公开(公告)号：KR1020100028839A

公开(公告)日：2010-03-15

申请号：KR1020080087767

申请日：2008-09-05

Applicant: 경남과학기술대학교 산학협력단

Inventor： 구영민 ,  김태원 ,  박갑호 ,  이철호 ,  조강진 ,  정정한 ,  이신우

IPC: C12N15/64 ,  C12N15/29 ,  C12N15/09

Abstract: PURPOSE: A transformant having high content of methionine using perilla-derived seed storage protein is provided to specifically overexpress PrLeg in potato tuber and produce crops having rich necessary amino acid content. CONSTITUTION: An expression vector contains a gene of perilla-derived seed storage protein. A promoter of a gene for tuber-specific expression is Patatin promoter derived from potato. The gene of seed storage protein is nucleic acid encoding PrLeg protein. The expression vector is CaMV35S::PrLeg/pSJ001, or Patatin::PrLeg/pSJpatatin01. A method for preparing a transformant having high content of methionine comprises: a step of preparing the expression vector; a step of transforming plant tissue with the expression vector; and a step of selecting transformant in which the expression vector is introduced.

Abstract translation: 目的：提供使用紫苏源种子储存蛋白的蛋氨酸含量高的转化体，以特异性地过量表达马铃薯块茎中的PrLeg，并产生具有丰富必需氨基酸含量的作物。 构成：表达载体含有紫苏源种子储存蛋白的基因。 用于块茎特异性表达的基因的启动子是来源于马铃薯的Patatin启动子。 种子储存蛋白的基因是编码PrLeg蛋白的核酸。 表达载体是CaMV35S :: PrLeg / pSJ001或Patatin :: PrLeg / pSJpatatin01。 制备甲硫氨酸含量高的转化体的方法包括：制备表达载体的步骤; 用表达载体转化植物组织的步骤; 以及选择其中引入表达载体的转化体的步骤。

公开(公告)号：KR1020090021655A

公开(公告)日：2009-03-04

申请号：KR1020070086341

申请日：2007-08-28

Applicant: 경남과학기술대학교 산학협력단

Inventor： 구영민 ,  전현진 ,  김태원 ,  이철호 ,  배신철 ,  조강진 ,  천재안 ,  정정한 ,  이신우

IPC: A01H1/00

CPC classification number: C12N15/8271 ,  C12N9/0051 ,  C12N15/8205 ,  C12N15/8243 ,  C12Y108/05001

Abstract: Provided is a method for preparing a transformed potato which includes the increased contents of vitamin C in a tuber and a leaf, and improve resistivity to external stress so that the transformed potato grows up well. A transformed potato is transformed to a dihydroascorbate reductase gene derived from a hairy root of sesame. The increased amount of dihydroascorbate reductase gene is expressed in a leaf of the potato when compared with a wild potato under promoter control of a gene for general expression. The promoter of the gene for general expression is a CaMV35S promoter. The increased amount of dihydroascorbate reductase gene is expressed in a tuber of the potato when compared with the wild potato under promoter control of a gene for tuber specific expression. The promoter of the gene for tuber specific expression is a promoter of a patatin gene.

Abstract translation: 提供了一种制备转化马铃薯的方法，其包括在块茎和叶中维生素C的含量增加，并提高对外部胁迫的电阻率，使得转化的马铃薯生长良好。 将转化的马铃薯转化为来源于芝麻毛根的二氢抗坏血酸还原酶基因。 当与用于一般表达的基因的启动子控制下的野马铃薯相比时，在马铃薯的叶中表达增加量的二氢抗坏血酸还原酶基因。 用于一般表达的基因的启动子是CaMV35S启动子。 当与用于块茎特异性表达的基因的启动子控制下的野马铃薯相比，在马铃薯块茎中增加的二氢抗坏血酸还原酶基因的量被表达。 块茎特异性表达基因的启动子是patatin基因的启动子。