콜레라-유발 비브리오 콜레라에 검출용 유전자 증폭키트및 마이크로어레이
    2.
    发明公开
    콜레라-유발 비브리오 콜레라에 검출용 유전자 증폭키트및 마이크로어레이 失效
    用于检测胆固醇诱导型胆固醇的基因扩增试剂盒和微阵列

    公开(公告)号:KR1020090009999A

    公开(公告)日:2009-01-28

    申请号:KR1020070073140

    申请日:2007-07-20

    CPC classification number: Y02A50/451 Y02A50/52 C12Q1/6844 C12R1/63

    Abstract: A gene amplification kit for detecting cholera-inducing Vibrio cholera is provided to accurately and simply detect Vibrio cholera inducing cholera by analyzing the gene sequence of the certain gene of Vibrio cholera. The gene amplification kit for detecting cholera-inducing Vibrio cholera comprises the primer pair consisting of SEQ ID NO:1 and SEQ ID NO:2, the primer pair consisting of SEQ ID NO:3 and SEQ ID NO:4, the primer pair consisting of SEQ ID NO:9 and SEQ ID NO:10, the primer pair consisting of SEQ ID NO:11 and SEQ ID NO:12, the primer pair consisting of SEQ ID NO:13 and SEQ ID NO:14, the primer pair consisting of SEQ ID NO:15 and SEQ ID NO:16, the primer pair consisting of SEQ ID NO:17 and SEQ ID NO:18, the primer pair consisting of SEQ ID NO:19 and SEQ ID NO:20, the primer pair consisting of SEQ ID NO:21 and SEQ ID NO:22, or combinations thereof. A microarray for detecting cholera-inducing Vibrio cholera comprises the probe having the nucleotide sequence selected from SEQ ID NO:1 to SEQ ID NO:22.

    Abstract translation: 提供一种用于检测霍乱弧菌霍乱的基因扩增试剂盒,通过分析霍乱弧菌某些基因的基因序列,准确,简单地检测霍乱弧菌诱发霍乱。 用于检测霍乱弧菌弧菌霍乱的基因扩增试剂盒包括由SEQ ID NO:1和SEQ ID NO:2组成的引物对,由SEQ ID NO:3和SEQ ID NO:4组成的引物对,引物对由 SEQ ID NO:9和SEQ ID NO:10的引物对,由SEQ ID NO:11和SEQ ID NO:12组成的引物对,由SEQ ID NO:13和SEQ ID NO:14组成的引物对,引物对 由SEQ ID NO:15和SEQ ID NO:16组成,由SEQ ID NO:17和SEQ ID NO:18组成的引物对,由SEQ ID NO:19和SEQ ID NO:20组成的引物对,引物 由SEQ ID NO:21和SEQ ID NO:22组成的对,或其组合。 用于检测霍乱弧菌弧菌霍乱的微阵列包括具有选自SEQ ID NO:1至SEQ ID NO:22的核苷酸序列的探针。

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