公开(公告)号：KR1020090021655A

公开(公告)日：2009-03-04

申请号：KR1020070086341

申请日：2007-08-28

Applicant: 경남과학기술대학교 산학협력단

Inventor： 구영민 ,  전현진 ,  김태원 ,  이철호 ,  배신철 ,  조강진 ,  천재안 ,  정정한 ,  이신우

IPC: A01H1/00

CPC classification number: C12N15/8271 ,  C12N9/0051 ,  C12N15/8205 ,  C12N15/8243 ,  C12Y108/05001

Abstract: Provided is a method for preparing a transformed potato which includes the increased contents of vitamin C in a tuber and a leaf, and improve resistivity to external stress so that the transformed potato grows up well. A transformed potato is transformed to a dihydroascorbate reductase gene derived from a hairy root of sesame. The increased amount of dihydroascorbate reductase gene is expressed in a leaf of the potato when compared with a wild potato under promoter control of a gene for general expression. The promoter of the gene for general expression is a CaMV35S promoter. The increased amount of dihydroascorbate reductase gene is expressed in a tuber of the potato when compared with the wild potato under promoter control of a gene for tuber specific expression. The promoter of the gene for tuber specific expression is a promoter of a patatin gene.

Abstract translation: 提供了一种制备转化马铃薯的方法，其包括在块茎和叶中维生素C的含量增加，并提高对外部胁迫的电阻率，使得转化的马铃薯生长良好。 将转化的马铃薯转化为来源于芝麻毛根的二氢抗坏血酸还原酶基因。 当与用于一般表达的基因的启动子控制下的野马铃薯相比时，在马铃薯的叶中表达增加量的二氢抗坏血酸还原酶基因。 用于一般表达的基因的启动子是CaMV35S启动子。 当与用于块茎特异性表达的基因的启动子控制下的野马铃薯相比，在马铃薯块茎中增加的二氢抗坏血酸还原酶基因的量被表达。 块茎特异性表达基因的启动子是patatin基因的启动子。