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公开(公告)号：KR100358755B1
公开(公告)日：2002-11-07
申请号：KR1020000010847
申请日：2000-03-04
Applicant: 대한민국(관리부서:농촌진흥청)
IPC: A01H1/00
Abstract: 본 발명은 바이러스 저항성 토마토 및 그의 제조 방법에 관한 것으로서, 구체적으로 담배 모자이크 바이러스 (Tobacco mosaic virus, TMV)의 외피 단백질 (coat protein, CP) 유전자로 형질전환시킨 바이러스 저항성 토마토 및 CP 유전자가 도입된 아그로박테리움 (
Agrobacterium )과 토마토 조직의 공존 배양, 캘러스 등의 유도, 식물체 재분화, 순화 및 포트 이식의 과정으로 구성되는 바이러스 저항성 토마토의 제조 방법에 관한 것이다.
본 발명의 바이러스 저항성 토마토는 6 세대까지 진전되어 도입시킨 유전자가 안정하게 유지되므로 모부본용 토마토로서 유용하게 사용될 수 있다.
또한, 본 발명에 의한 제조방법은 종래의 육종방법으로는 10 - 15년 이상 소요되는 품종개발 기간을 형질전환 방법을 이용하여 대폭 단축함으로써 앞으로 유용한 식물체를 개발하는 데 크게 이바지할 수 있다.-
公开(公告)号：KR1020020066583A
公开(公告)日：2002-08-21
申请号：KR1020010001761
申请日：2001-01-12
Applicant: 대한민국(관리부서:농촌진흥청)
IPC: C07K16/12
CPC classification number: C07K16/1292 , C12N5/163
Abstract: PURPOSE: Provided are a monoclonal antibody specifically recognizing phytotoxins and a process for preparation thereof by using thaxtomin separated from Streptomyces scabies. CONSTITUTION: The monoclonal antibody recognizes phytotoxins specifically and contains thaxtomin A which is separated and purified from Streptomyces scabies S33 strain, wherein the thaxtomin A is represented by the formula(1). Its manufacturing method comprises the steps of: purifying phytotoxin, thaxtomin A, from the culture of Streptomyces scabies S33 strain; conjugating the phytotoxin with bovine serum albumin(BSA) and injecting it into a mouse to form an antibody; and selecting a monoclonal antibody from hybridoma cells by ELISA.
Abstract translation: 目的：提供特异性识别植物毒素的单克隆抗体及其通过使用从耻骨链球菌分离的thaxtomin制备的方法。 构成：单克隆抗体特异性识别植物毒素，含有从痉挛链球菌S33菌株分离和纯化的thaxtomin A，其中thaxtomin A由式（1）表示。 其制造方法包括以下步骤：从耻骨链霉菌S33菌株的培养物中纯化植物毒素，thaxtomin A; 将植物毒素与牛血清白蛋白（BSA）共轭并将其注入小鼠中以形成抗体; 并通过ELISA从杂交瘤细胞中选择单克隆抗体。
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公开(公告)号：KR100458002B1
公开(公告)日：2004-11-18
申请号：KR1020010001761
申请日：2001-01-12
Applicant: 대한민국(관리부서:농촌진흥청)
IPC: C07K16/12
Abstract: PURPOSE: Provided are a monoclonal antibody specifically recognizing phytotoxins and a process for preparation thereof by using thaxtomin separated from Streptomyces scabies. CONSTITUTION: The monoclonal antibody recognizes phytotoxins specifically and contains thaxtomin A which is separated and purified from Streptomyces scabies S33 strain, wherein the thaxtomin A is represented by the formula(1). Its manufacturing method comprises the steps of: purifying phytotoxin, thaxtomin A, from the culture of Streptomyces scabies S33 strain; conjugating the phytotoxin with bovine serum albumin(BSA) and injecting it into a mouse to form an antibody; and selecting a monoclonal antibody from hybridoma cells by ELISA.
Abstract translation: 目的：提供一种特异性识别植物毒素的单克隆抗体及其使用从链霉菌（Streptomyces scabies）中分离的沙氏菌素（thaxtomin）的制备方法。 组成：单克隆抗体特异性识别植物毒素并含有从链霉菌sc疮S33菌株中分离和纯化的沙氏菌素A，其中沙替霉素A由式（1）表示。 其制造方法包括以下步骤：从链霉菌sc疮S33菌株的培养物中纯化植物毒素thaxtomin A; 将植物毒素与牛血清白蛋白（BSA）缀合并将其注射到小鼠中以形成抗体; 并通过ELISA从杂交瘤细胞中选择单克隆抗体。
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公开(公告)号：KR1020010086888A
公开(公告)日：2001-09-15
申请号：KR1020000010847
申请日：2000-03-04
Applicant: 대한민국(관리부서:농촌진흥청)
IPC: A01H1/00
CPC classification number: C12N15/8283 , C07K14/005 , C12N15/8205 , C12N15/8209
Abstract: PURPOSE: The virus resistant tomato and its producing method are provided, which contains a virus resistant gene for more than 5 generations, so that it can be used as seed tomato. CONSTITUTION: The virus tolerant tomato, T16-11-5-3 or T44-2-6-14-28 is produced by transforming a normal tomato gene with the surface protein gene of tobacco mosaic virus (TMV). The method for producing virus resistant tomato comprises the steps of: simultaneously incubating Agrobacterium sp. containing a foreign gene such as tobacco mosaic virus (TMV) gene and tomato tissues, for 4 to 6 minutes to induce calluses and shoots; and re-differentiating the calluses and shoots and attenuating them for 6 to 8 days; and transplanting them into ports.
Abstract translation: 目的：提供病毒抗性番茄及其生产方法，其含有超过5代的病毒抗性基因，因此可用作种子番茄。 构成：通过用烟草花叶病毒（TMV）的表面蛋白质基因转化正常的番茄基因来产生病毒耐性番茄T16-11-5-3或T44-2-6-14-28。 产生耐病性番茄的方法包括以下步骤：同时培养农杆菌 含有外来基因如烟草花叶病毒（TMV）基因和番茄组织4至6分钟以诱导愈伤组织和芽; 并重新分化愈伤组织和芽，并将其减毒6至8天; 并将其移植到端口中。
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