公开(公告)号：KR100321275B1

公开(公告)日：2002-05-13

申请号：KR1019980040708

申请日：1998-09-30

Applicant: 동화약품주식회사 ,  학교법인 포항공과대학교

Inventor： 윤성준 ,  김종우 ,  성영철 ,  이경재 ,  신동혁 ,  김남두 ,  최요한 ,  박상진 ,  장환봉 ,  한진희

IPC: C07K7/08

Abstract: 본 발명은 세포 수용체인 CD4와 인간 면역 결핍 바이러스-1 (human immunodeficiency virus type 1, 이하 "HIV-1"로 약칭함)의 외막 단백질인 gp120과의 상호작용을 저해하는 펩타이드, 그의 용도 및 이 펩타이드의 선별방법에 관한 것으로, 구체적으로는 12개의 아미노산으로 이루어지고 아미노 말단으로부터 4번 아미노산은 프롤린 (proline), 6번 아미노산은 페닐알라닌 (phenylalanine)이고 나머지는 임의의 아미노산인 펩타이드로서 세포 수용체인 CD4와 HIV-1의 외막 단백질인 gp120과의 상호작용을 저해하는 본 발명의 펩타이드는 그 크기가 화학적 합성이 가능한 수준으로 작으면서도 기존의 후천성 면역 결핍증 (acquired immunodeficiency syndrome, 이하 "AIDS"로 약칭함) 치료제의 작용 단계와는 전혀 다른 단계인 HIV-1 바이러스의 감염 초기 단계에 작용하여 세포 수용체인 CD4와 HIV-1의 � ��막 단백질인 gp120과의 상호작용을 특이적으로 저해하므로 새로운 AIDS 치료제로 개발될 수 있을 뿐만 아니라, 다른 AIDS 치료제와 병용되는 복합 치료 방법을 통하여 AIDS 치료에 효과적으로 사용될 수 있는 펩타이드이다. 또한, 본 발명에서는 아가로스 비드 (agarose bead)를 사용하는 특징적인 패닝방법을 사용하여 상기 펩타이드을 효과적으로 선별할 수 있다.

公开(公告)号：KR1020000021557A

公开(公告)日：2000-04-25

申请号：KR1019980040708

申请日：1998-09-30

Applicant: 동화약품주식회사 ,  학교법인 포항공과대학교

Inventor： 윤성준 ,  김종우 ,  성영철 ,  이경재 ,  신동혁 ,  김남두 ,  최요한 ,  박상진 ,  장환봉 ,  한진희

IPC: C07K7/08

CPC classification number: C07K7/08 ,  C07K7/64

Abstract: PURPOSE: Provided is a peptide inhibiting an interaction between gp120 that is outer membrane protein of HIV and a cell receptor, CD4 on a stage of cell infection of HIV CONSTITUTION: A peptide consists of 12 amino acids. each amino acid comprises especially, proline and phenylalanine on the fourth and sixth position from amino-ends respectively and random amino acids. Peptide inhibits interaction between cell receptor, CD4 and outer membrane of HIV-1, gp120 on an early step of virus infection so it can be provided as a new AIDS treatment and used for combination therapy. To select a peptide which can be used for AIDS therapy, panning method which is characterized by using agarose bead as matrix and phage-displayed peptide library, is provided. In the panning method, peptide is obtained by using phosphate-buffered saline, competitive binding, and E.coli used as a host cell of phage.

Abstract translation: 目的：提供抑制HIV的外膜蛋白的gp120与细胞受体之间的相互作用的肽，HIV在细胞感染阶段的CD4组成：肽由12个氨基酸组成。 每个氨基酸分别特别包括氨基末端的第四和第六位上的脯氨酸和苯丙氨酸以及随机氨基酸。 肽在病毒感染的早期阶段抑制细胞受体，CD4和HIV-1外膜之间的相互作用，因此可以作为新的AIDS治疗提供并用于联合治疗。 为了选择可用于AIDS治疗的肽，提供以使用琼脂糖珠作为基质和噬菌体展示肽文库的淘选方法。 在淘选方法中，通过使用磷酸盐缓冲盐水，竞争性结合和用作噬菌体宿主细胞的大肠杆菌获得肽。

公开(公告)号：KR100407817B1

公开(公告)日：2003-11-28

申请号：KR1020010052952

申请日：2001-08-30

Applicant: 학교법인 포항공과대학교

Inventor： 이승섭 ,  고상춘 ,  최요한 ,  이추희 ,  손상욱

IPC: B81B7/02

Abstract: PURPOSE: A cell number counting apparatus and a manufacturing method thereof are provided to simplify manufacturing procedures and reduce size of the apparatus by manufacturing the apparatus through the micro electro mechanical system, while achieving improved accuracy of cell number counting. CONSTITUTION: A cell number counting apparatus comprises a capillary(20) formed on a silicon substrate(80), and which serves as a passage for the liquid containing cells to be counted; an injected cell storing unit(30) formed at an end of the capillary, and which stores the liquid injected into the capillary; a discharged cell storing unit(40) formed at the other end of the capillary, and which stores the liquid discharged from the capillary; and a cell sensing unit formed in the silicon substrate and disposed beneath the capillary, and which senses the cell number by measuring the resistance changing in accordance with the quantity of the light permeation determined by the dyed degree of the cell flowing along the capillary. The capillary, injected cell storing unit, discharged cell storing unit and the cell sensing unit are manufactured through a micro electro mechanical system.

Abstract translation: 目的：提供一种单元数量计数装置及其制造方法，以通过微机电系统制造装置来简化制造过程并减小装置的尺寸，同时实现提高的单元数量计数精度。 一种细胞数量计数装置，包括：形成在硅衬底（80）上的毛细管（20），其用作含有待计数细胞的液体的通道; 注入单元存储单元（30），其形成在毛细管的一端，并且存储注入到毛细管中的液体; 放出的细胞存储单元（40），形成在毛细管的另一端，并且存储从毛细管排出的液体; 以及一个形成在硅衬底中并设置在毛细管下面的单元传感单元，它通过测量电阻的变化来检测单元数量，该电阻根据由沿着毛细管流动的单元的染色程度确定的光透过量而变化。 毛细管，注入细胞储存单元，放电细胞储存单元和细胞感测单元通过微机电系统制造。

公开(公告)号：KR1020030018698A

公开(公告)日：2003-03-06

申请号：KR1020010052952

申请日：2001-08-30

Applicant: 학교법인 포항공과대학교

Inventor： 이승섭 ,  고상춘 ,  최요한 ,  이추희 ,  손상욱

IPC: B81B7/02

CPC classification number: B81B7/02 ,  B81B1/002 ,  B81B2201/0214 ,  B81C1/00015 ,  C12M1/3407 ,  C12M23/16 ,  C12Q1/04

Abstract: PURPOSE: A cell number counting apparatus and a manufacturing method thereof are provided to simplify manufacturing procedures and reduce size of the apparatus by manufacturing the apparatus through the micro electro mechanical system, while achieving improved accuracy of cell number counting. CONSTITUTION: A cell number counting apparatus comprises a capillary(20) formed on a silicon substrate(80), and which serves as a passage for the liquid containing cells to be counted; an injected cell storing unit(30) formed at an end of the capillary, and which stores the liquid injected into the capillary; a discharged cell storing unit(40) formed at the other end of the capillary, and which stores the liquid discharged from the capillary; and a cell sensing unit formed in the silicon substrate and disposed beneath the capillary, and which senses the cell number by measuring the resistance changing in accordance with the quantity of the light permeation determined by the dyed degree of the cell flowing along the capillary. The capillary, injected cell storing unit, discharged cell storing unit and the cell sensing unit are manufactured through a micro electro mechanical system.

Abstract translation: 目的：提供细胞数量计数装置及其制造方法，以通过微机电系统制造装置来简化制造步骤并缩小装置的尺寸，同时实现提高细胞数量计数的精度。 构成：细胞数量计数装置包括在硅基板（80）上形成的毛细管（20），其作为含有细胞的液体的计数通道; 注入单元存储单元（30），其形成在毛细管的端部，并且存储注入到毛细管中的液体; 排出单元存储单元，形成在毛细管的另一端，并存储从毛细管排出的液体; 以及细胞感测单元，其形成在所述硅衬底中并且设置在所述毛细管下方，并且通过根据沿着所述毛细管流动的细胞的染色程度确定的光透过量来测量所述电阻变化来感测所述细胞数。 毛细管，注射细胞储存单元，放电单元存储单元和单元感测单元通过微机电系统制造。

公开(公告)号：KR100479848B1

公开(公告)日：2005-03-31

申请号：KR1020020080831

申请日：2002-12-17

Applicant: 학교법인 포항공과대학교

Inventor： 이승섭 ,  최요한 ,  손상욱

IPC: C12M1/34

Abstract: 본 발명은 세포 배양용 용기에 관한 것으로, 더욱 구체적으로는 기판 상에 일정한 면적을 갖는 격자 패턴을 포함하는 세포 배양용 용기에 관한 것이다. 본 발명에 의하면, 세포를 배양 용기로부터 분리하지 않고, 세포 배양용 용기로부터 직접적으로 계수할 수 있다.

公开(公告)号：KR1020040053651A

公开(公告)日：2004-06-24

申请号：KR1020020080831

申请日：2002-12-17

Applicant: 학교법인 포항공과대학교

Inventor： 이승섭 ,  최요한 ,  손상욱

IPC: C12M1/34

Abstract: PURPOSE: Cell culture dish formed with lattice is provided, thereby directly numbering cultured cells without separating the cells from the culture dish through a microscope. CONSTITUTION: The cell culture dish formed with lattice has a cell growth layer on the lattice. The method for preparing the cell culture dish formed with lattice comprises the steps of: patterning the lattice having a constant area on the metal membrane of a substrate using photolithography; and pressurizing the lattice patterned metal membrane on a substrate of the cell culture dish to copy the metal membrane lattice pattern to the substrate of the cell culture dish, wherein a compound may be coated on the lattice formed substrate of the cell culture dish to form a cell growth layer; the compound is polylysine, polyethylene, polypropylene, copolymer thereof or glass; and plasma surface modification under vacuum for proliferation of cells may be further induced.

Abstract translation: 目的：提供用格子形成的细胞培养皿，通过显微镜直接对培养细胞进行编号，而不从培养皿中分离细胞。 构成：用格子形成的细胞培养皿在晶格上具有细胞生长层。 用格子形成的细胞培养皿的制备方法包括以下步骤：使用光刻法在基片的金属膜上形成具有恒定面积的晶格; 对细胞培养皿的基板上的格子状金属膜进行加压，将金属膜格子图案复制到细胞培养皿的基板上，在细胞培养皿的格子形成的基板上涂布化合物，形成 细胞生长层; 该化合物是聚赖氨酸，聚乙烯，聚丙烯，其共聚物或玻璃; 并且可以进一步诱导在真空下进行细胞增殖的血浆表面修饰。