Abstract:
PURPOSE: A transgenic chicken and a method for producing the same are provided to ensure anti-viral ability and to be used as a model animal for preventing viral diseases. CONSTITUTION: A vector for transformation contains a promoter which is operable in birds and a 3D8 scFv gene which is operatively linked to the promoter. The promoter is a chicken beta-actin gene promoter. The 3D8 scFv gene further contains a reporter gene. The reporter gene is HA-tag. A method for producing a transgenic chicken comprises: a step of preparing the vector; a step of transducing the vector to chicken fertilized eggs; and a step of hatching the fertilized eggs.
Abstract:
PURPOSE: A method and an apparatus for automatically controlling seeding and fertilizing amount are provided to improve seeding and fertilizing accuracy. CONSTITUTION: A method for automatically controlling seeding and fertilizing amount is as follows. The speed of a tractor is measured by using a GPS sensor(110). The speed of the tractor is obtained. The ascending and descending availability of a seed metering unit(120) equipped with a seed metering roller is determined. The speed of the tractor is measured by using the GPS sensor when the ascending and descending of the seed metering unit is impossible. A drive motor is driven according to the speed of the drive motor when the ascending and descending of the seed metering unit is possible.
Abstract:
본 발명은 닭에서의 면역원성이 감소된 재조합 3D8 scFv(single chain variable fragment) 항체 및 이의 항원 결합부에 관한 것이다. 또한, 본 발명은 상기의 항체 및 이의 항원 결합부를 암호화하는 핵산 분자와 상기의 항체 및/또는 이의 항원 결합부를 포함하는 조성물에 관한 것이다. 본 발명에 따르면 닭에 쥐 유래 3D8 scFv 항체를 주입할 경우 발생할 수 있는 HAMA 유사 현상 등을 유발하지 않도록 면역원성이 감소되며, 핵산결합능, 핵산분해능 및 세포 침투능 등과 같은 기존 3D8 scFv 항체의 활성을 유지하는 재조합 항체가 제공된다.
Abstract:
A primer for diagnosing potato virus is provided to diagnose specifically 4 kinds of potato viruses such as potato virus Y(PVY), potato leafroll virus(PLRV), potato virus X(PVX) and potato virus S(PVS) at the same time through one RT-PCR reaction, thereby saving cost and labor force of the diagnosis. A primer for diagnosing PVY, PLRV, PVX and PVS simultaneously consists of primers of SEQ ID : NOs. 1 to 8. A method for diagnosing potato virus infection comprises the steps of: (a) isolating nucleic acid from a potato infected or suspicious to be infected by at least one of the PVY, PLRV, PVX and PVS; and (b) subjecting the isolated nucleic acid to RT-PCR using the primers of SEQ ID : NOs. 1 to 8, where the RT-PCR is done by reverse-transcribing it at a temperature of 45 deg.C for 30 minutes, removing reverse transcriptase therefrom at a temperature of 94 deg.C for 4 minutes, repeating a reaction 35 times, the reaction consisting of denaturing for 30 seconds at a temperature of 94 deg.C, annealing for 30 seconds at a temperature of 50 deg.C and elongating for 60 seconds at a temperature of 72 deg.C, treating it at a temperature of 72 deg.C for 5 minutes and maintaining the temperature at 4 deg.C. Further, a concentration ratio of the SEQ ID : NOs. 1: the SEQ ID : NOs. 2: the SEQ ID : NOs. 3: the SEQ ID : NOs. 4 is10:10:1:3.
Abstract translation:提供用于诊断马铃薯病毒的引物,用于同时诊断马铃薯病毒Y(PVY),马铃薯卷叶病毒(PLRV),马铃薯病毒X(PVX)和马铃薯病毒S(PVS)等4种马铃薯病毒, 一次RT-PCR反应,从而节省诊断成本和劳动力。 用于诊断PVY,PLRV,PVX和PVS的引物同时由SEQ ID NO:的引物组成。 用于诊断马铃薯病毒感染的方法包括以下步骤:(a)从感染或可疑的马铃薯中分离核酸以被PVY,PLRV,PVX和PVS中的至少一种感染; 和(b)使用SEQ ID NO:的引物对分离的核酸进行RT-PCR。 1〜8,RT-PCR在45℃的温度下反转录30分钟,在94℃的温度下除去逆转录酶4分钟,重复反应35次, 该反应在94℃的温度下变性30秒,在50℃的温度退火30秒,在72℃的温度下延伸60秒,在72℃的温度下处理 5℃,保持温度在4℃。 此外,SEQ ID NO: 1:SEQ ID:NO。 2:SEQ ID:NO。 3:SEQ ID:NO。 4是10:10:1:3。
Abstract:
본 발명은 감자에 발생하는 주요 바이러스와 비특이적 반응이 일어나지 않고, 감자S바이러스( Potato virus S , PVS)에 유일하게 존재하는 DNA(유전자) 염기서열(부분)에만 대응하는 특이한 프라이머 세트(PVSCP5와 PVSCP3)를 제조하는 것에 관한 것이다. 이를 이용하여 감자S바이러스를 진단할 수 있을 뿐만 아니라 감자바이러스를 예방할 수 있다.