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公开(公告)号：KR100915898B1
公开(公告)日：2009-09-07
申请号：KR1020070017534
申请日：2007-02-21
Applicant: 한국과학기술연구원
IPC: C12Q1/68
Abstract: 본 발명은 다환 방향족 탄화수소류 중의 하나인 3-메틸콜란트렌(3-methylcholanthrene)에 대한 노출 여부 진단용 바이오마커 및 이를 이용한 진단 방법에 관한 것으로, 구체적으로 3-메틸콜란트렌에 특이적으로 유전자 발현이 증가 또는 감소하는 바이오마커 및 이를 이용한 3-메틸콜란트렌에 대한 노출 여부를 진단하는 방법에 관한 것이며, 본 발명의 바이오마커는 DNA 마이크로어레이 칩을 통하여 선별된 반응 유전자들을 바이오마커로 이용하여 환경 시료에서 3-메틸콜란트렌의 오염을 모니터링 및 판정하는데 유용하게 사용될 수 있으며, 3-메틸콜란트렌에 의해 유발되는 독성 작용 기작을 규명하는 도구로 이용될 수 있다.
3-메틸콜란트렌, 다환 방향족 탄화수소, 마이크로어레이 칩-
公开(公告)号：KR1020090050720A
公开(公告)日：2009-05-20
申请号：KR1020070117317
申请日：2007-11-16
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6883 , C12Q1/6837 , C12Q2563/107 , C12Q2600/142
Abstract: 본 발명은 최기형성 및 부작용 유발 약물 검색용 바이오마커 및 이를 이용한 검색 방법에 관한 것으로, 구체적으로 다양한 최기형성 유발 약물에 공통적으로 유전자 발현이 증가 또는 감소하는 바이오마커 및 이를 이용한 최기형성 및 부작용 유발 약물 검색 방법에 관한 것이다. 본 발명의 바이오마커는 DNA 마이크로어레이 칩을 통하여 선별된 반응 유전자들을 바이오마커로 이용하여 새로운 최기형성 및 부작용의 위험성을 지닌 약물 또는 화학물질을 모니터링 및 판정하는데 유용하게 사용될 수 있으며, 최기형성을 일으키는 작용 기작을 규명하는 도구로 이용될 수 있다.
최기형성 약물, 마이크로어레이-
公开(公告)号：KR100896934B1
公开(公告)日：2009-05-14
申请号：KR1020030072652
申请日：2003-10-17
Applicant: 한국과학기술연구원
IPC: C12Q1/68
Abstract: 본 발명은 환경호르몬 검색용 cDNA 칩에 관한 것으로서, 보다 구체적으로는 하우스키핑 유전자, 약물 대사 관련 유전자, 에스트로겐 반응 유전자, 에스트로겐에 의해 발현이 증가하는 유전자, 안드로젠 반응 유전자, 안드로젠에 의해 조절되는 유전자, 타이로이드 호르몬 반응 유전자, 성호르몬 신호 전달 관련 유전자, 성호르몬 조절 관련 유전자, 내분비계 신호 전달 관련 유전자, 내분비계 조절 관련 유전자, c-fos 관련 유전자, c-myc 관련 유전자, pS2 관련 유전자 및 오버래핑 유전자로 구성된 군에서 6개 이상의 유전자를 포함하며, 상기 유전자가 고체상 기질에 일정 간격으로 배열하여 집적되는 것을 특징으로 하는 환경 호르몬 검색용 cDNA 칩 및 상기 cDNA 칩을 이용하여 환경 호르몬을 검색하고, 환경 호르몬 관련 유전자를 검색하는 방법에 관한 것이다. 본 발명의 cDNA 칩을 이용하면 호르몬에 의해 발현 조절되는 유전자를 손쉽게 분석할 수 있어 환경 호르몬을 검색하는데 유용하다.
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Patent Agency Ranking
公开(公告)号：KR1020090039354A
公开(公告)日：2009-04-22
申请号：KR1020070104948
申请日：2007-10-18
Applicant: 한국과학기술연구원
IPC: C12Q1/68
CPC classification number: C12Q1/6883 , C12Q1/6827 , C12Q1/6886 , C40B40/08
Abstract: A biomarker for detecting exposure to benzo[k]fluoranthene is provided to monitor and detect benzo[k]fluoranthene contamination in environmental sample and examine toxicity mechanism caused by benzo[k]fluoranthene. A biomarker for detecting exposure to benzo[k]fluoranthene comprises a gene whose expression is increased or decreased by the exposure to the benzo[k]fluoranthene. A DNA microarray chip is integrated with oligo-nucleotide comprising entire or a part of biomarker gene sequence or its complementary sequence. A method for detecting the exposure to the benzo[k]fluoranthene comprises: a step of collecting cell from a target; a step of RNA isolation from the collected cell as a experimental group and human cell untreated as a control group; a step of synthesizing the RNA of experimental group and control group with cDNA and probing with different fluorescence; a step of hybridizing the fluorescence attached cDNA with DNA microarray chip; a step of analyzing the DNA microarray chip; and a step of comparing expression level of biomarker with the control group and judging exposure to benzo[k]fluoranthene.
Abstract translation: 提供了一种用于检测苯并[k]荧蒽暴露的生物标志物，用于监测和检测环境样品中的苯并[k]荧蒽污染物，并检测苯并[k]荧蒽产生的毒性机制。 用于检测暴露于苯并[k]荧蒽的生物标志物包括通过暴露于苯并[k]荧蒽而表达增加或降低的基因。 将DNA微阵列芯片与包含全部或部分生物标记基因序列或其互补序列的寡核苷酸整合。 检测对苯并[k]荧蒽的暴露的方法包括：从目标物收集细胞的步骤; 作为实验组从收集的细胞中分离RNA的步骤和未处理作为对照组的人细胞; 用cDNA合成实验组和对照组的RNA并用不同荧光探测的步骤; 将荧光连接的cDNA与DNA微阵列芯片杂交的步骤; 分析DNA微阵列芯片的步骤; 比较生物标志物与对照组的表达水平并判断苯并[k]荧蒽的暴露的步骤。
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公开(公告)号：KR1020090019501A
公开(公告)日：2009-02-25
申请号：KR1020070083990
申请日：2007-08-21
Applicant: 한국과학기술연구원
IPC: C12Q1/68
CPC classification number: C12Q1/6876 , C12Q1/6837
Abstract: Marker genes for screening of drug inducing pulmonary toxicity are provided to monitor the drug or chemical substance having the risk of new lung toxicity or determine the risk of the drug or chemical substance, and identify the mechanism causing lung toxicity caused by carbamazepine and side effects. The marker gene for screening of drug inducing pulmonary toxicity comprises the gene selected from the group consisting of the asparagine synthetase gene(Genebank NM 133436 B), the brain-derived neurotrophic factor opposite strand gene(Genebank NM 170735 B), the cyclin D2 gene(Genebank NM 001759 B), the gene(Genebank NM 005318 B)(H1 histone family, member 0), gene(Genebank NM 000575 Bs)(Interleukin 1, alpha), and the gene(Genebank NM 000600 B)[Interleukin 6(interferon, beta 2)].
Abstract translation: 提供用于筛选引起肺部毒性的药物的标记基因，用于监测具有新肺毒性风险的药物或化学物质，或确定药物或化学物质的风险，并确定导致卡马西平引起的肺毒性和副作用的机制。 用于筛选引起肺部毒性的药物的标记基因包括选自天冬酰胺合成酶基因（Genebank NM133436B），脑源性神经营养因子相反链基因（Genebank NM 170735B），细胞周期蛋白D2基因 （Genebank NM 001759B），基因（Genebank NM 005318B）（H1组蛋白家族，成员0），基因（Genebank NM 000575Bs）（白细胞介素1，α）和基因（Genebank NM 000600B）[白细胞介素6 （干扰素，β2）]。
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公开(公告)号：KR1020080077834A
公开(公告)日：2008-08-26
申请号：KR1020070017534
申请日：2007-02-21
Applicant: 한국과학기술연구원
IPC: C12Q1/68
CPC classification number: C12Q1/6837 , C12Q2563/107 , C12Q2600/158
Abstract: A biomarker selected through a DNA microarray chip is provided to be usefully used to monitor and determine 3-methylcholanthrene contamination and be used as a tool for identifying a toxicity mechanism induced by the 3-methylcholanthrene. A biomarker for diagnosing exposure to 3-methylcholanthrene includes a gene selected from the group consisting of Genebank NOs. NM_000499, NM_203347, NM_006227, NM_018837, NM_001769, NM_001402, NM_170693, NM_021599, NM_001017973, NM_032324, NM_198194, NM_016368, NM_030569, NM_001748, NM_012467, NM_001917, NM_015920, NM_001009820, NM_014010, NM_152640, NM_001019, NM_002842, NM_001004, NM_006929, NM_012190, NM_032872, NM_000480, NM_000476, NM_018161, NM_021063, NM_001005749, NM_005319, NM_001015053, NM_003528, NM_002778, NM_021058, NM_003524, NM_003519, NM_033445, NM_005572, NM_003520, NM_003527, NM_175055, NM_000596, NM_004864, NM_201525, NM_014624, NM_175744, NM_005620, NM_006018, NM_005310, NM_002926, NM_021913, NM_002194, BC064982, NM_001005339, NM_021077, NM_004881, NM_018494, NM_033285, NM_001197, NM_001540, NM_003820, NM_147780, NM_001003940, NM_000389, NM_016639, NM_000043, NM_002307, NM_021603, NM_004925, NM_000014, NM_022449, NM_005855, NM_006868, NM_032493, NM_007097, NM_005697, NM_004073, NM_182795, NM_005072, NM_144606, NM_014059, NM_002754, NM_003254, NM_001553, NM_003255, NM_013376, NM_000107, NM_004433, NM_021969, NM_023039, NM_080875, NM_002166, NM_001421, NM_153813, U68019, NM_015655, NM_031918, NM_001554, NM_003960, NM_032965, NM_201397, NM_005064, NM_005345, NM_001531, NM_006404, NM_002119, NM_005101, NM_006426, NM_020728, NM_016441, NM_203391, NM_006775, NM_052937, NM_003932, NM_030979, NM_032549, NM_007195, NM_002139, C033021, NM_016093, CR611166, NM_017437, NM_033114, NM_000236, NM_174936, NM_004375, NM_004685, NM_052965, NM_201278, NM_003093, AV757313, NM_014791, BX537987, BC041925, BC032643, NM_016058, NM_181886, NM_016271, AK021676, NM_015352, NM_016304, NM_020995, NM_003017, W04231, NM_006546, NM_212554, BC049823, NM_006937, NM_012331, NM_005875, NM_032906, NM_002847, NM_000947, NM_181716, NM_004681, NM_000982, T07777, NM_016652, NM_000986, NM_139207, AK001406, NM_145649, NM_020236, NM_001017430, NM_031157, NM_018981, NM_018291, NM_018048, NM_012207, NM_000028, NM_014321, NM_015423, NM_001034, AB032990, NM_000791, NM_199040, NM_006886, NM_004457, NM_007295, NM_006111, NM_001443, NM_000016, NM_001809, AL833119, NM_006493, NM_138271, NM_006136, CR615278, NM_024704, NM_003011, NM_203401, CR749233, AF277624, NM_013282, NM_198893, NM_013361, NM_002129, NM_002128, NM_003429, NM_003423, NM_016220, NM_022103, NM_021269, NM_016649, NM_199132, AK098175, NM_198381, NM_138330, NM_030824, NM_003441, NM_001001415, AK128731, NM_203282, CR627133, NM_007139, NM_178558, NM_024498, NM_003430, NM_003410, NM_178549, NM_152601, NM_024629, BC015987, NM_145233, NM_031942, NM_133473, NM_005655, NM_021994, NM_025189, NM_001008390, NM_024087, NM_032828, NM_006980, NM_024561, NM_173531, NM_003864, NM_012345, NM_003079, NM_006193, NM_003599, NM_006311, NM_020675, NM_018492, NM_145697, NM_006101, NM_002358, NM_006716, NM_003318, NM_018131, NM_012177, NM_013277, NM_018136, NM_014750, NM_016343, NM_017489, NM_006461, NM_001790, NM_004064, NM_080668, NM_021211, NM_001274, , NM_001005414, NM_144710, NM_004336, NM_005496, NM_181803, NM_018101, AF147440, NM_005030, NM_022346, NM_019084, NM_005504, NM_004523, NM_004354, NM_001237, NM_032626, NM_021930, NM_018685, NM_016195, NM_001827, NM_138555, NM_031966, NM_005915, NM_014751, NM_003981, NM_001826, NM_198219, NM_014881, NM_001255, NM_016359, AF085846, NM_001656, NM_145307, NM_080651, NM_003714, NM_018098, NM_004586, NM_002317, NM_031296, NM_014736, XM_292197, NM_016513, NM_001331, AB032991, NM_004232, NM_003472, NM_012334, NM_012425, NM_002140, NM_005271, NM_145203, BU679059, NM_006305, NM_020824, NM_012120, NM_012089, NM_021977, NM_015171, NM_016467, AA484677, BC035622, NM_002520, NM_014043, NM_003133, NM_013322, NM_005733, NM_014322, NM_020401, NM_138285, NM_001786, NM_001067, NM_001012271, NM_024854, NM_018204, NM_021999, NM_005400, NM_148957, NM_001006, NM_006437, NM_024055, NM_000582, NM_016951, NM_002737, NM_004836, NM_020686, NM_004134, NM_053039, NM_007195, NM_005256, NM_003368, NM_001018115, AI281523, NM_004075, NM_002389, NM_000584, NM_001801, NM_000715, NM_144503, NM_145697, and NM_001010914.
Abstract translation: 提供通过DNA微阵列芯片选择的生物标志物，以有效地用于监测和测定3-甲基胆蒽污染物，并用作识别3-甲基胆蒽诱导的毒性机制的工具。 用于诊断3-甲基胆蒽暴露的生物标志物包括选自Genebank NOs的基因。 NM_000499，NM_203347，NM_006227，NM_018837，NM_001769，NM_001402，NM_170693，NM_021599，NM_001017973，NM_032324，NM_198194，NM_016368，NM_030569，NM_001748，NM_012467，NM_001917，NM_015920，NM_001009820，NM_014010，NM_152640，NM_001019，NM_002842，NM_001004，NM_006929，NM_012190， NM_032872，NM_000480，NM_000476，NM_018161，NM_021063，NM_001005749，NM_005319，NM_001015053，NM_003528，NM_002778，NM_021058，NM_003524，NM_003519，NM_033445，NM_005572，NM_003520，NM_003527，NM_175055，NM_000596，NM_004864，NM_201525，NM_014624，NM_175744，NM_005620，NM_006018， NM_005310，NM_002926，NM_021913，NM_002194，BC064982，NM_001005339，NM_021077，NM_004881，NM_018494，NM_033285，NM_001197，NM_001540，NM_003820，NM_147780，NM_001003940，NM_000389，NM_016639，NM_000043，NM_002307，NM_021603，NM_004925，NM_000014，NM_022449，NM_005855，NM_006868， NM_032493，NM_007097，NM_005697，NM_004073，NM_182795，NM_005072，NM_144606，NM_014059，NM_002754，NM_003254，NM_001553，NM_003255，NM_013376，NM_000107，NM_0 04433，NM_021969，NM_023039，NM_080875，NM_002166，NM_001421，NM_153813，U68019，NM_015655，NM_031918，NM_001554，NM_003960，NM_032965，NM_201397，NM_005064，NM_005345，NM_001531，NM_006404，NM_002119，NM_005101，NM_006426，NM_020728，NM_016441，NM_203391，NM_006775， NM_052937，NM_003932，NM_030979，NM_003932，NM_030979，NM_003932，NM_030979，NM_032549，NM_007195，NM_002139，CO033021，NM_016093，CR611166，NM_017437，NM_033114，NM_000236，NM_174936，NM_004375，NM_004685，NM_052965，NM_201278，NM_003093，AV757313，NM_014791，BX537987，BC041925，BC032643，NM_016058，NM_181886， NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271， NM_020236，NM_001017430，NM_031157，NM_018981，NM_018291，NM_018048，NM_012207，NM_000028，NM_014321，NM_015423，NM_001034，AB032990，NM_000791，NM_199040，NM_006886，NM_004457，NM_007295，NM_006111 ，NM_001443，NM_000016，NM_001809，AL833119，NM_006493，NM_138271，NM_006136，CR615278，NM_024704，NM_003011，NM_203401，CR749233，AF277624，NM_013282，NM_198893，NM_013361，NM_002129，NM_002128，NM_003429，NM_003423，NM_016220，NM_022103，NM_021269，NM_016649，NM_199132 ，AK098175，NM_198381，NM_138330，NM_030824，NM_003441，NM_001001415，AK128731，NM_203282，CR627133，NM_007139，NM_178558，NM_024498，NM_003430，NM_003410，NM_178549，NM_152601，NM_024629，BC015987，NM_145233，NM_031942，NM_133473，NM_005655，NM_021994，NM_025189，NM_001008390 ，NM_024087，NM_032828，NM_006980，NM_024561，NM_173531，NM_003864，NM_012345，NM_003079，NM_006193，NM_003599，NM_006311，NM_020675，NM_018492，NM_145697，NM_006101，NM_002358，NM_006716，NM_003318，NM_018131，NM_012177，NM_013277，NM_018136，NM_014750，NM_016343，NM_017489 ，NM_006461，NM_001790，NM_004064，NM_080668，NM_021211，NM_001274，NM_001005414，NM_144710，NM_004336，NM_005496，NM_181803，NM_018101，AF147440，NM_005030，NM_022346，NM_019 084，NM_005504，NM_004523，NM_005504，NM_004523，NM_005504，NM_004523，NM_005504，NM_004523，NM_005504，NM_004523，NM_005504，NM_004523，NM_005504，NM_004523，NM_005504，NM_004523，NM_005504， NM_018098，NM_004586，NM_002317，NM_031296，NM_014736，XM_292197，NM_016513，NM_001331，AB032991，NM_004232，NM_003472，NM_012334，NM_012425，NM_002140，NM_005271，NM_145203，BU679059，NM_006305，NM_020824，NM_012120，NM_012089，NM_021977，NM_015171，NM_016467，AA484677， BC035622，NM_002520，NM_014043，NM_003133，NM_013322，NM_005733，NM_014322，NM_020401，NM_138285，NM_001786，NM_001067，NM_001012271，NM_024854，NM_018204，NM_021999，NM_005400，NM_148957，NM_001006，NM_006437，NM_024055，NM_000582，NM_016951，NM_002737，NM_004836，NM_020686， NM_004134，NM_053039，NM_007195，NM_005256，NM_003368，NM_001018115，AI281523，NM_004075，NM_002389，NM_000584，NM_001801，NM_000715，NM_144503，NM_145697和NM_001010914中。
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公开(公告)号：KR1020080045987A
公开(公告)日：2008-05-26
申请号：KR1020060115345
申请日：2006-11-21
Applicant: 한국과학기술연구원
IPC: C12Q1/68
CPC classification number: C12Q1/6837 , C12N15/63 , C12Q2563/107
Abstract: A biomarker is provided to use reaction genes selected from a DNA microarray chip for monitoring and determining teratogenicity and side effects of valproic acid during treatment of epilepsy and manic depressive. A biomarker for screening and diagnosing teratogenicity of valproic acid includes a gene selected from the group consisting of Genebank NM_001465, Genebank NM_005953, Genebank NM_005931, Genebank NM_021626, Genebank NM_145058, Genebank NM_003311, Genebank AF316855, Genebank NM_006705, Genebank NM_022157, Genebank NM_001005360, Genebank NM_006290, Genebank NM_002305, Genebank BC051787, Genebank NM_001446, Genebank NM_001386, Genebank NM_015103, Genebank NM_002165, Genebank NM_002166, Genebank NM_002167, Genebank NM_152243, Genebank NM_012234, Genebank NM_002389, Genebank AB044807, Genebank NM_017512, Genebank NM_018269, Genebank NM_004403, Genebank AK094629, Genebank NM_005139, Genebank NM_199286, Genebank NM_024091, Genebank NM_005871, Genebank NM_018147, Genebank NM_006265, Genebank NM_020371, Genebank NM_004331, Genebank NM_000254, Genebank NM_205842, Genebank NM_006522, Genebank AA399656, Genebank NM_004010, and Genebank NM_016441. A DNA microarray chip for screening the valproic acid is characterized in that an oligonucleotide including a total or a portion of the biomarker gene sequence or a complimentary strand molecule thereof is integrated. A method for screening and diagnosing the teratogenicity of the valproic acid comprises the steps of: (a) treating human choriocarcinoma cells with a compound to be tested; (b) isolating RNA respectively from the test group cells treated with the compound to be tested and control group cells; (c) labelling the test group and the control group, respectively, with a fluorescent material different from each other with synthesizing the RNA of the test group and the control group into cDNA; (d) hybridizing each the cDNA(s) labelled with the fluorescent material into the DNA microarray chip; (e) analyzing the reacted DNA microarray chip; and (f) comparing the expression degree of the biomarker from the data analyzed by the step(e) with that of the control group.
Abstract translation: 提供生物标志物以使用选自DNA微阵列芯片的反应基因来监测和测定治疗癫痫和躁狂抑郁症期间丙戊酸的致畸性和副作用。 用于筛选和诊断丙戊酸致畸性的生物标志物包括选自Genebank NM_001465，Genebank NM_005953，Genebank NM_005931，Genebank NM_021626，Genebank NM_145058，Genebank NM_003311，Genebank AF316855，Genebank NM_006705，Genebank NM_022157，Genebank NM_001005360，Genebank NM-006290，Genebank NM_002305，Genebank NM_002305，Genebank NM_002305，Genebank NM_002305，Genebank NM_001386，Genebank NM_001386，Genebank NM_015103，Genebank NM_002165，Genebank NM_002166，Genebank NM_002167，Genebank NM_152243，Genebank NM_012234，Genebank NM_002389，Genebank NM018807，Genebank NM_017512，Genebank NM_018269，Genebank NM_004403， Genebank NM_005139，Genebank NM_199286，Genebank NM_024091，Genebank NM_005871，Genebank NM_018147，Genebank NM_006265，Genebank NM_020371，Genebank NM_004331，Genebank NM_000254，Genebank NM_205842，Genebank NM_006522，Genebank AA399656，Genebank NM_004010和Genebank NM_016441。 用于筛选丙戊酸的DNA微阵列芯片的特征在于整合了包含生物标记基因序列的全部或部分或其互补链分子的寡核苷酸。 筛选和诊断丙戊酸致畸性的方法包括以下步骤：（a）用待测化合物处理人绒毛膜癌细胞; （b）分别从用待测化合物和对照组细胞处理的试验组细胞中分离RNA; （c）分别用不同的荧光材料标记试验组和对照组，将试验组和对照组的RNA合成cDNA; （d）将用荧光物质标记的每个cDNA与DNA微阵列芯片杂交; （e）分析反应的DNA微阵列芯片; 和（f）将来自步骤（e）分析的数据与对照组的表达程度进行比较。
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