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公开(公告)号：KR101024975B1
公开(公告)日：2011-03-25
申请号：KR1020090031074
申请日：2009-04-10
Applicant: 건국대학교 산학협력단 , 서울대학교산학협력단
Abstract: 본 발명은 리케차 질환 진단항원의 생산방법에 관한 것으로, 더욱 상세하게는
R.
conorii 의 OmpA 또는 OmpB와 MBP(maltose-binding protein)를 포함하는 재조합 진단항원의 생산방법에 관한 것이다.
리케차, 재조합 OmpA, 재조합 OmpB-
公开(公告)号：KR1020100112678A
公开(公告)日：2010-10-20
申请号：KR1020090031074
申请日：2009-04-10
Applicant: 건국대학교 산학협력단 , 서울대학교산학협력단
Abstract: PURPOSE: A method for diagnosis antigen for rickettsiosis is provided to use as a diagnosis marker for rickettsiosi. CONSTITUTION: A method for preparing diagnosis antigen for rickettsiosi comprises: a step of isolating R. conorii OmpA gene(AE008674) or OmpB gene(AE0085659) to fragments; a step of inserting gene fragment into an expression vector having MBP gene to obtain a recombinant gene; a step of transforming the expression vector to E.coli and culturing; and a step of isolating and purifying the recombinant protein of OmpA or OmpB from E.coli. The R. conorii OmpA gene(AE008674) fragment and R. conorii OmpB gene(AE008659) is OmpA4048-5352 and OmpB2401-3807 or OmpB3679-4902.
Abstract translation: 目的：提供用于诊断抗病克氏病的方法，用作立克次体诊断标记物。 构成：用于制备立克次体诊断抗原的方法包括：将番茄红素OmpA基因（AE008674）或OmpB基因（AE0085659）分离成片段的步骤; 将基因片段插入到具有MBP基因的表达载体中以获得重组基因的步骤; 将表达载体转化到大肠杆菌并培养的步骤; 以及从大肠杆菌中分离和纯化OmpA或OmpB的重组蛋白的步骤。 R.cororii OmpA基因（AE008674）片段和R. conorii OmpB基因（AE008659）是OmpA4048-5352和OmpB2401-3807或OmpB3679-4902。
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公开(公告)号：KR100657866B1
公开(公告)日：2006-12-15
申请号：KR1020050069630
申请日：2005-07-29
Applicant: 건국대학교 산학협력단
IPC: C12Q1/68
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公开(公告)号：KR1020070014736A
公开(公告)日：2007-02-01
申请号：KR1020050069631
申请日：2005-07-29
Applicant: 건국대학교 산학협력단
IPC: C12Q1/68
CPC classification number: Y02A50/59 , C12Q1/686 , C12Q1/6837 , C12Q2537/143
Abstract: A process for differentially diagnosing rickettsial disease is provided to simultaneously diagnose spotted fever and murine typhus, and identify rickettsia strains related to spotted fever and murine typhus, so that disease associated with rickettsia is prevented and treated. A kit for differentially diagnosing rickettsial disease comprises a PCR primer for a rickettsia specific gene, wherein the rickettsia specific gene is an outer membrane protein of rickettsia, romp B; the kit further contains citric acid synthetase of rickettsia, gltA; the PCR is the multiplex-nested polymerase chain reaction; the rickettsia strain is R. conorii, R. japonica, R. felis, R. akari or R. typhi; the primer for the rickettsia specific gene is an oligonucleotide comprising the nucleotide sequence of SEQ ID NO:1 to SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:9; and the PCR product is H1 of 420bp for spotted fever or H2 of 230bp for murine typhus.
Abstract translation: 提供了一种差异性诊断立克次体疾病的方法，以同时诊断斑点斑病和鼠斑疹伤寒，并鉴定与斑疹热疹和鼠斑疹伤寒有关的立克次体菌株，从而预防和治疗与立克次体相关的疾病。 用于差异诊断立克次体疾病的试剂盒包括立克次体特异性基因的PCR引物，其中立克次体特异性基因是立克次体的外膜蛋白; 该试剂盒还含有立克次体的柠檬酸合成酶，gltA; PCR是多重巢式聚合酶链反应; 立克次体株为R. conorii，R.japonica，R.felis，R. akari或R. typhi; 立克次体特异性基因的引物是包含SEQ ID NO：1至SEQ ID NO：5，SEQ ID NO：8或SEQ ID NO：9的核苷酸序列的寡核苷酸; PCR产物的H1发现为420bp的斑点发热，H2的H2为230bp的鼠斑疹伤寒。
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Patent Agency Ranking
公开(公告)号：KR100692410B1
公开(公告)日：2007-03-09
申请号：KR1020050069631
申请日：2005-07-29
Applicant: 건국대학교 산학협력단
IPC: C12Q1/68
CPC classification number: Y02A50/59
Abstract: 본 발명은 리케차 감염 질환의 진단 방법에 관한 것으로서, 보다 상세하게는 리케차 특이 유전자를 이용한 중합효소 연쇄반응으로 리케차 균주를 선별할 수 있는 진단용 키트, 이에 사용되는 진단용 프라이머 및 이들을 이용한 리케차 질환의 진단 방법에 관한 것으로서, 이들은 홍반열 및 발진열을 용이하게 진단함과 동시에 상기 질환에 관련된 리케차 균주도 동정할 수 있게 하므로, 리케차 관련 질환을 예방하고 치료하는데 매우 유용하게 사용될 수 있다.
리케차 감염 질환, 진단용 프라이머, 홍반열, 발진열, 리케차 질환의 진단 방법-
公开(公告)号：KR101670967B1
公开(公告)日：2016-11-09
申请号：KR1020090103559
申请日：2009-10-29
Applicant: 삼성전자주식회사 , 서울대학교산학협력단
Abstract: 질병또는약물에관련된유전체마커를선택하기위한방법및 장치가개시된다. 상기유전체마커를포함하는유전체마커군이상기질병또는약물에관련된정도를나타내는평가지표를계산하고, 상기평가지표에기초하여유전체마커를선택한다.
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公开(公告)号：KR1020110046865A
公开(公告)日：2011-05-06
申请号：KR1020090103558
申请日：2009-10-29
Applicant: 서울대학교산학협력단
CPC classification number: C12Q1/6837 , C12Q1/6834 , G06F17/11 , G06F19/00
Abstract: PURPOSE: A method for determining microarray product quality is provided to digitize signal intensity by the length of a product quality management probe and to effectively determine the product quality. CONSTITUTION: A method for determining microarray product quality comprises: a step of providing a microarray containing a product quality probe nucleic acid fixed on a substrate; a step of hybridizing a sample containing signal material-labeled oligonucleotide with the probe nucleic acid; a step of measuring label signal; and a step of selecting microarray which satisfies selection criteria. The oligonucleotide contains 2-100 bases. The signal material contains fluorescent substance or radioactive substance.
Abstract translation: 目的：提供一种确定微阵列产品质量的方法，通过产品质量管理探针的长度将信号强度数字化，并有效确定产品质量。 构成：用于测定微阵列产品质量的方法包括：提供包含固定在底物上的产品质量探针核酸的微阵列的步骤; 将含有信号材料标记的寡核苷酸的样品与探针核酸杂交的步骤; 测量标签信号的步骤; 以及选择满足选择标准的微阵列的步骤。 寡核苷酸含有2-100个碱基。 信号材料含有荧光物质或放射性物质。
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公开(公告)号：KR1020110046866A
公开(公告)日：2011-05-06
申请号：KR1020090103559
申请日：2009-10-29
Applicant: 삼성전자주식회사 , 서울대학교산학협력단
CPC classification number: G06F19/18 , C12Q1/68 , C12Q2600/156
Abstract: PURPOSE: A method for selecting a gene marker relating to diseases or drugs is provided to select a gene marker group of excellent performance. CONSTITUTION: A method for selecting a gene marker comprises: a step of providing an object gene; a step of collecting a plurality of gene markers corresponding to the object gene; a step of determining standard infect of evaluation; a step of calculating the index of evaluation of a gene marker group containing two or more gene markers; and a step of selecting gene marker having optimal evaluation index. The gene marker is SNP(Single Nucleotide Polymorphism), CNV(Copy Number Variation), STS(Sequence Tagged Site), STR(Short Tandem Repeat), LTR(Long Terminal Repeat), or Indel(Insertion Deletion).
Abstract translation: 目的：提供选择与疾病或药物相关的基因标记的方法，以选择性能优异的基因标记组。 构成：选择基因标记物的方法包括：提供对象基因的步骤; 收集与所述对象基因相对应的多个基因标记物的步骤; 确定评价标准感染的一个步骤; 计算含有两个以上基因标记物的基因标记组的评价指标的步骤; 选择具有最佳评价指标的基因标记的步骤。 基因标记是SNP（单核苷酸多态性），CNV（拷贝数变异），STS（序列标记位点），STR（短串联重复），LTR（长末端重复）或Indel（插入缺失）。
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