Abstract:
A method of multiplex PCR using a primer set for detecting Xanthomonas oryzae pv. oryzicola or Xanthomonas oryzae pv. oryzae is provided to quickly and accurately infection by the paphogens. A primer set for detecting a Xanthomonas oryzae pv. oryzicola is denoted by the sequence number 3(SEQ ID NO:3) and 4. A primer set for detecting a Xanthomonas oryzae pv. oryzae is denoted by the sequence numbers 5 and 6. A multiplex PCR method for detecting Xanthomonas oryzae pv. oryzicola and Xanthomonas oryzae pv. oryzae is performed by using primer set of the sequence numbers 5 and 6.
Abstract:
A method for manufacturing intron based polymorphism primer set through in-silico analysis is provided to quickly and massively produce molecular marker and develop novel species. A method for manufacturing an intro based polymorphism primer set comprises: a step of comparing gene of Brassicaceae plant with expressed gene sequence tag to distinguish exon and intron in the plant gene; a step of determining common axon; and a step of performing in-silico simulation using intron between the axons to produce the intron based polymorphism primer. The Brassicaceae plant is Chinese cabbage, Brassica oleracea var. or broccoli.
Abstract:
본 발명은 풋마름병원균( Ralstonia solanacearum )의 특이 DNA 단편 검출을 위한 합성 올리고뉴클레오티드 및 이들을 이용한 풋마름병 병원균에 감염된 식물체의 검출 방법에 관한 것으로, 보다 상세하게는 풋마름병원균의 특이 DNA 단편을 검출할 수 있는 20mer로 제작된 한 쌍의 합성 올리고뉴클레오티드를 이용하여 풋마름병원균의 감염 여부를 신속, 정확하게 진단할 수 있는 종(species) 특이적인 합성 올리고뉴클레오티드에 관한 것이다. 풋마름병원균, bacterial wilt, Ralstonia solanacearum, 특이 DNA 단편, 합성 올리고뉴클레오티드, 검출, 진단
Abstract:
본 발명은 벼잎줄무늬병원균 또는 벼흰잎마름병원균 검출용 프라이머 쌍 및 이를 이용한 멀티플렉스 PCR 방법에 관한 것으로, 본 발명의 프라이머 쌍은 벼잎줄무늬병원균 또는 벼흰잎마름병원균의 특이 서열과 관련되어, 신속, 정확하게 벼잎줄무늬병원균 또는 벼흰잎마름병원균을 검출하는데 유용하게 이용될 수 있으며, 또한 이를 이용한 멀티플렉스 PCR 방법은 식물체가 벼잎줄무늬병원균 및 벼흰잎마름병원균에 감염 되었는지 여부를 동시에 판별하는데 유용하게 이용될 수 있다. 벼잎줄무늬병원균, 벼흰잎마름병원균, 프라이머, 멀티플렉스
Abstract:
A method for detecting a Pseudomonas syringae pv. phaseolicola is provided to quickly and accurately determine the infection of Pseudomonas syringae pv. phaseolicola using a probe and primer pair related to a Pseudomonas syringae pv. phaseolicola specific sequence. A probe for detecting a Pseudomonas syringae pv. phaseolicola comprises a sequence containing 22th and 828th bases of the sequence number 1(SEQ ID NO:1) or complementary sequence of the sequence. A microarray for detecting Pseudomonas syringae pv. phaseolicola comprises a substrate containing a probe on the surface. A primer pair for detecting Pseudomonas syringae pv. phaseolicola comprises sequences of the sequence numbers 2 and 3. The Pseudomonas syringae pv. phaseolicola is detected by PCR analysis using the probe or primer pair.
Abstract:
PURPOSE: A method for selecting a gene from a microarray is provided to easily detect a novel gene which is not classified through a conventional method. CONSTITUTION: A method for screening a candidate gene through microarray data analysis comprises: a step of measuring expression intensity from each spot of microarray; a step of calculating expression intensity rate and standard value through maximum boundary from value data(absolute value of the expression intensity); and a step of comparing absolute value, and standard value to expression intensity rate to select candidate gene. An analysis apparatus of nucleic acid or protein microarray comprises: a detection unit which measures expression intensity from each spot of microarray; a calculation unit which calculates expression intensity rate and standard value; a database which storing expression intensity rate and standard value; a selection part which chooses candidate genes; and an output unit which outputs value data, selected candidate gene, expression intensity rate and standard value.
Abstract:
A synthesis oligonucleotide is provided to diagnose quickly and conveniently whether being infected with Ralstonia solanacearum or not by performing the PCR amplification and to detect the Ralstonia solanacearum specific DNA fragment. A synthesis oligonucleotide has base sequences of a sequence number 2 and a sequence number 3. The synthesis oligonucleotide Is specific to No. 1~1,341 bp or 21~952 bp of the cytochrome c1 signal peptide of the Ralstonia solanacearum which is the Ralstonia solanacearum having the base sequence 1.