2015/1006 ,  G01N2458/15

Abstract: The invention relates to methods and devices for molecular cytometry. The molecular cytometer is for use in the analysis of molecular tag labeled particles. In the molecular cytometer, volatile molecular tags attached to the particles, such as cells, are released as groups of molecular tags and ionized by the method of soft ionization to produce corresponding groups of molecular ions. The molecular cytometer has two detectors, one to detect the presence of the groups of molecular ions and the other to detect mobility separated molecular ions. The mobility separated molecular ions are synchronized to correspond with the groups of molecular ions.

Abstract translation: 本发明涉及用于分子细胞计数的方法和装置。 分子光谱仪用于分子标签标记颗粒的分析。 在分子细胞仪中，附着于粒子（如细胞）的挥发性分子标记作为分子标签的基团释放，并通过软电离方法离子化以产生相应的分子离子组。 分子细胞仪有两个检测器，一个用于检测分子离子组的存在，另一个用于检测迁移率分离的分子离子。 迁移率分离的分子离子被同步以对应于分子离子的组。