公开(公告)号：KR1020090013599A

公开(公告)日：2009-02-05

申请号：KR1020070077854

申请日：2007-08-02

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 김종만 ,  김재명 ,  이희수 ,  장금찬 ,  박신영 ,  조윤상 ,  이명헌 ,  윤소미 ,  박중원 ,  지순옥 ,  정석찬

IPC: A61K39/02 ,  A61P37/02 ,  A61K39/085

CPC classification number: A61K39/05 ,  C12R1/47 ,  Y10S435/887 ,  Y10S514/885

Abstract: An immunostimulant containing the lystic extract of Streptomyces albus is provided to reinforce the functions of various immune cells having the major role in the disease defense, thereby increasing the specific and nonspecific resistance to the disease infection of animals. The immunostimulant contains the lystic extract of Streptomyces albus which is prepared by cultivating Streptomyces albus in yeast malt extract agar broth at 26 deg. C for 72 hours, centrifuging the cultured fluid at 3000Xg for 30 minutes and collecting it, rinsing the collected pellet with phosphate buffered saline(PBS) of pH 7.2 and suspending the rinsed pellet in PBS, adding 5% of TNT solution containing 50mM of Tris-HCl, 0.15M of NaCl and 4% Triton x-114 as solubilizing agent into the Streptomyces albus suspension, stirring the mixture at room temperature for 2-3 days to solubilize the Streptomyces albus, and regulating pH of the solution to 6.5-7.2, and drying the solution in the thermostatic chamber.

Abstract translation: 提供含有链霉菌（Streptomyces albus）的提取物的免疫刺激剂，以增强在疾病防御中具有主要作用的各种免疫细胞的功能，从而增加对动物疾病感染的特异性和非特异性抗性。 免疫刺激剂含有通过在26度的酵母麦芽提取物琼脂培养基中培养链霉菌（Streptomyces albus）而制备的链霉菌链霉菌的提取物。 培养72小时，将培养液以3000Xg离心30分钟并收集，用pH7.2的磷酸盐缓冲盐水（PBS）冲洗收集的沉淀物，并将漂洗的沉淀悬浮于PBS中，加入5％含有50mM Tris -HCl，0.15M NaCl和4％Triton x-114作为增溶剂加入到Streptomyces albus悬浮液中，在室温下搅拌混合物2-3天以溶解链霉菌，并将溶液的pH调节至6.5-7.2 ，并干燥恒温室中的溶液。

公开(公告)号：KR1020080093488A

公开(公告)日：2008-10-22

申请号：KR1020070037227

申请日：2007-04-17

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 김종만 ,  이희수 ,  김재명 ,  임숙경 ,  박신영 ,  장금찬 ,  임춘태 ,  정석찬

IPC: A61K36/233 ,  A61P37/00

Abstract: An extract of Bupleuri Radix is provided to increase various activities of main immunocytes of an animal such as granulocyte and macrophage and induce strong defence capability on attach inoculation of pathogenic bacteria. A composition comprising the same extract is provided to be used as an animal medicine such as an animal immuno-enhancing agent and a vaccine adjuvant and a feed additive for treating and preventing animal diseases efficiently. A method for purifying an extract of Bupleuri Radix comprises the steps of: (a) heat-treating the Bupleuri Radix with hot water at a temperature of 80-120 deg.C for 2-3 hours to obtain a crude extract; (b) concentrating the crude extract; and (c) filtering the concentrate to obtain a polysaccharide fraction by filtering paper and microorganism filtering. An extract of Bupleuri Radix purified by the method is a Bupleuri Radix polysaccharide, of which sugar is a glycoprotein including a protein. A composition for increasing immunity comprises the extract of Bupleuri Radix as an active ingredient and a pharmaceutically acceptable carrier or an excipient. An animal immunity enhancing agent, a vaccine adjuvant, or a feed additive for an animal comprises the composition for increasing immunity as an active ingredient.

Abstract translation: 提供柴胡提取物，以增加动物如粒细胞和巨噬细胞的主要免疫细胞的各种活性，并在附着接种病原菌时诱导强大的防御能力。 提供包含相同提取物的组合物用作动物药物，例如动物免疫增强剂和疫苗佐剂和用于有效治疗和预防动物疾病的饲料添加剂。 纯化柴胡提取物的方法包括以下步骤：（a）在80-120℃的温度下用热水热处理柴胡，2-3小时，得到粗提物; （b）浓缩粗提物; 和（c）过滤浓缩物以通过滤纸和微生物过滤获得多糖级分。 通过该方法纯化的柴胡提取物是一种柴胡甘草多糖，其中糖是包含蛋白质的糖蛋白。 用于增加免疫力的组合物包含作为活性成分的柴胡提取物和药学上可接受的载体或赋形剂。 动物免疫增强剂，疫苗佐剂或用于动物的饲料添加剂包括用于增加免疫力作为活性成分的组合物。

公开(公告)号：KR1020080084384A

公开(公告)日：2008-09-19

申请号：KR1020070026062

申请日：2007-03-16

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 이희수 ,  김종만 ,  임숙경 ,  김재명 ,  박신영 ,  임춘태 ,  장금찬 ,  정석찬

IPC: A61K36/238 ,  A61P37/02

Abstract: An extract of Saposhnikovia root is provided to improve immune response by increasing activities of neutrophil, macrophage and lymphocyte, production of cytokines and non-specific protection against pathogenic bacteria infection in animals, so that it is useful as immunomodulator and immunoadjuvant. An extract of Saposhnikovia root for improving immune response in animals is prepared by heating Saposhnikovia root in water, and administered into an animal, especially domestic animals including cow, pig and chicken in the amount of 10-20 mg/kg per day through oral administration, intravenous injection, intramuscular injection or subcutaneous injection.

Abstract translation: 提供Saposhnikovia根的提取物，通过增加嗜中性粒细胞，巨噬细胞和淋巴细胞的活性，产生细胞因子和非特异性保护免受动物中的致病细菌感染来改善免疫应答，从而可用作免疫调节剂和免疫佐剂。 通过在水中加热山茱萸碱来制备Saposhnikovia根的提取物，并通过口服给药至每天10-20mg / kg的动物，特别是包括牛，猪和鸡的家畜，其量为10-20mg / kg ，静脉注射，肌内注射或皮下注射。