Abstract:
The present invention relates to a primer for detecting clubroot in Chinese cabbage and a detection method using the same. By using the detection method according to the present invention, Plasmodiophora brassicae can be specifically and simply detected.
Abstract:
PURPOSE: A red pepper Phytophthora capsici species classification primer is provided to quickly and accurately diagnose the red pepper and soil infected to Phytophthora capsici by specifically diagnosing on Phytophthora capsici generated on the red pepper. CONSTITUTION: A red pepper Phytophthora capsici species classifying kit comprises a primer set specifically recognizing internal transcribe spacer (ITS) genetic of Phytophthora capsici. The ITS genetic of Phytophthora capsici is any one of the sequcne number 3 to 9. The species classification method of red pepper Phytophthora capsici comprises a step fo preparing a sample by collecting the sample soil, and a step of performing PCR in primer set of sequence number 2 for reverse primer, and a forward primer with sequence number 1. The red pepper Phytophthora capsici species classifying PCR primer set comprises the sequence number 1and 2.
Abstract:
A method for sterilizing diseases and insect pests in soils and used mediums caused by a drench by using high temperature water of a boiler is provided to prevent environmental pollution, improve productivity and sharply reduce replacement cost of infected mediums such as rock wool and pearlite by uniformly spraying and supplying high temperature water generated from an existing sterilization boiler for high temperature water into a predetermined depth of soils. A method for sterilizing diseases and insect pests in soils and used mediums caused by a drench by using high temperature water of a boiler comprises the steps of: flattening the surface of the medium after performing rotary operation of a sterilization medium with a predetermined area; disposing a plurality of heat resistant spray hoses with a predetermined length on the surface of the sterilization medium such that the spray hoses are spaced from one another in a predetermined distance, and respectively connecting the spray hoses to distribution part such as distribution heads for drench; piping the distribution part to a boiler for supplying high temperature water; covering vinyl on an upper part of the entire area of the heat resistant spray hose installed sterilization medium; maintaining an internal temperature of a cultivation layer with a depth of 30 cm from the surface of the sterilization medium to 65 deg.C or more by allowing the vinyl to maintain residual heat for a long period of time while consistently spraying and supplying high temperature water that is heated by the boiler and maintains a predetermined temperature onto the sterilization medium for a predetermined time; and removing the vinyl, the spray hoses and the distribution headers with the passage of a predetermined time.