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公开(公告)号：KR101503511B1
公开(公告)日：2015-03-24
申请号：KR1020130078000
申请日：2013-07-03
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 흰반점 증후군 바이러스(white spot syndrom virus; WSSV) 진단용 프라이머 세트, 올리고뉴클레오티드, 및 이를 포함하는 진단용 조성물, 진단용 키트 및 진단을 위한 정보 제공 방법에 관한 것이다. 본 발명에 따른 WSSV 외피막 단백질을 발현하는 유전자 VP19 또는 VP28과 결합하는 프라이머 세트 및 비특이반응 억제용 올리고뉴클레오티드를 이용하여 단일 튜브 이중 중합효소연쇄반응을 수행할 경우, WSSV에 대한 민감도를 개선시켜 1 카피수까지 검출가능하고, 진단 소요시간이 단축되며, 외부환경에 의한 오염을 차단하고, WSSV 검출률을 유의하게 증가시켜 WSSV를 특이적으로 검출할 수 있어, WSSV에 감염된 개체뿐만아니라, WSSV 매개체 및 보유 생물에서도 WSSV 검출이 가능하여 수생환경내 흰반점병의 감시, 전염병 조기 발견 및 통제를 위한 WSSV 유전자 진단에 유용하게 사용될 수 있다.
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公开(公告)号：KR1020150004677A
公开(公告)日：2015-01-13
申请号：KR1020130078000
申请日：2013-07-03
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: C12N15/11 , C12Q1/686 , C12Q2547/101
Abstract: 본 발명은 흰반점 증후군 바이러스(white spot syndrom virus; WSSV) 진단용 프라이머 세트, 올리고뉴클레오티드, 및 이를 포함하는 진단용 조성물, 진단용 키트 및 진단을 위한 정보 제공 방법에 관한 것이다. 본 발명에 따른 WSSV 외피막 단백질을 발현하는 유전자 VP19 또는 VP28과 결합하는 프라이머 세트 및 비특이반응 억제용 올리고뉴클레오티드를 이용하여 단일 튜브 이중 중합효소연쇄반응을 수행할 경우, WSSV에 대한 민감도를 개선시켜 1 카피수까지 검출가능하고, 진단 소요시간이 단축되며, 외부환경에 의한 오염을 차단하고, WSSV 검출률을 유의하게 증가시켜 WSSV를 특이적으로 검출할 수 있어, WSSV에 감염된 개체뿐만아니라, WSSV 매개체 및 보유 생물에서도 WSSV 검출이 가능하여 수생환경내 흰반점병의 감시, 전염병 조기 발견 및 통제를 위한 WSSV 유전자 진단에 유용하게 사용될 수 있다.
Abstract translation: 本发明涉及用于诊断白斑综合征病毒（WSSV）的引物组，寡核苷酸，诊断组合物和包含该引物组的诊断试剂盒，以及用于提供诊断信息的方法。 如果使用与表达WSSV包膜蛋白的VP19或VP28基因组合的引物组和用于抑制非特异性反应的寡核苷酸进行单管嵌套PCR：增强了对WSSV的敏感性，从而能够检测1拷贝 数; 诊断时间可以缩短; 可以防止外部环境造成的污染; WSSV检测率可以显着增加，可以特异性检测WSSV，从而能够不仅从WSSV感染的实体中检测到WSSV，而且可以从WSSV介质和WSSV所具有的生物体检测WSSV，并且可用于诊断WSSV基因以观察白色 水生环境中的斑点综合征，以及早期发现和控制传染病。
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公开(公告)号：KR1020110044427A
公开(公告)日：2011-04-29
申请号：KR1020090101085
申请日：2009-10-23
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: Y02A50/451 , C12Q1/6888 , C12N15/11 , C12Q1/6844
Abstract: PURPOSE: A method for detecting Salmonella gallinarum or probiotics for Salmonella gallinarum vaccine is provided to quickly detect Salmonella gallinarum. CONSTITUTION: A method for detecting Salmonella gallinarum or probitocs Salmonella gallinarum comprises: a step of preparing DNA from a biological sample; a step of performing PCR using the DNA as a temperate and primer; and a step of analyzing DNA fragment through from PCR product.
Abstract translation: 目的：提供一种检测沙门氏菌沙门氏菌或益生菌沙门氏菌疫苗的方法，以快速检测沙门氏菌。 构成：检测沙门氏菌或前列腺素沙门氏菌的方法包括：从生物样品制备DNA的步骤; 使用DNA作为温带和引物进行PCR的步骤; 以及通过PCR产物分析DNA片段的步骤。
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公开(公告)号：KR1020070121468A
公开(公告)日：2007-12-27
申请号：KR1020060056569
申请日：2006-06-22
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12Q1/68
CPC classification number: C12N15/11 , C12Q1/686 , G01N33/569
Abstract: A differential diagnostic method of duck hepatitis virus(DHV) type 1 is provided to improve rapidness and accuracy of diagnosis, and diagnose common genes and different genes of duck hepatitis virus type 1 in the liver tissue separated from young ducks infected by the duck hepatitis virus type 1, and viruses causing other RNA viral diseases derived from fowls. Duck hepatitis virus type 1 gene has the nucleotide sequence consisting of 2C gene, capsid gene and 5'-UTR(untranslated region) gene, wherein the 2C gene has the nucleotide sequence selected from SEQ ID NOs:1, 4, 7, 10, 13, 16, 19, 22 and 25; the capsid gene has the nucleotide sequence selected from SEQ ID NOs:2, 5, 8, 11, 14, 17, 20, 23 and 26; and the 5'-UTR gene has the nucleotide sequence selected from SEQ ID NOs:3, 6, 9, 12, 15, 18, 21, 24 and 27. Duck hepatitis virus-specific primers have the nucleotide sequences of SEQ ID NOs:28 to 33 and specifically recognize the 2C gene, capsid gene and 5'-UTR gene, respectively. A differential diagnostic method of duck hepatitis virus(DHV) type 1 comprises the steps of: (1) homogenizing the liver tissue separated from young ducks infected by the duck hepatitis virus type 1 to float in 10% PBS(phosphate buffered saline); (2) obtaining the supernatant and separating RNA from the supernatant by RNA extraction method; (3) synthesizing cDNA(complementary DNA) from the separated RNA through reverse transcription method by using reverse transcriptase; (4) preparing primers specific to 2C gene, capsid gene and 5'-UTR gene from cDNA, and amplifying the genes through PCR(polymerase chain reaction); and (5) subjecting the PCR products to electrophoresis using 1.5% agarose gel.
Abstract translation: 提供1型鸭肝炎病毒（DHV）的鉴别诊断方法，以提高诊断的快速性和准确性，并在鸭肝炎病毒感染的幼鸭分离的肝组织中诊断1型鸭肝炎病毒的常见基因和不同基因 类型1，以及导致其他来源于家禽的RNA病毒病毒的病毒。 鸭肝炎病毒1型基因具有由2C基因，衣壳基因和5'-UTR（非翻译区）基因组成的核苷酸序列，其中2C基因具有选自SEQ ID NO：1,4,7,10， 13，16，19，22和25; 所述衣壳基因具有选自SEQ ID NO：2,5,8,11,14,17,20,23和26的核苷酸序列; 并且5'-UTR基因具有选自SEQ ID NO：3,6,9,12,15,18,21,24和27的核苷酸序列。鸭肝炎病毒特异性引物具有SEQ ID NO： 28〜33，分别特异性地识别2C基因，衣壳基因和5'-UTR基因。 鸭肝炎病毒（DHV）1型的鉴别诊断方法包括以下步骤：（1）将由1型鸭肝炎病毒感染的幼鸭分离的肝组织匀浆在10％PBS（磷酸盐缓冲盐水）中漂浮; （2）通过RNA提取方法获得上清液和上清液中的RNA; （3）使用逆转录酶通过逆转录法从分离的RNA合成cDNA（互补DNA）; （4）从cDNA制备特异于2C基因，衣壳基因和5'-UTR基因的引物，并通过PCR（聚合酶链式反应）扩增基因; 和（5）使用1.5％琼脂糖凝胶对PCR产物进行电泳。
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Patent Agency Ranking
公开(公告)号：KR100664995B1
公开(公告)日：2007-01-04
申请号：KR1020060002630
申请日：2006-01-10
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: A method for diagnosing duck hepatitis virus type 1 is provided to reduce the diagnosis time, improve convenience of diagnosis, and enhance diagnosis accuracy by performing PCR using a primer set specific to duck hepatitis virus type 1. The method for diagnosing duck hepatitis virus type 1 comprises the steps of: (1) pulverizing the liver tissue infected by duck hepatitis virus and suspending the pulverized liver tissue in PBS(phosphate buffer saline); (2) extracting RNA from the supernatant by using LS reagent; (3) synthesizing cDNA from the extracted RNA by using reverse transcriptase(MMLV); (4) performing PCR using synthesized cDNA as a template using 3D gene-specific primers having the nucleotide sequences of SEQ ID NOs:6 and 7; and (5) subjecting the PCR product to electrophoresis by using 1.5% agarose gel, wherein the 3D gene is derived from duck hepatitis virus type 1 and has the nucleotide sequence selected from SEQ ID NO:1 to SEQ ID NO:5.
Abstract translation: 提供一种用于诊断1型鸭肝炎病毒的方法，通过使用特异于1型鸭肝炎病毒的引物组进行PCR，减少诊断时间，提高诊断方便性，提高诊断准确度。1型鸭肝炎病毒诊断方法 包括以下步骤：（1）粉碎鸭肝炎病毒感染的肝组织，并将粉碎的肝组织悬浮于PBS（磷酸盐缓冲盐水）中; （2）使用LS试剂从上清液中提取RNA; （3）使用逆转录酶（MMLV）从提取的RNA合成cDNA; （4）使用具有SEQ ID NO：6和7的核苷酸序列的3D基因特异性引物，使用合成的cDNA作为模板进行PCR; 和（5）使用1.5％琼脂糖凝胶对PCR产物进行电泳，其中3D基因来源于1型鸭型肝炎病毒，并具有选自SEQ ID NO：1至SEQ ID NO：5的核苷酸序列。
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公开(公告)号：KR101566376B1
公开(公告)日：2015-11-09
申请号：KR1020120156224
申请日：2012-12-28
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본발명은닭에서전염성활막염과호흡기증상을유발하는주요병원성세균인마이코플라즈마시노비에() 국내분리주에관한것으로, 보다구체적으로는국내사육닭에서분리되고, 높은전염성및 면역원성을갖는분리주중MS-09-AR 백신주(기탁번호 : KCTC12342BP)에관한것이다.
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公开(公告)号：KR1020090039230A
公开(公告)日：2009-04-22
申请号：KR1020070104736
申请日：2007-10-17
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N7/00
Abstract: A new vaccine to duck hepatitis virus is provided to have excellence in resistance against epidemic duck hepatitis and lessen damage caused by the duck hepatitis. A method for producing vaccine(deposition No. KCCM10883P) of duck hepatitis virus having sequence of sequence No. 1 [SEQ ID NO:1] comprises: a step of diluting recently epidemic duck hepatitis virus in phosphate buffer; a step of inoculating 7-9 days old chicken embryo; a step of culturing the inoculated chicken embryo at 37°C for three days; and a step of collecting the cultured chicken embryo and performing subculture sequencially to adapt the chicken embryo. A process for manufacturing a new vaccine is performed with: the step of attenuating the duck hepatitis virus; a step of testing restoration of attenuated virus; a step of searching defense ability of vaccine to selected duck hepatitis virus; a step of searching defense ability in each pathway; a step of searching minimum amount of the vaccine; and a step of safety and amount of the vaccine.
Abstract translation: 提供了一种新型的鸭肝炎病毒疫苗，具有卓越的抗流行性鸭肝炎防治能力，减轻了鸭肝炎所造成的伤害。 具有序列号1 [SEQ ID NO：1]序列的鸭肝炎病毒疫苗（沉积编号KCCM10883P）的制备方法包括：在磷酸盐缓冲液中稀释近期流行的鸭肝炎病毒的步骤; 接种7-9天龄鸡胚的步骤; 将接种的鸡胚在37℃培养3天的步骤; 并收集培养的鸡胚并顺序进行亚文化以适应鸡胚的步骤。 制备新疫苗的方法是：减毒鸭肝炎病毒的步骤; 测试减毒病毒恢复的一个步骤; 检测疫苗对选择的鸭肝炎病毒的防御能力的一个步骤; 在每个途径中寻找防御能力的一个步骤; 搜索最少量的疫苗的步骤; 和疫苗的安全和数量的步骤。
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公开(公告)号：KR100834026B1
公开(公告)日：2008-06-02
申请号：KR1020070001888
申请日：2007-01-08
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K39/0208 , C07K14/195
Abstract: A riemerella vaccine for preventing riemerella infection is provided to defend the infection of field pathogenic Riemerella anatipestifer which occurs frequently in a country and prevent ducks from being perished by the riemerella infection, thereby minimizing economical loss of a duck industry. A riemerella vaccine for preventing riemerella infection comprises an inactivated Riemerella anatipestifer and an immuno-enhancing agent, wherein the inactivated Riemerella anatipestifer is a strain selected from the group consisting of serovar type I, serovar type IV, serovar type VII, serovar type XVI and a mixture strain thereof. A method for preparing the riemerella vaccine comprises the steps of: (a) culturing Riemerella anatipestifer isolated from a suck; (b) adding an inactivating agent such as 0.3% formalized physiological saline solution(FPSS) to a culture solution obtained from the step(a) to inactivate the Riemerella anatipestifer; and (c) adding an immuno-enhancing agent to the inactivated Riemerella anatipestifer culture solution to prepare a mixture vaccine. Further, the immuno-enhancing agent is ISA70.
Abstract translation: 提供了一种用于预防痢疾杆菌感染的雷米他菌疫苗，用于捍卫一个国家频繁发生的野外致病性雷米他氏菌感染，并防止鸭子被莱梅里埃菌感染消灭，从而最大限度地减少了鸭业的经济损失。 用于预防雷米他里亚感染的莱米诺菌素疫苗包括灭活的雷米他氏菌和免疫增强剂，其中所述灭活的雷米他氏菌是选自I型血清型，IV型血清型，VII型血清型，血清型XVI和 其混合应变。 一种制备莱米酵母疫苗的方法包括以下步骤：（a）培养从吸吮物分离的烟草雷蒙酵母; （b）将灭活剂如0.3％正规化生理盐水溶液（FPSS）加入到从步骤（a）获得的培养溶液中以灭活烟草雷蒙酵母; 和（c）向灭活的雷神雷公藤培养液中加入免疫增强剂以制备混合疫苗。 此外，免疫增强剂是ISA70。
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