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公开(公告)号：KR1020140065753A
公开(公告)日：2014-05-30
申请号：KR1020120132317
申请日：2012-11-21
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6827 , C12Q2525/151 , C12Q2600/156
Abstract: The present invention relates to an SSR primer isolated from Vicia sativa spp.sativa and a use thereof. In particular, the present invention relates to an SSR primer pair with two base sequences selected among sequences of sequence numbers 1-94, a detection method of genetic polymorphism of Vicia sativa spp. sativa and an identification method of genetic resources which includes PCR using the same.
Abstract translation: 本发明涉及从蚕豆豌豆分离的SSR引物及其用途。 特别地，本发明涉及一种SSR引物对，其具有选自序列号1-94的序列中的两个碱基序列，蚕豆属遗传多态性的检测方法。 和遗传资源鉴定方法，其中包括使用该遗传资源的PCR。
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公开(公告)号：KR1020100110525A
公开(公告)日：2010-10-13
申请号：KR1020090028899
申请日：2009-04-03
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A cryopreservation and regeneration method for Chrysanthemum shoot is provided to preserve Chrysanthemum using vitrification solution and loading solution. CONSTITUTION: A cryopreservation for cryopreservation shoot comprises: a step of extracting laterial bud of in vitro cultured cryopreservation; a step of pre-culturing in 0.3M-0.5M-0.7M of sucrose medium; a step of culturing in a loading solution(17.5% of glycerol and 17.5% of sucrose) for 40-50 minutes; a step of shaking and dehydrating the Chrysanthemum; and a step of rapidly-freezing in the liquid nitrogen.
Abstract translation: 目的：提供菊花芽的冷冻保存和再生方法，用玻璃化溶液和加载溶液保存菊花。 构成：冷冻保存芽的冷冻保存包括：提取体外培养的低温保存的材料芽的步骤; 在0.3M-0.5M-0.7M蔗糖培养基中预培养的步骤; 在负载溶液（17.5％甘油和17.5％蔗糖）中培养40-50分钟的步骤; 菊花摇摆脱水的一步; 以及在液氮中快速冷冻的步骤。
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公开(公告)号：KR101480155B1
公开(公告)日：2015-01-09
申请号：KR1020120132317
申请日：2012-11-21
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 베치(
Vicia sativa spp.
sativa )에서 분리한 SSR 프라이머 및 이의 용도에 관한 것으로서, 보다 상세하게는 서열번호 1 내지 서열번호 94로 이루어진 군에서 선택되는 2개의 염기서열을 갖는 SSR 프라이머쌍 및 이를 이용하여 PCR을 수행하는 것을 포함하는 베치의 유전적 다형성 검출 방법 및 유전자원의 동정 방법에 관한 것이다.
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