公开(公告)号：EP1461119A4

公开(公告)日：2007-12-19

申请号：EP02804745

申请日：2002-12-06

Applicant: BIO RAD LABORATORIES

Inventor： RAGSDALE CHARLES W ,  MOORE JULIE K ,  HEISER WILLIAM C ,  CHIU TONY ,  KASTE KEITH

IPC: C12N15/09 ,  A61N1/30 ,  A61N1/32 ,  C12M1/00

CPC classification number: A61N1/327 ,  A61N1/0412 ,  A61N1/0476 ,  A61N1/30

Abstract: Systems, methods and algorithms for automatically performing optimization of an electroporation system. A system according to the present invention typically includes a cuvette holding assembly (80) configured to hold a plurality of electroporation cuvettes (85), wherein each cuvette includes a first and second electrode, and a shocking chamber (75) configured to hold the cuvette holding assembly, the chamber having a commutator assembly configured to provide an electrical contact to the first electrode of each of the plurality of cuvettes in turn. The system also typically includes a control system communicably coupled to the shocking chamber, wherein the control system controls the commutator (100) to automatically contact the first electrode of each cuvette in an order and to provide a potential across the cuvette electrodes when contact is made.

Abstract translation: 用于自动执行电穿孔系统优化的系统，方法和算法。 根据本发明的系统通常包括配置成保持多个电打孔比色皿（85）的比色管保持组件（80），其中每个比色池包括第一和第二电极以及配置成保持比色皿（75）的冲击室（75） 保持组件，所述腔室具有换向器组件，所述换向器组件构造成依次提供到所述多个比色杯中的每个比色杯的第一电极的电接触。 该系统通常还包括可通信地耦合到冲击室的控制系统，其中控制系统控制换向器（100）按照顺序自动地接触每个比色皿的第一电极并在接触时提供通过比色管电极的电势 。

公开(公告)号：EP1458846A4

公开(公告)日：2005-02-09

申请号：EP02791391

申请日：2002-12-06

Applicant: BIO RAD LABORATORIES

Inventor： RAGSDALE CHARLES W

IPC: C12M1/00 ,  C12M1/42 ,  C12M3/00 ,  C12N13/00 ,  C12N15/02

CPC classification number: C12M35/02

Abstract: Cuvette inserts adapted and configured to fit within an electroporation cuvette. The inserts each include a support structure that holds a porous membrane. When positioned within the cuvette, the membrane is positioned proximal the cuvette electrodes to facilitate membrane-based fusion of cells. In certain aspects, a tube extends through the support structure to allow for application of negative pressure in a convenient location away from electrode contacts and other components of the cuvette or cuvette holder.

Abstract translation: 比色皿插件适配和配置以配合在电穿孔比色皿中。 插入件包括保持多孔膜的支撑结构。 当定位在比色皿中时，膜定位在比色杯电极的近侧，以促进细胞的膜基融合。 在某些方面，管延伸穿过支撑结构，以允许在方便的位置施加负压，远离电极接触件和比色皿或比色杯保持器的其它部件。

公开(公告)号：EP2099897A4

公开(公告)日：2012-12-19

申请号：EP07864765

申请日：2007-11-26

Applicant: BIO RAD LABORATORIES

Inventor： RAGSDALE CHARLES W

IPC: C12M1/42

CPC classification number: C12N13/00 ,  C12M35/02

公开(公告)号：EP1641563A4

公开(公告)日：2012-01-18

申请号：EP04752947

申请日：2004-05-20

Applicant: BIO RAD LABORATORIES

Inventor： ARCINIEGAS GERMAN ,  CEREMONY JEFF ,  CHU DANIEL Y ,  RAGSDALE CHARLES W

IPC: B01L7/00 ,  B01L3/00 ,  B01L9/06 ,  C12M1/34 ,  C12P19/34 ,  C12Q1/68

CPC classification number: B01L7/54 ,  B01L3/50851 ,  B01L7/52 ,  B01L9/06 ,  B01L2300/044 ,  B01L2300/0829 ,  B01L2300/12 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1838 ,  B01L2300/1883 ,  B01L2400/0475 ,  B01L2400/0487 ,  B01L2400/049

Abstract: Individual temperature control in multiple reactions performed simultaneously in a spatial array such as a multi-well plate is achieved by thermoelectric modules with individual control, with each module supplying heat to or drawing heat from a single region within the array, the region containing either a single reaction vessel or a group of reaction vessels.

公开(公告)号：EP1190075A4

公开(公告)日：2003-04-16

申请号：EP00928922

申请日：2000-05-09

Applicant: BIO RAD LABORATORIES

Inventor： RAGSDALE CHARLES W ,  TAGLIAMONTE JOHN

IPC: C12M1/00 ,  C12M1/42 ,  C12N15/64 ,  C12M3/00

CPC classification number: C12M35/02

Abstract: An electroporation system and method for directing high-voltage currents to a suspension of cells contained in a cuvette. A high-voltage switch controls the coupling of a charge control to a high-voltage capacitor and, additionally, controls the coupling of the high-voltage capacitor to the cuvette. A current diverter diverts current away from the sample whenever an arc condition commences or a low sample resistance is detected across the cuvette. The current diverter includes a current diverter switch, which is triggered when a sense resistor measures a second predetermined voltage indicative of an arc-over event (or a low sample resistance condition).