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公开(公告)号：KR1020070121468A
公开(公告)日：2007-12-27
申请号：KR1020060056569
申请日：2006-06-22
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12Q1/68
CPC classification number: C12N15/11 , C12Q1/686 , G01N33/569
Abstract: A differential diagnostic method of duck hepatitis virus(DHV) type 1 is provided to improve rapidness and accuracy of diagnosis, and diagnose common genes and different genes of duck hepatitis virus type 1 in the liver tissue separated from young ducks infected by the duck hepatitis virus type 1, and viruses causing other RNA viral diseases derived from fowls. Duck hepatitis virus type 1 gene has the nucleotide sequence consisting of 2C gene, capsid gene and 5'-UTR(untranslated region) gene, wherein the 2C gene has the nucleotide sequence selected from SEQ ID NOs:1, 4, 7, 10, 13, 16, 19, 22 and 25; the capsid gene has the nucleotide sequence selected from SEQ ID NOs:2, 5, 8, 11, 14, 17, 20, 23 and 26; and the 5'-UTR gene has the nucleotide sequence selected from SEQ ID NOs:3, 6, 9, 12, 15, 18, 21, 24 and 27. Duck hepatitis virus-specific primers have the nucleotide sequences of SEQ ID NOs:28 to 33 and specifically recognize the 2C gene, capsid gene and 5'-UTR gene, respectively. A differential diagnostic method of duck hepatitis virus(DHV) type 1 comprises the steps of: (1) homogenizing the liver tissue separated from young ducks infected by the duck hepatitis virus type 1 to float in 10% PBS(phosphate buffered saline); (2) obtaining the supernatant and separating RNA from the supernatant by RNA extraction method; (3) synthesizing cDNA(complementary DNA) from the separated RNA through reverse transcription method by using reverse transcriptase; (4) preparing primers specific to 2C gene, capsid gene and 5'-UTR gene from cDNA, and amplifying the genes through PCR(polymerase chain reaction); and (5) subjecting the PCR products to electrophoresis using 1.5% agarose gel.
Abstract translation: 提供1型鸭肝炎病毒（DHV）的鉴别诊断方法，以提高诊断的快速性和准确性，并在鸭肝炎病毒感染的幼鸭分离的肝组织中诊断1型鸭肝炎病毒的常见基因和不同基因 类型1，以及导致其他来源于家禽的RNA病毒病毒的病毒。 鸭肝炎病毒1型基因具有由2C基因，衣壳基因和5'-UTR（非翻译区）基因组成的核苷酸序列，其中2C基因具有选自SEQ ID NO：1,4,7,10， 13，16，19，22和25; 所述衣壳基因具有选自SEQ ID NO：2,5,8,11,14,17,20,23和26的核苷酸序列; 并且5'-UTR基因具有选自SEQ ID NO：3,6,9,12,15,18,21,24和27的核苷酸序列。鸭肝炎病毒特异性引物具有SEQ ID NO： 28〜33，分别特异性地识别2C基因，衣壳基因和5'-UTR基因。 鸭肝炎病毒（DHV）1型的鉴别诊断方法包括以下步骤：（1）将由1型鸭肝炎病毒感染的幼鸭分离的肝组织匀浆在10％PBS（磷酸盐缓冲盐水）中漂浮; （2）通过RNA提取方法获得上清液和上清液中的RNA; （3）使用逆转录酶通过逆转录法从分离的RNA合成cDNA（互补DNA）; （4）从cDNA制备特异于2C基因，衣壳基因和5'-UTR基因的引物，并通过PCR（聚合酶链式反应）扩增基因; 和（5）使用1.5％琼脂糖凝胶对PCR产物进行电泳。
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公开(公告)号：KR100664995B1
公开(公告)日：2007-01-04
申请号：KR1020060002630
申请日：2006-01-10
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: A method for diagnosing duck hepatitis virus type 1 is provided to reduce the diagnosis time, improve convenience of diagnosis, and enhance diagnosis accuracy by performing PCR using a primer set specific to duck hepatitis virus type 1. The method for diagnosing duck hepatitis virus type 1 comprises the steps of: (1) pulverizing the liver tissue infected by duck hepatitis virus and suspending the pulverized liver tissue in PBS(phosphate buffer saline); (2) extracting RNA from the supernatant by using LS reagent; (3) synthesizing cDNA from the extracted RNA by using reverse transcriptase(MMLV); (4) performing PCR using synthesized cDNA as a template using 3D gene-specific primers having the nucleotide sequences of SEQ ID NOs:6 and 7; and (5) subjecting the PCR product to electrophoresis by using 1.5% agarose gel, wherein the 3D gene is derived from duck hepatitis virus type 1 and has the nucleotide sequence selected from SEQ ID NO:1 to SEQ ID NO:5.
Abstract translation: 提供一种用于诊断1型鸭肝炎病毒的方法，通过使用特异于1型鸭肝炎病毒的引物组进行PCR，减少诊断时间，提高诊断方便性，提高诊断准确度。1型鸭肝炎病毒诊断方法 包括以下步骤：（1）粉碎鸭肝炎病毒感染的肝组织，并将粉碎的肝组织悬浮于PBS（磷酸盐缓冲盐水）中; （2）使用LS试剂从上清液中提取RNA; （3）使用逆转录酶（MMLV）从提取的RNA合成cDNA; （4）使用具有SEQ ID NO：6和7的核苷酸序列的3D基因特异性引物，使用合成的cDNA作为模板进行PCR; 和（5）使用1.5％琼脂糖凝胶对PCR产物进行电泳，其中3D基因来源于1型鸭型肝炎病毒，并具有选自SEQ ID NO：1至SEQ ID NO：5的核苷酸序列。
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公开(公告)号：KR101557191B1
公开(公告)日：2015-10-02
申请号：KR1020130075529
申请日：2013-06-28
Applicant: 조지아 스테이트 유니버시티 리서치 파운데이션, 인코포레이티드 , 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C07K14/11 , C12N7/01 , A61K39/145 , A61P31/16
Abstract: 본발명은넓은범위의방어능력을갖는인플루엔자단독백신또는이를이용한인플루엔자보강백신에관한것이다. 본발명의인플루엔자단독백신또는인플루엔자보강백신은다양한혈청형의인플루엔자바이러스감염에대하여 M2e단백질에대한면역력을증가시킴으로써전국적유행병과같이예측하지못한바이러스의확산에효과적으로대처할수 있는광범위한방어능력을가진다.
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4.发明授权신속 면역크로마토그라피법에 의한 Ｈ9형 조류인플루엔자 바이러스 진단 키트 및 이를 이용하여 Ｈ9형 조류인플루엔자를 진단하는 방법 有权使用快速免疫层析技术的H9型禽流感病毒诊断试剂盒及使用相同方法诊断H9型禽流感
公开(公告)号：KR101211593B1
公开(公告)日：2013-01-07
申请号：KR1020100087163
申请日：2010-09-06
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 바이오노트
IPC: G01N33/569 , G01N33/538 , G01N33/553 , C07K16/08
Abstract: 본발명은헤마글루티닌항원이 H9형조류인플루엔자에특이적인항체를이용한신속면역크로마토그라피법에의해닭 총배설강(분변) 스왑시료에서 H9형조류인플루엔자감염여부를신속하고정확하게진단할수 있는진단키트및 이를이용하여 H9형조류인플루엔자를진단하는방법에관한것이다.
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公开(公告)号：KR100858169B1
公开(公告)日：2008-09-17
申请号：KR1020060056569
申请日：2006-06-22
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12Q1/68
Abstract: 본 발명은 오리 간염바이러스 1형(duck hepatitis virus type 1) 유전자의 감별진단방법(differential diagnostic method)에 관한 것으로, 더욱 상세하게는 2C 유전자, 캡시드(capsid) 유전자 및 5'-UTR(untranslated region) 유전자를 표적(target)하는 특이 프라이머를 사용하여 RT-PCR 방법으로 오리 간염바이러스 1형과 유전자형이 다른 오리 간염바이러스 유전자를 감별 진단하는 방법에 관한 것이다. 또한 본 발명에 따른 감별진단방법은 조류 유래의 다른 병원성 RNA 바이러스들과도 신속하고 정확하게 감별적으로 진단하는데 유용하다.
오리 간염바이러스 1형 및 아형, PCR, 5′-UTR 유전자, 캡시트 유전자, 3D 유전자-
Patent Agency Ranking
公开(公告)号：KR1020090039230A
公开(公告)日：2009-04-22
申请号：KR1020070104736
申请日：2007-10-17
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N7/00
Abstract: A new vaccine to duck hepatitis virus is provided to have excellence in resistance against epidemic duck hepatitis and lessen damage caused by the duck hepatitis. A method for producing vaccine(deposition No. KCCM10883P) of duck hepatitis virus having sequence of sequence No. 1 [SEQ ID NO:1] comprises: a step of diluting recently epidemic duck hepatitis virus in phosphate buffer; a step of inoculating 7-9 days old chicken embryo; a step of culturing the inoculated chicken embryo at 37°C for three days; and a step of collecting the cultured chicken embryo and performing subculture sequencially to adapt the chicken embryo. A process for manufacturing a new vaccine is performed with: the step of attenuating the duck hepatitis virus; a step of testing restoration of attenuated virus; a step of searching defense ability of vaccine to selected duck hepatitis virus; a step of searching defense ability in each pathway; a step of searching minimum amount of the vaccine; and a step of safety and amount of the vaccine.
Abstract translation: 提供了一种新型的鸭肝炎病毒疫苗，具有卓越的抗流行性鸭肝炎防治能力，减轻了鸭肝炎所造成的伤害。 具有序列号1 [SEQ ID NO：1]序列的鸭肝炎病毒疫苗（沉积编号KCCM10883P）的制备方法包括：在磷酸盐缓冲液中稀释近期流行的鸭肝炎病毒的步骤; 接种7-9天龄鸡胚的步骤; 将接种的鸡胚在37℃培养3天的步骤; 并收集培养的鸡胚并顺序进行亚文化以适应鸡胚的步骤。 制备新疫苗的方法是：减毒鸭肝炎病毒的步骤; 测试减毒病毒恢复的一个步骤; 检测疫苗对选择的鸭肝炎病毒的防御能力的一个步骤; 在每个途径中寻找防御能力的一个步骤; 搜索最少量的疫苗的步骤; 和疫苗的安全和数量的步骤。
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公开(公告)号：KR100834026B1
公开(公告)日：2008-06-02
申请号：KR1020070001888
申请日：2007-01-08
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K39/0208 , C07K14/195
Abstract: A riemerella vaccine for preventing riemerella infection is provided to defend the infection of field pathogenic Riemerella anatipestifer which occurs frequently in a country and prevent ducks from being perished by the riemerella infection, thereby minimizing economical loss of a duck industry. A riemerella vaccine for preventing riemerella infection comprises an inactivated Riemerella anatipestifer and an immuno-enhancing agent, wherein the inactivated Riemerella anatipestifer is a strain selected from the group consisting of serovar type I, serovar type IV, serovar type VII, serovar type XVI and a mixture strain thereof. A method for preparing the riemerella vaccine comprises the steps of: (a) culturing Riemerella anatipestifer isolated from a suck; (b) adding an inactivating agent such as 0.3% formalized physiological saline solution(FPSS) to a culture solution obtained from the step(a) to inactivate the Riemerella anatipestifer; and (c) adding an immuno-enhancing agent to the inactivated Riemerella anatipestifer culture solution to prepare a mixture vaccine. Further, the immuno-enhancing agent is ISA70.
Abstract translation: 提供了一种用于预防痢疾杆菌感染的雷米他菌疫苗，用于捍卫一个国家频繁发生的野外致病性雷米他氏菌感染，并防止鸭子被莱梅里埃菌感染消灭，从而最大限度地减少了鸭业的经济损失。 用于预防雷米他里亚感染的莱米诺菌素疫苗包括灭活的雷米他氏菌和免疫增强剂，其中所述灭活的雷米他氏菌是选自I型血清型，IV型血清型，VII型血清型，血清型XVI和 其混合应变。 一种制备莱米酵母疫苗的方法包括以下步骤：（a）培养从吸吮物分离的烟草雷蒙酵母; （b）将灭活剂如0.3％正规化生理盐水溶液（FPSS）加入到从步骤（a）获得的培养溶液中以灭活烟草雷蒙酵母; 和（c）向灭活的雷神雷公藤培养液中加入免疫增强剂以制备混合疫苗。 此外，免疫增强剂是ISA70。
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公开(公告)号：KR1020140078523A
公开(公告)日：2014-06-25
申请号：KR1020130075529
申请日：2013-06-28
Applicant: 조지아 스테이트 유니버시티 리서치 파운데이션, 인코포레이티드 , 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C07K14/11 , C12N7/01 , A61K39/145 , A61P31/16
Abstract: The present invention relates to an influenza monovalent vaccine having a broad defending activity, or an influenza supplementary vaccine using the same. The influenza monovalent vaccine or influenza supplementary vaccine of the present invention increases the immunity against an M2e protein for influenza virus infection of various serotypes, thereby having a broad defense ability effectively responding to the unforeseen spread of virus like national epidemic.
Abstract translation: 本发明涉及具有广泛防御活性的流感单价疫苗，或使用其的流感病毒补充疫苗。 本发明的流感性单价疫苗或流感病毒补充疫苗提高了针对各种血清型流感病毒感染的M2e蛋白的免疫力，从而具有有效应对国家流行病毒等不可预见的病毒传播的广泛防御能力。
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公开(公告)号：KR100959259B1
公开(公告)日：2010-05-26
申请号：KR1020070104736
申请日：2007-10-17
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N7/00
Abstract: 본 발명은 새로운 오리 간염 바이러스 백신주에 관한 것으로서, 보다 상세하게는 최근 국내에서 유행하는 오리 간염바이러스를 인산완충액에 희석하여 7-9일령 계태아에 접종하여 37℃에서 3일간 배양한 뒤 계태아를 채취하여 다시 인산완충액으로 유제하여 연속으로 계태아에 계대하여 순응시켜 제조한 오리 간염 바이러스 백신주에 관한 것이다. 본 발명에 의한 신규 오리 간염바이러스 백신은 1일령 어린 오리에서 접종할 경우 병원성이 관찰되지 않고 최근 국내에서 유행하는 오리 간염에 대해 100%의 방어능을 발휘할 수 있었다.
오리 간염바이러스 1형, 최근 국내유행 오리 간염바이러스, 백신주, 병원성, 방어능-
10.发明公开신속 면역크로마토그라피법에 의한 Ｈ9형 조류인플루엔자 바이러스 진단 키트 및 이를 이용하여 Ｈ9형 조류인플루엔자를 진단하는 방법 有权使用快速免疫印迹的H9型AVIAN流感病毒的诊断试剂盒和使用该方法诊断H9型AVIAN流感的方法
公开(公告)号：KR1020120024313A
公开(公告)日：2012-03-14
申请号：KR1020100087163
申请日：2010-09-06
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 바이오노트
IPC: G01N33/569 , G01N33/538 , G01N33/553 , C07K16/08
Abstract: PURPOSE: An H9 type avian influenza virus diagnosis kit by quick immunochromatography is provided to ensure quick and simple diagnosis without special equipment. CONSTITUTION: A kit for diagnosing H9 type avian influenza virus contains monoclonal antibodies. The monoclonal antibody is prepared from hybridoma cells of deposit number 11753BP, KCTC 11754BP, and KCTC 11755BP. A H9 type avian influenza diagnosis strip contains the monoclonal antibody. The diagnosis strip comprises a sample pad(1), a gold pad(2), nitrocellulose membrane(3), and moisture absorption pad(4). The gold pad contains a gold conjugate in which gold particles are conjugated with a monoclonal antibody produced from hybridoma cells of deposit number KCTC 11755BP.
Abstract translation: 目的：提供快速免疫色谱法的H9型禽流感病毒诊断试剂盒，无需特殊设备即可快速，简便地进行诊断。 构成：用于诊断H9型禽流感病毒的工具包含单克隆抗体。 单克隆抗体由编号11753BP，KCTC 11754BP和KCTC 11755BP的杂交瘤细胞制备。 H9型禽流感诊断条含有单克隆抗体。 诊断条包括样品垫（1），金垫（2），硝酸纤维素膜（3）和吸湿垫（4）。 金垫包含金结合物，其中金颗粒与由保藏号为KCTC 11755BP的杂交瘤细胞产生的单克隆抗体缀合。
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