公开(公告)号：KR101004753B1

公开(公告)日：2011-01-07

申请号：KR1020100044012

申请日：2010-05-11

Applicant: 대한민국 (식품의약품안전처장)

Inventor： 김철규 ,  강호일 ,  심선보 ,  지승완 ,  이수해 ,  배창준 ,  김형욱

IPC: C12N5/073 ,  C12N15/63 ,  A01K67/027

Abstract: PURPOSE: A transgenic mouse which expresses luciferase and a method for producing the same are provided to effectively detect allergen existing in an environment. CONSTITUTION: A method for screening allergen comprises: a step of treating allergen to a transgenic mouse which is produced from transgenic mouse fertilized egg(deposit number KCTC 11575BP) for detecting allergen; and a step of measuring the amount of luciferase expressed in the transgenic mouse. A method for manufacturing mouse pIL-4/Luc/CNS-1 expression cassette expressing luciferase comprises: a step of amplifying IL-4 promoter base sequence of sequence number 1 by PCR; and a step of constructing pIL-4/Luc/CNS-1 vector by binding pTransLucent vector and amplified IL-4 promoter by restriction enzymes, NheI and BglII.

Abstract translation: 目的：提供表达荧光素酶的转基因小鼠及其生产方法，以有效地检测环境中存在的过敏原。 构成：筛选过敏原的方法包括：对转基因小鼠进行变应原的步骤，该转基因小鼠由转基因小鼠受精卵（保藏号KCTC 11575BP）产生，用于检测变应原; 以及测量转基因小鼠中表达的荧光素酶的量的步骤。 用于制造表达荧光素酶的小鼠pIL-4 / Luc / CNS-1表达盒的方法包括：通过PCR扩增序列号1的IL-4启动子碱基序列的步骤; 以及通过用限制酶NheI和BglII结合pTransLucent载体和扩增的IL-4启动子来构建pIL-4 / Luc / CNS-1载体的步骤。

公开(公告)号：KR100818795B1

公开(公告)日：2008-04-03

申请号：KR1020070124198

申请日：2007-12-03

Applicant: 대한민국 (식품의약품안전처장)

Inventor： 이상목 ,  박건상 ,  김우성 ,  강호일 ,  이창희 ,  임동길 ,  고용석 ,  김소희 ,  조대현 ,  김대병 ,  유원곤

IPC: G06Q50/22 ,  G06K17/00

CPC classification number: G06Q10/087

Abstract: A system for managing reagents with RFID(Radio Frequency IDentification) is provided to perform general reagent management such as information, usage, stock, and expiration management, and manage storage location and safety of each reagent in the lab by using the RFID. A storage ID tag(100) is attached to a reagent storage(1) of a lab, and stores an ID code for identifying the reagent storage and a list of the reagents stored in the reagent storage. A reagent ID tag(200) is attached to a lid of a reagent bottle(5) stored in the reagent storage, and stores the ID code of the reagent storage for storing the reagent and information related to the reagent. A portable terminal(300) receives/outputs the ID code of the reagent storage, the reagent list, and the reagent information stored in the reagent storage ID tag and the reagent ID tag by including an RFID reader. A reagent management server(400) receives/stores information recognized by the portable terminal through the network, manages usage/storage of each reagent, issues a warning for an incorrect storage location by checking the storage location of each reagent bottle, issues the warning for emergency, and provides emergency management information.

Abstract translation: 提供用于管理RFID试剂的系统（射频识别），以进行一般试剂管理，如信息，使用，库存和到期管理，并通过使用RFID管理实验室中每种试剂的存储位置和安全性。 存储ID标签（100）附接到实验室的试剂库（1），并且存储用于识别试剂储存器的ID代码和存储在试剂储存器中的试剂列表。 将试剂ID标签（200）附着到存储在试剂库中的试剂瓶（5）的盖上，存储试剂储存用的试剂储存库的ID码和与试剂有关的信息。 便携式终端（300）通过包括RFID阅读器来接收/输出存储在试剂储存ID标签和试剂ID标签中的试剂存储的ID码，试剂列表和试剂信息。 试剂管理服务器（400）通过网络接收/存储由便携式终端识别的信息，管理每种试剂的使用/存储，通过检查每个试剂瓶的存储位置对不正确的存储位置发出警告，发出警告 紧急情况，并提供应急管理信息。