公开(公告)号：KR1020100028839A

公开(公告)日：2010-03-15

申请号：KR1020080087767

申请日：2008-09-05

Applicant: 경남과학기술대학교 산학협력단

Inventor： 구영민 ,  김태원 ,  박갑호 ,  이철호 ,  조강진 ,  정정한 ,  이신우

IPC: C12N15/64 ,  C12N15/29 ,  C12N15/09

Abstract: PURPOSE: A transformant having high content of methionine using perilla-derived seed storage protein is provided to specifically overexpress PrLeg in potato tuber and produce crops having rich necessary amino acid content. CONSTITUTION: An expression vector contains a gene of perilla-derived seed storage protein. A promoter of a gene for tuber-specific expression is Patatin promoter derived from potato. The gene of seed storage protein is nucleic acid encoding PrLeg protein. The expression vector is CaMV35S::PrLeg/pSJ001, or Patatin::PrLeg/pSJpatatin01. A method for preparing a transformant having high content of methionine comprises: a step of preparing the expression vector; a step of transforming plant tissue with the expression vector; and a step of selecting transformant in which the expression vector is introduced.

Abstract translation: 目的：提供使用紫苏源种子储存蛋白的蛋氨酸含量高的转化体，以特异性地过量表达马铃薯块茎中的PrLeg，并产生具有丰富必需氨基酸含量的作物。 构成：表达载体含有紫苏源种子储存蛋白的基因。 用于块茎特异性表达的基因的启动子是来源于马铃薯的Patatin启动子。 种子储存蛋白的基因是编码PrLeg蛋白的核酸。 表达载体是CaMV35S :: PrLeg / pSJ001或Patatin :: PrLeg / pSJpatatin01。 制备甲硫氨酸含量高的转化体的方法包括：制备表达载体的步骤; 用表达载体转化植物组织的步骤; 以及选择其中引入表达载体的转化体的步骤。