Abstract:
PURPOSE: A wound stress-induced promoter is provided to enhance the harvest amount of various crops and to produce commercial crops without environmental harm. CONSTITUTION: A wound stress-induced promoter derived from Oryza sativa has a base sequence of sequence number 1. The promoter contains a recombinant plant expression vector. The recombinant plant expression vector is prepared by operatively linking a target gene encoding a target protein at downstream of the promoter. The target protein is a plant disease resistant protein, a high functional protein, a cell regulatory protein, an immunomodulatory protein, an inhibitory protein, or an enzyme. A method for producing a transgenic plant comprises: a step of transforming plant cells with the recombinant plant expression vector; and a step of redifferentiating the transgenic plant.
Abstract:
본발명은남평벼유래 DNA 마커를포함하는키다리병저항성벼 품종선별용조성물에관한것으로, 보다상세하게는 1625IND에대한서열번호 249 및 250으로표시되는프라이머쌍, 및 1675IND에대한서열번호 251 및 252로표시되는프라이머쌍으로이루어진군으로부터선택되는하나이상의프라이머쌍을포함하는, 키다리병저항성벼 품종선별용조성물에관한것이다. 또한, 상기조성물을포함하는키다리병저항성벼 품종선별키트, 상기조성물을이용한키다리병저항성벼 품종선별방법, 및키다리병저항성벼 품종생산방법에관한것이다. 한편, 본발명은키다리병저항성과관련된양적형질유전자좌(QTLs, quantitative trait loci)를결정하기위한유전지도작성용 CAPS(cleaved amplified polymorphic sequences) 마커증폭용프라이머세트에관한것이다. 본발명에따른남평벼유래 DNA 마커를포함하는키다리병저항성벼 품종선별용조성물을이용하여효율적으로키다리병저항성및 감수성개체를구분할수 있으며, 본발명의 DNA 마커는 MAS(Marker Assisted Selection)를통한키다리병저항성을개선한벼 육종에유용하게사용될수 있다. 이에, 벼키다리병저항성품종개발촉진으로인해키다리병에대한피해절감및 농약사용량또한절감할수 있다.