멍게 물렁증 기생충의 유전자 및 멍게 물렁증 진단 방법
    2.
    发明公开
    멍게 물렁증 기생충의 유전자 및 멍게 물렁증 진단 방법 有权
    天竺葵引起死亡的基因及其诊断方法

    公开(公告)号:KR1020070085012A

    公开(公告)日:2007-08-27

    申请号:KR1020060098722

    申请日:2006-10-11

    CPC classification number: C12Q1/6893 A01K67/0336 C12Q2600/158

    Abstract: A gene of a parasite causing death of Halocynthia roretzi and a method for diagnosing a parasite are provided to obtain a living organism that causes perishing of Halocynthia roretzi, and to diagnose a parasite by using a part of 18S rDNA fraction causing perishing of Halocynthia roretzi. A method for diagnosing a parasite includes the steps of: separating the entire nucleic acid from Halocynthia roretzi; performing PCR reaction by having the separated nucleic acid as a template and having a front primer and a reverse primer having a partial sequence of 18SrDNA fraction having a nucleotide sequence of sequence number 1 as a primer pair; and checking the size of the product of the PCR reaction by gel electrophoresis and comparing the size of the aforesaids product with the size of the product that is anticipated from the used primer pair.

    Abstract translation: 提供了引起Halocynthia roretzi死亡的寄生虫的基因和用于诊断寄生虫的方法,以获得导致Halocynthia roretzi死亡的生物体,并且通过使用引起Halocynthia roretzi灭活的18S rDNA部分的一部分来诊断寄生虫。 用于诊断寄生虫的方法包括以下步骤:将全部核酸与Halocynthia roretzi分离; 通过使分离的核酸作为模板并具有前序引物和具有序列号为1的核苷酸序列的部分序列的18SrDNA部分的反向引物作为引物对进行PCR反应; 并通过凝胶电泳检查PCR反应产物的大小,并将无菌产物的大小与从使用的引物对预期的产物的大小进行比较。

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