公开(公告)号：KR102055287B1

公开(公告)日：2019-12-16

申请号：KR1020170158406

申请日：2017-11-24

Applicant: 재단법인 경남한방항노화연구원 ,  경남과학기술대학교 산학협력단 ,  주식회사 진생바이오 농업회사법인

Inventor： 구영민 ,  조계만 ,  이동열 ,  길영숙 ,  정원민 ,  이형환 ,  황정은 ,  김수철 ,  신승미

IPC: A23L19/10 ,  A23P10/40 ,  A23L33/00 ,  A23L5/10 ,  A23L29/00

公开(公告)号：KR1020170024388A

公开(公告)日：2017-03-07

申请号：KR1020150119573

申请日：2015-08-25

Applicant: 경남과학기술대학교 산학협력단

Inventor： 이신우 ,  김윤희 ,  신용욱 ,  한은희 ,  구영민 ,  조계만 ,  김만배

IPC: C12Q1/68

Abstract: 본발명은단일염기다형성(Single Nucleotide Polymorphism)을이용한하수오, 백수오및 이엽우피소의판별을마커에관한것으로, 보다구체적으로는엽록체에존재하는 TrnL, TrnF, matK 유전자단편유래의특정프라이머세트를이용하여하수오, 백수오및 이엽우피소의품종, 혼합비율및 교잡종여부등에대한정보를포함하는하수오, 백수오및 이엽우피소판별방법에관한것이다.

公开(公告)号：KR1020100028839A

公开(公告)日：2010-03-15

申请号：KR1020080087767

申请日：2008-09-05

Applicant: 경남과학기술대학교 산학협력단

Inventor： 구영민 ,  김태원 ,  박갑호 ,  이철호 ,  조강진 ,  정정한 ,  이신우

IPC: C12N15/64 ,  C12N15/29 ,  C12N15/09

Abstract: PURPOSE: A transformant having high content of methionine using perilla-derived seed storage protein is provided to specifically overexpress PrLeg in potato tuber and produce crops having rich necessary amino acid content. CONSTITUTION: An expression vector contains a gene of perilla-derived seed storage protein. A promoter of a gene for tuber-specific expression is Patatin promoter derived from potato. The gene of seed storage protein is nucleic acid encoding PrLeg protein. The expression vector is CaMV35S::PrLeg/pSJ001, or Patatin::PrLeg/pSJpatatin01. A method for preparing a transformant having high content of methionine comprises: a step of preparing the expression vector; a step of transforming plant tissue with the expression vector; and a step of selecting transformant in which the expression vector is introduced.

Abstract translation: 目的：提供使用紫苏源种子储存蛋白的蛋氨酸含量高的转化体，以特异性地过量表达马铃薯块茎中的PrLeg，并产生具有丰富必需氨基酸含量的作物。 构成：表达载体含有紫苏源种子储存蛋白的基因。 用于块茎特异性表达的基因的启动子是来源于马铃薯的Patatin启动子。 种子储存蛋白的基因是编码PrLeg蛋白的核酸。 表达载体是CaMV35S :: PrLeg / pSJ001或Patatin :: PrLeg / pSJpatatin01。 制备甲硫氨酸含量高的转化体的方法包括：制备表达载体的步骤; 用表达载体转化植物组织的步骤; 以及选择其中引入表达载体的转化体的步骤。

公开(公告)号：KR1020150019364A

公开(公告)日：2015-02-25

申请号：KR1020130096126

申请日：2013-08-13

Applicant: 경남과학기술대학교 산학협력단

Inventor： 이신우 ,  김태원 ,  구영민 ,  이민경

IPC: A01H4/00 ,  A01H1/06 ,  C12N15/82

CPC classification number: C12N15/8205 ,  A01H4/005 ,  A01H4/008 ,  C12N5/0025 ,  C12N2500/34

Abstract: The present invention relates to a method for increasing the transformation efficiency when a transformed plant with foreign genes introduced by redifferentiating potato plants in vitro or by using agrobacteria is developed. The manufacturing method of transformed potato plants of the present invention uses pieces collected from the plant grown from a novel shoot emerged from a micro tuber of a potato or the plant subcultured twice or less in the process of performing transformation, co-cultivates agrobacteria including recombination carriers and plant tissue pieces, and uses glucose instead of sucrose, which has been used previously, as a carbon source in a shoot medium, thereby increasing the redifferentiation rate of potato plants and having the effect of transforming 10% or more of the redifferentiated plants even from the potato cultivars having a very low transformation efficiency. Accordingly, if the method of the present invention is applied to the transformation of potatoes, a plant transformation system with a higher efficiency than the conventional method can be established. Furthermore, the present invention has a relatively low transformation efficiency according to some cultivars or systems, and can be usefully used in developing and commercializing genetically modified potatoes with a specific gene introduced.

Abstract translation: 本发明涉及一种在体外通过再分化马铃薯植物或通过使用农杆菌引入外源基因的转化植物时，提高转化效率的方法。 本发明的转化马铃薯植物的制造方法使用从进行转化的过程中从马铃薯微块苗或传代培养两次或更少的植物生长的植物收集的片段，共同培养包括重组的农杆菌 载体和植物组织片，并且使用先前已经使用的葡萄糖代替蔗糖作为芽培养基中的碳源，从而增加马铃薯植物的再分化速率并具有转化10％或更多的再分化植物的作用 甚至来自具有非常低的转化效率的马铃薯品种。 因此，如果将本发明的方法应用于马铃薯的转化，则可以建立具有比常规方法更高效率的植物转化系统。 此外，根据一些品种或系统，本发明具有相对低的转化效率，并且可以有用地用于开发和商业化具有引入的特定基因的转基因马铃薯。

公开(公告)号：KR1020160134282A

公开(公告)日：2016-11-23

申请号：KR1020150068094

申请日：2015-05-15

Applicant: 경남과학기술대학교 산학협력단

Inventor： 이신우 ,  김윤희 ,  신용욱 ,  조영손 ,  한은희 ,  구영민 ,  조계만 ,  김만배 ,  박춘근

IPC: C12Q1/68

Abstract: 본발명은국내에서자생하는도라지계통들로부터순계분리에의하여품종으로등록한장백도라지계통들을판별하기위하여엽록체게놈에존재하는유전자를분리하여장백도라지계통에만특이하게존재하는단일염기다형성(single nucleotide polymorphism, SNP)을이용하여서열번호 1 및 2로표시되는프라이머를사용함으로써유전자단편을 PCR로증폭하여장백도라지계통의판별이가능한마커를제공한다. 또한본 발명은 SNP를중앙에배치하여디자인한서열번호 3 및 4로표시되는프라이머를이용하여유전자단편을증폭한후 고해상도융해(high resolution melting, HRM) 곡선분석을함으로써, 장백도라지계통을판별하는방법을제공한다.

公开(公告)号：KR1020090021655A

公开(公告)日：2009-03-04

申请号：KR1020070086341

申请日：2007-08-28

Applicant: 경남과학기술대학교 산학협력단

Inventor： 구영민 ,  전현진 ,  김태원 ,  이철호 ,  배신철 ,  조강진 ,  천재안 ,  정정한 ,  이신우

IPC: A01H1/00

CPC classification number: C12N15/8271 ,  C12N9/0051 ,  C12N15/8205 ,  C12N15/8243 ,  C12Y108/05001

Abstract: Provided is a method for preparing a transformed potato which includes the increased contents of vitamin C in a tuber and a leaf, and improve resistivity to external stress so that the transformed potato grows up well. A transformed potato is transformed to a dihydroascorbate reductase gene derived from a hairy root of sesame. The increased amount of dihydroascorbate reductase gene is expressed in a leaf of the potato when compared with a wild potato under promoter control of a gene for general expression. The promoter of the gene for general expression is a CaMV35S promoter. The increased amount of dihydroascorbate reductase gene is expressed in a tuber of the potato when compared with the wild potato under promoter control of a gene for tuber specific expression. The promoter of the gene for tuber specific expression is a promoter of a patatin gene.

Abstract translation: 提供了一种制备转化马铃薯的方法，其包括在块茎和叶中维生素C的含量增加，并提高对外部胁迫的电阻率，使得转化的马铃薯生长良好。 将转化的马铃薯转化为来源于芝麻毛根的二氢抗坏血酸还原酶基因。 当与用于一般表达的基因的启动子控制下的野马铃薯相比时，在马铃薯的叶中表达增加量的二氢抗坏血酸还原酶基因。 用于一般表达的基因的启动子是CaMV35S启动子。 当与用于块茎特异性表达的基因的启动子控制下的野马铃薯相比，在马铃薯块茎中增加的二氢抗坏血酸还原酶基因的量被表达。 块茎特异性表达基因的启动子是patatin基因的启动子。