公开(公告)号：KR20200128105A

公开(公告)日：2020-11-11

申请号：KR20207028036

申请日：2019-03-01

Applicant: LIFE TECHNOLOGIES CORP

Inventor： MULLAH KHAIRUZZAMAN BASHAR ,  EVANS BRIAN ,  BENSON SCOTT C ,  CHANG CHU AN ,  YAN XIONGWEI

IPC: C12Q1/6818 ,  C09B11/24 ,  C12Q1/686

Abstract: 생물학적검정법에사용되는프로브가개시된다. 프로브는올리고뉴클레오티드링커를통해소광제화합물에결합된형광염료를포함한다. 또한정량적 PCR 반응 (qPCR)에서와같은예컨대중합효소연쇄반응 (PCR)용프로브를사용하는방법및 상기프로브를포함하는키트가개시된다.

公开(公告)号：WO2016090076A3

公开(公告)日：2016-08-11

申请号：PCT/US2015063593

申请日：2015-12-03

Applicant: LIFE TECHNOLOGIES CORP

Inventor： ZHOU WENJUN ,  GEE KYLE ,  TENNICO YOLANDA ,  SLADE PETER ,  KANG HEE CHOL ,  KHAN SHAHEER ,  EVANS BRIAN ,  STRAY JAMES

IPC: C07D261/00 ,  G01N33/58

CPC classification number: C07C309/47 ,  C07C309/51 ,  C07C2603/50 ,  C07D311/84 ,  G01N33/582 ,  G01N2400/10

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

Abstract translation: 本公开内容涉及含有肼基取代基和任选地一个或多个带负电基团（例如磺酸根，磷酸根，膦酸根和/或羧酸根基团）和含有氨氧基取代基的染料化合物的染料化合物。 这些化合物可用于检测含有醛和酮基的分析物，包括例如聚糖。

公开(公告)号：WO2012125220A3

公开(公告)日：2012-11-01

申请号：PCT/US2012021264

申请日：2012-01-13

Applicant: LIFE TECHNOLOGIES CORP ,  XU TOM ,  TRINH CHRISTOPHER ,  BORNARTH CAROLE ,  EVANS BRIAN ,  RATH MOUSUMI ,  TRAN KATHY

Inventor： XU TOM ,  TRINH CHRISTOPHER ,  BORNARTH CAROLE ,  EVANS BRIAN ,  RATH MOUSUMI ,  TRAN KATHY

IPC: C12N15/10 ,  C12Q1/48 ,  C12Q1/68 ,  G01N33/53

CPC classification number: C07H21/02 ,  C12N15/1006 ,  C12Q1/6809 ,  C12Q1/6834 ,  C12Q1/6876 ,  C12Q2600/178 ,  C12Q2525/207 ,  C12Q2563/149

Abstract: Method and compositions and kits for isolation, identification, and quantification of miRNAs and other small RNAs, including but not limited to, siRNAs, mRNAs, and snRNAs are disclosed. Methods of diagnosing a disease or its progression are also disclosed.

Abstract translation: 公开了用于分离，鉴定和量化miRNA和其他小RNA（包括但不限于siRNA，mRNA和snRNA）的方法和组合物以及试剂盒。 还公开了诊断疾病或其进展的方法。

公开(公告)号：EP2663639A4

公开(公告)日：2015-06-17

申请号：EP12757380

申请日：2012-01-13

Applicant: LIFE TECHNOLOGIES CORP

Inventor： XU TOM ,  TRINH CHRISTOPHER ,  BORNARTH CAROLE ,  EVANS BRIAN ,  RATH MOUSUMI ,  TRAN KATHY

IPC: C12N15/10 ,  C07H21/02 ,  C12Q1/48 ,  C12Q1/68 ,  G01N33/53

CPC classification number: C07H21/02 ,  C12N15/1006 ,  C12Q1/6809 ,  C12Q1/6834 ,  C12Q1/6876 ,  C12Q2600/178 ,  C12Q2525/207 ,  C12Q2563/149

Abstract: Method and compositions and kits for isolation, identification, and quantification of miRNAs and other small RNAs, including but not limited to, siRNAs, mRNAs, and snRNAs are disclosed. Methods of diagnosing a disease or its progression are also disclosed.

Abstract translation: 公开了用于分离，鉴定和定量miRNA和其它小RNA（包括但不限于siRNA，mRNA和snRNA）的方法和组合物和试剂盒。 还公开了诊断疾病或其进展的方法。

公开(公告)号：SG11202106124UA

公开(公告)日：2021-07-29

申请号：SG11202106124U

申请日：2019-12-20

Applicant: LIFE TECHNOLOGIES CORP

Inventor： EVANS BRIAN ,  BENSON SCOTT ,  MULLAH KHAIRUZZAMAN BASHAR ,  YAN XIONGWEI

IPC: C09B11/24 ,  C07H21/00 ,  C12N15/65 ,  G01N33/58

Abstract: The present invention is directed to fluorescent rhodamine dyes having spectral properties suited to the creation of multiplex assay systems for use in molecular biology, cell biology and molecular genetics. The rhodamine dyes have the following structure:

公开(公告)号：SG11202101282QA

公开(公告)日：2021-03-30

申请号：SG11202101282Q

申请日：2019-08-08

Applicant: LIFE TECHNOLOGIES CORP

Inventor： YANG JONGTAE ,  WANG XIN ,  HU YI-ZHEN ,  GEE KYLE ,  CHEN AIMEI ,  BENSON SCOTT ,  EVANS BRIAN ,  RUKAVISHNIKOV ALEKSEY

IPC: C09B69/00 ,  C09B11/14 ,  C12Q1/6869 ,  G01N33/58

Abstract: Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are SiR dyes comprising at least one vinyl group attached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of SiR compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples.

公开(公告)号：CL2020002238A1

公开(公告)日：2020-12-04

申请号：CL2020002238

申请日：2020-08-31

Applicant: LIFE TECHNOLOGIES CORP

Inventor： MULLAH KHAIRUZZAMAN BASHAR ,  EVANS BRIAN ,  BENSON SCOTT C ,  CHANG CHU-AN ,  YAN XIONGWEI

IPC: C07H21/00 ,  C12Q1/6818 ,  C12Q1/686

Abstract: Se describe una sonda para su uso en ensayos biológicos. La sonda incluye un colorante fluorescente unido a un compuesto desactivador a través de un enlazador oligonucleotídico. También se describen métodos de uso de la sonda, tales como para una reacción en cadena de la polimerasa (PCR), tal como en una reacción de PCR cuantitativa (qPCR), así como kits que incluyen la sonda.

公开(公告)号：AU2023300374A1

公开(公告)日：2025-01-16

申请号：AU2023300374

申请日：2023-06-29

Applicant: LIFE TECHNOLOGIES CORP

Inventor： SHANNON MARK ,  GJERSTAD CARMEN ,  LEONG HARRISON ,  ZAHEDI MOHAMMAD MEHDI ,  EVANS BRIAN ,  MULLAH KHAIRUZZAMAN BASHAR

IPC: C12Q1/6816 ,  C09B11/24 ,  C12Q1/686

Abstract: Disclosed are compositions, kits, and methods that enable intra-channel multiplexing by enabling determination of separate detectable signals, each associated with a different assay target, within the same detection channel. The multiple detectable signals can be separately resolved and independently analyzed to enable detection and/or quantification of each respective target. Enabling multiple targets to be assayed within the same detection channel increases the plexy of multiplex assays without relying on additional dyes and concomitant issues of increased spectral overlap.

公开(公告)号：SG11202008130QA

公开(公告)日：2020-09-29

申请号：SG11202008130Q

申请日：2019-03-01

Applicant: LIFE TECHNOLOGIES CORP

Inventor： MULLAH KHAIRUZZAMAN BASHAR ,  EVANS BRIAN ,  BENSON SCOTT C ,  CHANG CHU-AN ,  YAN XIONGWEI

IPC: C07H21/00 ,  C12Q1/6818 ,  C12Q1/686

Abstract: Disclosed is a probe for use in biological assays. The probe includes a fluorescent dye bound to a quencher compound through an oligonucleotide linker. Also disclosed are methods of using the probe, such as for a polymerase chain reaction (PCR), such as in a quantitative PCR reaction (qPCR), as well as kits including the probe.

公开(公告)号：CA3124361A1

公开(公告)日：2020-06-25

申请号：CA3124361

申请日：2019-12-20

Applicant: LIFE TECHNOLOGIES CORP

Inventor： EVANS BRIAN ,  BENSON SCOTT

IPC: C09B11/24 ,  C07H21/00 ,  C12N15/65 ,  G01N33/58

Abstract: The present disclosure relates to N-protected NH-rhodamine dyes and their use in nucleic acid detection. In particular, the disclosure relates to methods of making N-protected NH-rhodamine dyes, and methods of use of N-protected NH-rhodamine dyes (e.g., human identification). Certain dyes provided herein have unique spectral properties that complement those in existing dye sets and can be used to expand the number of reporter dyes that can be included for HID applications and other biological assays. Those fluorescent compounds are useful to label synthetic oligonucleotides. Formula (I).