Abstract:
The present invention relates to a a polymerase chain reaction (PCR) primer for detecting pectobacterium carotovorum subsp. carotovorum and a method for detecting the pectobacterium carotovorum subsp. carotovorum using the same. A user can detect the pectobacterium carotovorum subsp. carotovorum immediately if the user uses the PCR primer according to the present invention an follows the conditions of gene amplification.
Abstract:
PURPOSE: A method for selecting a gene from a microarray is provided to easily detect a novel gene which is not classified through a conventional method. CONSTITUTION: A method for screening a candidate gene through microarray data analysis comprises: a step of measuring expression intensity from each spot of microarray; a step of calculating expression intensity rate and standard value through maximum boundary from value data(absolute value of the expression intensity); and a step of comparing absolute value, and standard value to expression intensity rate to select candidate gene. An analysis apparatus of nucleic acid or protein microarray comprises: a detection unit which measures expression intensity from each spot of microarray; a calculation unit which calculates expression intensity rate and standard value; a database which storing expression intensity rate and standard value; a selection part which chooses candidate genes; and an output unit which outputs value data, selected candidate gene, expression intensity rate and standard value.
Abstract:
The present invention relates to Pseudomonas aeruginosa specific primers and a method for detecting Pseudomonas aeruginosa using the same, and more specifically to a method for detecting and diagnosing Pseudomonas aeruginosa species specifically without extracting DNA from a sample by using a pair of primers combining to O-antigen acetylase genes of Pseudomonas aeruginosa.
Abstract:
본 발명은 벼잎줄무늬병원균의 검출용 프라이머 세트, 이를 이용한 벼잎줄무늬병원균의 검출방법, 이를 포함하는 벼잎줄무늬병원균 검출용 키트에 관한 것이다. 본 발명에 따른 프라이머 세트를 이용하면 벼잎줄무늬병원균을 신속하고 정확하게 검출할 수 있어 벼의 세균병의 감염 여부를 효과적으로 진단할 수 있으므로, 벼줄무늬잎마름병을 예방하고 방제하는데 기여할 수 있다.
Abstract:
본 발명은 벼의 세균병원균인 버크홀데리아 글루메( Burkholderia glumae )의 검출용 프라이머 세트, 이를 이용한 버크홀데리아 글루메의 검출방법, 이를 포함하는 버크홀데이라 글루메 검출용 키트에 관한 것이다. 본 발명에 따른 프라이머 세트를 이용하면 벼의 세균병원균인 버크홀데리아 글루메( Burkholderia
glumae )를 신속하고 정확하게 검출할 수 있어 벼의 세균병의 감염 여부를 효과적으로 진단할 수 있으므로, 벼의 세균병을 예방하고 방제하는데 기여할 수 있다.
Abstract:
PURPOSE: A primer set for detecting Clavibacter michiganensis subsp. Sepedonicus is provided to quickly and accurately detect the bacteria and to prevent ring rot in potatoes. CONSTITUTION: A primer set for detecting Clavibacter michiganensis subsp. Sepedonicus contains primers of sequence numbers 1 and 2. A method for detecting Clavibacter michiganensis subsp. Sepedonicus comprises a step of amplifying DNA isolated from a plant sample using the primer set. A kit for detecting Clavibacter michiganensis subsp. Sepedonicus contains the primer set.
Abstract:
PURPOSE: A wound stress-induced promoter is provided to enhance the harvest amount of various crops and to produce commercial crops without environmental harm. CONSTITUTION: A wound stress-induced promoter derived from Oryza sativa has a base sequence of sequence number 1. The promoter contains a recombinant plant expression vector. The recombinant plant expression vector is prepared by operatively linking a target gene encoding a target protein at downstream of the promoter. The target protein is a plant disease resistant protein, a high functional protein, a cell regulatory protein, an immunomodulatory protein, an inhibitory protein, or an enzyme. A method for producing a transgenic plant comprises: a step of transforming plant cells with the recombinant plant expression vector; and a step of redifferentiating the transgenic plant.
Abstract:
본 발명은 벼잎줄무늬병원균 또는 벼흰잎마름병원균 검출용 프라이머 쌍 및 이를 이용한 멀티플렉스 PCR 방법에 관한 것으로, 본 발명의 프라이머 쌍은 벼잎줄무늬병원균 또는 벼흰잎마름병원균의 특이 서열과 관련되어, 신속, 정확하게 벼잎줄무늬병원균 또는 벼흰잎마름병원균을 검출하는데 유용하게 이용될 수 있으며, 또한 이를 이용한 멀티플렉스 PCR 방법은 식물체가 벼잎줄무늬병원균 및 벼흰잎마름병원균에 감염 되었는지 여부를 동시에 판별하는데 유용하게 이용될 수 있다. 벼잎줄무늬병원균, 벼흰잎마름병원균, 프라이머, 멀티플렉스
Abstract:
A method for detecting a Pseudomonas syringae pv. phaseolicola is provided to quickly and accurately determine the infection of Pseudomonas syringae pv. phaseolicola using a probe and primer pair related to a Pseudomonas syringae pv. phaseolicola specific sequence. A probe for detecting a Pseudomonas syringae pv. phaseolicola comprises a sequence containing 22th and 828th bases of the sequence number 1(SEQ ID NO:1) or complementary sequence of the sequence. A microarray for detecting Pseudomonas syringae pv. phaseolicola comprises a substrate containing a probe on the surface. A primer pair for detecting Pseudomonas syringae pv. phaseolicola comprises sequences of the sequence numbers 2 and 3. The Pseudomonas syringae pv. phaseolicola is detected by PCR analysis using the probe or primer pair.