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公开(公告)号：KR101566376B1
公开(公告)日：2015-11-09
申请号：KR1020120156224
申请日：2012-12-28
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본발명은닭에서전염성활막염과호흡기증상을유발하는주요병원성세균인마이코플라즈마시노비에() 국내분리주에관한것으로, 보다구체적으로는국내사육닭에서분리되고, 높은전염성및 면역원성을갖는분리주중MS-09-AR 백신주(기탁번호 : KCTC12342BP)에관한것이다.
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公开(公告)号：KR101073984B1
公开(公告)日：2011-10-21
申请号：KR1020080067171
申请日：2008-07-10
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본발명은면역크로마토그라피를이용한광견병항체신속검출진단키트및 면역크로마토그라피를이용한이용한광견병항체진단방법에관한것으로보다상세하게는백킹카드(backing card) 위에검사선(test line)과대조선(control line)을포함하는니트로셀룰로오스멤브레인(nitrocellulose membrane)이구비되고; 상기니트로셀룰로오스멤브레인의일측에는콘쥬게이트패드(conjugate pad)가중첩되어형성되는한편, 상기콘쥬게이트패드위에혈청시료를적하하는샘플패드(smaple pad)가중첩되어구비되며; 상기니트로셀룰로오스멤브레인의타측에는백킹카드위에반응이종료된혈청을흡수하는흡수패드(adsorbent pad)가구비되는면역크로마토그라피를이용한광견병항체신속검출진단키트및 면역크로마토그라피를이용한광견병항체진단방법에관한것이다.
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公开(公告)号：KR1020090039230A
公开(公告)日：2009-04-22
申请号：KR1020070104736
申请日：2007-10-17
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N7/00
Abstract: A new vaccine to duck hepatitis virus is provided to have excellence in resistance against epidemic duck hepatitis and lessen damage caused by the duck hepatitis. A method for producing vaccine(deposition No. KCCM10883P) of duck hepatitis virus having sequence of sequence No. 1 [SEQ ID NO:1] comprises: a step of diluting recently epidemic duck hepatitis virus in phosphate buffer; a step of inoculating 7-9 days old chicken embryo; a step of culturing the inoculated chicken embryo at 37°C for three days; and a step of collecting the cultured chicken embryo and performing subculture sequencially to adapt the chicken embryo. A process for manufacturing a new vaccine is performed with: the step of attenuating the duck hepatitis virus; a step of testing restoration of attenuated virus; a step of searching defense ability of vaccine to selected duck hepatitis virus; a step of searching defense ability in each pathway; a step of searching minimum amount of the vaccine; and a step of safety and amount of the vaccine.
Abstract translation: 提供了一种新型的鸭肝炎病毒疫苗，具有卓越的抗流行性鸭肝炎防治能力，减轻了鸭肝炎所造成的伤害。 具有序列号1 [SEQ ID NO：1]序列的鸭肝炎病毒疫苗（沉积编号KCCM10883P）的制备方法包括：在磷酸盐缓冲液中稀释近期流行的鸭肝炎病毒的步骤; 接种7-9天龄鸡胚的步骤; 将接种的鸡胚在37℃培养3天的步骤; 并收集培养的鸡胚并顺序进行亚文化以适应鸡胚的步骤。 制备新疫苗的方法是：减毒鸭肝炎病毒的步骤; 测试减毒病毒恢复的一个步骤; 检测疫苗对选择的鸭肝炎病毒的防御能力的一个步骤; 在每个途径中寻找防御能力的一个步骤; 搜索最少量的疫苗的步骤; 和疫苗的安全和数量的步骤。
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公开(公告)号：KR1020110044427A
公开(公告)日：2011-04-29
申请号：KR1020090101085
申请日：2009-10-23
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: Y02A50/451 , C12Q1/6888 , C12N15/11 , C12Q1/6844
Abstract: PURPOSE: A method for detecting Salmonella gallinarum or probiotics for Salmonella gallinarum vaccine is provided to quickly detect Salmonella gallinarum. CONSTITUTION: A method for detecting Salmonella gallinarum or probitocs Salmonella gallinarum comprises: a step of preparing DNA from a biological sample; a step of performing PCR using the DNA as a temperate and primer; and a step of analyzing DNA fragment through from PCR product.
Abstract translation: 目的：提供一种检测沙门氏菌沙门氏菌或益生菌沙门氏菌疫苗的方法，以快速检测沙门氏菌。 构成：检测沙门氏菌或前列腺素沙门氏菌的方法包括：从生物样品制备DNA的步骤; 使用DNA作为温带和引物进行PCR的步骤; 以及通过PCR产物分析DNA片段的步骤。
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Patent Agency Ranking
公开(公告)号：KR1020160056209A
公开(公告)日：2016-05-19
申请号：KR1020140156338
申请日：2014-11-11
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12Q1/68
CPC classification number: Y02A50/451 , C12Q1/689 , C12Q2527/101 , C12Q2547/101 , C12Q2549/119
Abstract: 본발명은클로스트리디움보툴리눔()의독소단백질C의경쇄영역코딩유전자의중합효소연쇄반응용프라이머세트, 이를포함하는보툴리눔독소형 C/D 및 C 탐지용조성물, 및탐지용키트에 관한것으로, 본발명에따른클로스트리디움보툴리눔() 독소형 C/D 와 C의독소단백질의 N 말단을코딩하는유전자에공통적으로결합하는프라이머세트및 비특이반응억제용올리고뉴클레오티드를이용하여단일튜브이중중합효소연쇄반응을수행할경우, 보툴리눔에대한민감도가개선되어시료를대상으로하여더 낮은검출한계를보임으로서증균과정없이수행할수 있기에신속한진단이가능하다.
Abstract translation: 本发明涉及一种用于C型肉毒毒素轻链区编码基因的聚合酶链反应引物组，用于检测C型肉毒杆菌的组合物和包含该引物组的C型 检测试剂盒包括引物组。 当使用本发明的引物组进行单管双重聚合酶链反应时，其中与编码肉毒杆菌毒素C / D型和C蛋白的N末端的基因与寡核苷酸两者结合的引物组 抑制非特异性反应，可以提高对肉毒杆菌的敏感性，并且可以降低检测阈值，因此可以在没有富集过程的情况下快速进行检测。
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公开(公告)号：KR101149875B1
公开(公告)日：2012-06-27
申请号：KR1020090101085
申请日：2009-10-23
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: Y02A50/451
Abstract: PURPOSE: A method for detecting Salmonella gallinarum or probiotics for Salmonella gallinarum vaccine is provided to quickly detect Salmonella gallinarum. CONSTITUTION: A method for detecting Salmonella gallinarum or probitocs Salmonella gallinarum comprises: a step of preparing DNA from a biological sample; a step of performing PCR using the DNA as a temperate and primer; and a step of analyzing DNA fragment through from PCR product.
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公开(公告)号：KR100959259B1
公开(公告)日：2010-05-26
申请号：KR1020070104736
申请日：2007-10-17
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N7/00
Abstract: 본 발명은 새로운 오리 간염 바이러스 백신주에 관한 것으로서, 보다 상세하게는 최근 국내에서 유행하는 오리 간염바이러스를 인산완충액에 희석하여 7-9일령 계태아에 접종하여 37℃에서 3일간 배양한 뒤 계태아를 채취하여 다시 인산완충액으로 유제하여 연속으로 계태아에 계대하여 순응시켜 제조한 오리 간염 바이러스 백신주에 관한 것이다. 본 발명에 의한 신규 오리 간염바이러스 백신은 1일령 어린 오리에서 접종할 경우 병원성이 관찰되지 않고 최근 국내에서 유행하는 오리 간염에 대해 100%의 방어능을 발휘할 수 있었다.
오리 간염바이러스 1형, 최근 국내유행 오리 간염바이러스, 백신주, 병원성, 방어능-
公开(公告)号：KR1020100006868A
公开(公告)日：2010-01-22
申请号：KR1020080067171
申请日：2008-07-10
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A diagnostic kit for rapid detection of rabies antibodies is provided to rapidly detect the existence according to rabies vaccination within 20 minutes without expensive equipment and to reduce the generation of rabies. CONSTITUTION: A diagnostic kit for rapid detection of rabies antibodies using immunochromatography has a nitrocellulose membrane(30) containing a test line(40) and a control line(50) on a backing card(70). A conjugate pad is overlapped at one side of the nitrocellulose membrane. A sample pad(10) is overlapped on the conjugate pad. An adsorbent pad(60) absorbing blood serum in which the reaction is completed on a backing card is installed at the other side of the nitrocellulose membrane.
Abstract translation: 目的：提供用于快速检测狂犬病抗体的诊断试剂盒，用于在20分钟内快速检测狂犬病疫苗的存在，无需昂贵的设备，并减少狂犬病的产生。 构成：用于使用免疫层析法快速检测狂犬病抗体的诊断试剂盒具有在背板（70）上含有测试线（40）和对照线（50）的硝酸纤维素膜（30）。 缀合垫在硝酸纤维素膜的一侧重叠。 样品垫（10）重叠在共轭垫上。 在硝酸纤维素膜的另一侧安装吸收血清的吸附垫（60），其中在背衬卡上完成反应。
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公开(公告)号：KR1020090114665A
公开(公告)日：2009-11-04
申请号：KR1020080040422
申请日：2008-04-30
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: G01N33/48 , G01N33/52 , G01N33/543
Abstract: PURPOSE: A diagnostic latex liquid and a simple method for diagnosing laptospira are provided to quickly confirm infection of laptospira and minimize the infection. CONSTITUTION: A diagnostic latex liquid using laptospira serum sejroe is prepared by crushing the laptospira serum sejroe by ultrasonic wave and adhering to latex bead. An antigen used for latex diagnosis is laptospira serum sejroe. The crushing is performed at 10-60 KHz for 10 to 30 minutes. A method for diagnosing laptospira comprises: a step of mixing same amount of test serum with 30ul of latex liquid on glass plate; a step of shaking the glass plate and read formation of aggregation; and a step of diagnosing that the formation of specific aggregation is laptospira positive and no aggregation is laptospira negative.
Abstract translation: 目的：提供诊断乳液液体和简单的诊断Laptospira方法，以快速确认laptospira感染并尽量减少感染。 构成：使用laptospira血清sejroe的诊断胶乳液通过超声波粉碎laptospira血清sejroe并粘附到乳胶珠制备。 用于乳胶诊断的抗原是laptospira血清sejroe。 破碎在10-60 KHz进行10〜30分钟。 一种诊断眼线笔的方法包括：将相同量的血清与30ul胶乳液混合在玻璃板上的步骤; 摇晃玻璃板并阅读聚集的步骤; 诊断特定聚集体形成为laptospira阳性并且没有聚集是laptospira阴性的步骤。
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