公开(公告)号：KR1020100110524A

公开(公告)日：2010-10-13

申请号：KR1020090028897

申请日：2009-04-03

Applicant: 대한민국(농촌진흥청장)

Inventor： 김행훈 ,  노나영 ,  고호철 ,  조규택 ,  조은기 ,  이윤걸 ,  신동진

IPC: A23B7/055 ,  A23B7/024 ,  A01N3/00

CPC classification number: A23B7/055 ,  A01N3/00 ,  A23B7/024

Abstract: PURPOSE: A vitrification solution and loading solution are provided to enhance high usage and to improve viability and regeneration rate. CONSTITUTION: A vitrification solution contains 42.5 of glycerol, 15 of dimethylsulfoxide, 15 of ethylene glycol, and 17.5 of sucrose; 37.5 of glycerol, 15 of dimethylsulfoxide, 15 of ethylene glycol, and 22.5 of sucrose; 32.5 of glycerol, 15 of dimethylsulfoxide, 15 of ethylene glycol, and 27.5 of sucrose; and 35.0 of glycerol, 15 of dimethylsolfoxide, 15 of ethylene glycol, and 20.0 of sucrose (%, w/v). A loading solution contains 9.2 of 1.0 M glycerol, 23.9 of 0.70M of sucrose, 18.4 of 2.0 M glycerol and 22.25 of 0.65M sucrose; and 15.0 of 1.63M glycerol, 15.0 of 0.44M sucrose, 17.5 of 1.9M glycerol, and 17.5 of 0.51M of sucrose.

Abstract translation: 目的：提供玻璃化溶液和加载溶液，以提高高使用率和改善活力和再生率。 构成：玻璃化溶液含有42.5份甘油，15份二甲基亚砜，15份乙二醇和17.5份蔗糖; 37.5份甘油，15份二甲基亚砜，15份乙二醇和22.5份蔗糖; 32.5的甘油，15的二甲基亚砜，15的乙二醇和27.5的蔗糖; 和35.0的甘油，15的二甲基氧化物，15的乙二醇和20.0的蔗糖（％，w / v）。 加载溶液含有9.2的1.0M甘油，23.9的0.7M的蔗糖，18.4的2.0M甘油和22.25的0.65M蔗糖; 和15.0的1.63M甘油，15.0的0.44M蔗糖，17.5的1.9M甘油和17.5的0.51M的蔗糖。

公开(公告)号：KR1020100110525A

公开(公告)日：2010-10-13

申请号：KR1020090028899

申请日：2009-04-03

Applicant: 대한민국(농촌진흥청장)

Inventor： 김행훈 ,  노나영 ,  나영왕 ,  김정봉 ,  백형진 ,  조은기 ,  이윤걸

IPC: A23B7/055 ,  A23B7/045 ,  A23B7/024 ,  A01N3/00

CPC classification number: A23B7/055 ,  A01N3/00 ,  A23B7/024 ,  A23B7/045

Abstract: PURPOSE: A cryopreservation and regeneration method for Chrysanthemum shoot is provided to preserve Chrysanthemum using vitrification solution and loading solution. CONSTITUTION: A cryopreservation for cryopreservation shoot comprises: a step of extracting laterial bud of in vitro cultured cryopreservation; a step of pre-culturing in 0.3M-0.5M-0.7M of sucrose medium; a step of culturing in a loading solution(17.5% of glycerol and 17.5% of sucrose) for 40-50 minutes; a step of shaking and dehydrating the Chrysanthemum; and a step of rapidly-freezing in the liquid nitrogen.

Abstract translation: 目的：提供菊花芽的冷冻保存和再生方法，用玻璃化溶液和加载溶液保存菊花。 构成：冷冻保存芽的冷冻保存包括：提取体外培养的低温保存的材料芽的步骤; 在0.3M-0.5M-0.7M蔗糖培养基中预培养的步骤; 在负载溶液（17.5％甘油和17.5％蔗糖）中培养40-50分钟的步骤; 菊花摇摆脱水的一步; 以及在液氮中快速冷冻的步骤。