公开(公告)号：DE10359660A1

公开(公告)日：2005-07-28

申请号：DE10359660

申请日：2003-12-18

Applicant: BASF AG

Inventor： KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: C12N9/02 ,  C12N15/77 ,  C12N15/63 ,  C12N15/67 ,  C12N1/00 ,  C12P1/00 ,  C12P13/08 ,  C12P13/12

Abstract: Use of a nucleic acid (I) with promoter activity for transcribing genes, where (I) is (a) a 173 nucleotide sequence (SEQ ID No.:1); (b) a variant of (SEQ ID No.:1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; (c) a sequence that hybridizes to (SEQ ID No.:1) under stringent conditions; or (d) a functionally equivalent fragment of (a)-(c), is new. Independent claims are also included for: (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (SEQ ID No.:1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (SEQ ID No.:1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence gaaagga (SEQ ID No.:44) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequences tgcaat (SEQ ID No.:42) and tatcatt (SEQ ID No.:43) as -10 regionS for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (SEQ ID No.:42) - (SEQ ID No.:44).

公开(公告)号：CA2549171A1

公开(公告)日：2005-06-30

申请号：CA2549171

申请日：2004-12-16

Applicant: BASF AG

Inventor： KLOPPROGGE CORINNA ,  ZELDER OSKAR ,  HAEFNER STEFAN ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG

IPC: C12N15/77 ,  C12N1/21 ,  C12N9/02

Abstract: The invention relates to the use of nucleic acid sequences for regulating ge ne transcription and expression, said novel promoters and expression units, methods for modifying or inducing the gene transcription rate and/or expression rate, expression cassettes containing said expression units, genetically modified microorganisms having a modified or induced transcripti on rate and/or expression rate, and methods for producing biosynthetic products by cultivating said genetically modified microorganisms.

公开(公告)号：CA2548349A1

公开(公告)日：2005-06-30

申请号：CA2548349

申请日：2004-12-15

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  KROEGER BURKHARD ,  HAEFNER STEFAN ,  SCHROEDER HARTWIG ,  KLOPPROGGE CORINNA

IPC: C12N15/77 ,  C07K14/34 ,  C12N20060101 ,  C12N1/00 ,  C12N1/21 ,  C12N15/63 ,  C12N15/67 ,  C12P1/00 ,  C12P13/08 ,  C12P13/12

Abstract: The invention relates to the use of nucleic acid sequences for regulating ge ne transcription and expression, said novel promoters and expression units, methods for modifying or inducing the gene transcription rate and/or expression rate, expression cassettes containing said expression units, genetically modified microorganisms having a modified or induced transcripti on rate and/or expression rate, and methods for producing biosynthetic products by cultivating said genetically modified microorganisms.

公开(公告)号：CA2548125A1

公开(公告)日：2005-06-30

申请号：CA2548125

申请日：2004-12-17

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  KIEFER PATRICK ,  KROGER BURKHARD ,  KLOPPROGGE CORINNA ,  HAEFNER STEFAN ,  WITTMANN CHRISTOPH ,  SCHROEDER HARTWIG ,  HEINZLE ELMAR

IPC: C12P13/08 ,  C12N1/21 ,  C12N9/04 ,  C12N15/53

Abstract: The present invention features methods of increasing the production of a fin e chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., lactate dehydrogenase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of the expression of lactate dehydrogenase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides method s for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

公开(公告)号：AU2004299259A1

公开(公告)日：2005-06-30

申请号：AU2004299259

申请日：2004-12-16

Applicant: BASF AG

Inventor： HAEFNER STEFAN ,  GRAEF CLAUDIA ISABELLA ,  KROGER BURKHARD ,  HABERHAUER GREGOR ,  KLOPPROGGE CORINNA ,  RUFFER UWE ,  SCHRODER HARTWIG ,  ZELDER OSKAR

IPC: C07K14/34

Abstract: The present invention relates to mutated nucleic acids and proteins of the metabolic pathway of fine chemicals, to processes for preparing genetically modified producer organisms, to processes for preparing fine chemicals by culturing genetically modified organisms, and to genetically modified organisms themselves.

公开(公告)号：ZA200404426B

公开(公告)日：2005-06-06

申请号：ZA200404426

申请日：2004-06-04

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABERHAUER GREGOR

IPC: C12N1/21 ,  C12N15/52 ,  C12N15/77 ,  C12P13/08 ,  G01N

Abstract: The invention relates to novel nucleic acid molecules, to the use thereof for constructing genetically improved microorganisms and to methods for preparing fine chemicals, in particular amino acids, with the aid of said genetically improved microorganisms.

公开(公告)号：BR0213772A

公开(公告)日：2004-10-19

申请号：BR0213772

申请日：2002-10-31

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABERHAUER GREGOR

IPC: C12N1/21 ,  C12N15/52 ,  C12P13/04 ,  C12N15/77 ,  C12N15/00

Abstract: The invention relates to novel nucleic acid molecules, to the use thereof for constructing genetically improved microorganisms and to methods for preparing fine chemicals, in particular amino acids, with the aid of said genetically improved microorganisms.

公开(公告)号：BR0213770A

公开(公告)日：2004-10-19

申请号：BR0213770

申请日：2002-10-31

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABERHAUER GREGOR

IPC: C12N1/21 ,  C12N9/12 ,  C12N15/21 ,  C12N15/31 ,  C12N15/54 ,  C12N9/00 ,  C12N15/77 ,  C12P1/04

Abstract: Isolated nucleic acid (I) that encodes any of 4 amino acid sequences (II) that have, at specific positions, an amino acid (aa) alteration are new. The specification includes (i) a table identifying the function of (II) and the nature/position of the amino acid exchange and (ii) sequences for (I) and (II). Independent claims are also included for the following: (1) Vector that contains at least one (I); (2) Host cell (C) transfected with at least one vector of (1); and (3) Preparing fine chemicals by culturing cells of (C).

公开(公告)号：AU2002368410A1

公开(公告)日：2004-06-23

申请号：AU2002368410

申请日：2002-11-29

Applicant: BASF AG

Inventor： SCHROEDER HARTWIG ,  ZELDER OSKAR ,  HABERHAUER GREGOR ,  HAEFNER STEFAN ,  KLOPPROGGE CORINNA ,  POMPEJUS MARKUS ,  KROEGER BURKHARD

IPC: C07K14/34 ,  C12N15/31 ,  C12N15/77 ,  C12P13/12 ,  C12R1/13 ,  C12R1/15

公开(公告)号：DE10239073A1

公开(公告)日：2004-03-11

申请号：DE10239073

申请日：2002-08-26

Applicant: BASF AG

Inventor： KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: A23K1/00 ,  A23K1/16 ,  C12N15/09 ,  C12P13/04 ,  C12P13/12 ,  C12P11/00 ,  C07C323/58

Abstract: Method for fermentative production of at least one sulfur-containing fine chemical (I) by fermenting a (I)-producing coryneform bacterium (A); concentrating (I) in medium or cells, then isolating it. (A) expresses at least one heterologous nucleotide sequence (II) that encodes a protein (III) with homoserine O-acetyltransferase (metA) activity. An Independent claim is also included for preparation of a feed additive (B) that contains L-methionine by culturing/fermenting an L-Met producing microorganisms; removing water from the fermentation broth and optionally also up to 100% of the biomass, then drying the treated broth to recover (B) in powdered or granular form.