公开(公告)号：KR100941990B1

公开(公告)日：2010-02-11

申请号：KR1020070073140

申请日：2007-07-20

Applicant: 서강대학교산학협력단

Inventor： 김건수 ,  김익중 ,  이규호 ,  박순정 ,  조재창 ,  양재명 ,  조유희 ,  고광표 ,  성문희

IPC: C12Q1/68

CPC classification number: Y02A50/451 ,  Y02A50/52

Abstract: 본 발명은 콜레라-유발 비브리오 콜레라에(
Vibrio

cholerae )를 검출하기 위하여 비브리오 콜레라에의 특정 유전자에 특이적으로 결합하는 프라이머 또는 프로브를 포함하는 검출용 유전자 증폭키트 또는 마이크로어레이에 관한 것이다. 본 발명에 따르면, 정확하면서도 간편하게 콜레라-유발 비브리오 콜레라에를 검출할 수 있다.
비브리오 콜레라에, Vibrio cholerae, 콜레라, 유전자 증폭키트

公开(公告)号：KR1020090009999A

公开(公告)日：2009-01-28

申请号：KR1020070073140

申请日：2007-07-20

Applicant: 서강대학교산학협력단

Inventor： 김건수 ,  김익중 ,  이규호 ,  박순정 ,  조재창 ,  양재명 ,  조유희 ,  고광표 ,  성문희

IPC: C12Q1/68

CPC classification number: Y02A50/451 ,  Y02A50/52 ,  C12Q1/6844 ,  C12R1/63

Abstract: A gene amplification kit for detecting cholera-inducing Vibrio cholera is provided to accurately and simply detect Vibrio cholera inducing cholera by analyzing the gene sequence of the certain gene of Vibrio cholera. The gene amplification kit for detecting cholera-inducing Vibrio cholera comprises the primer pair consisting of SEQ ID NO:1 and SEQ ID NO:2, the primer pair consisting of SEQ ID NO:3 and SEQ ID NO:4, the primer pair consisting of SEQ ID NO:9 and SEQ ID NO:10, the primer pair consisting of SEQ ID NO:11 and SEQ ID NO:12, the primer pair consisting of SEQ ID NO:13 and SEQ ID NO:14, the primer pair consisting of SEQ ID NO:15 and SEQ ID NO:16, the primer pair consisting of SEQ ID NO:17 and SEQ ID NO:18, the primer pair consisting of SEQ ID NO:19 and SEQ ID NO:20, the primer pair consisting of SEQ ID NO:21 and SEQ ID NO:22, or combinations thereof. A microarray for detecting cholera-inducing Vibrio cholera comprises the probe having the nucleotide sequence selected from SEQ ID NO:1 to SEQ ID NO:22.

Abstract translation: 提供一种用于检测霍乱弧菌霍乱的基因扩增试剂盒，通过分析霍乱弧菌某些基因的基因序列，准确，简单地检测霍乱弧菌诱发霍乱。 用于检测霍乱弧菌弧菌霍乱的基因扩增试剂盒包括由SEQ ID NO：1和SEQ ID NO：2组成的引物对，由SEQ ID NO：3和SEQ ID NO：4组成的引物对，引物对由 SEQ ID NO：9和SEQ ID NO：10的引物对，由SEQ ID NO：11和SEQ ID NO：12组成的引物对，由SEQ ID NO：13和SEQ ID NO：14组成的引物对，引物对 由SEQ ID NO：15和SEQ ID NO：16组成，由SEQ ID NO：17和SEQ ID NO：18组成的引物对，由SEQ ID NO：19和SEQ ID NO：20组成的引物对，引物 由SEQ ID NO：21和SEQ ID NO：22组成的对，或其组合。 用于检测霍乱弧菌弧菌霍乱的微阵列包括具有选自SEQ ID NO：1至SEQ ID NO：22的核苷酸序列的探针。

公开(公告)号：KR1020080078777A

公开(公告)日：2008-08-28

申请号：KR1020070112892

申请日：2007-11-06

Applicant: 서강대학교산학협력단 ,  국민대학교산학협력단

Inventor： 이정국 ,  이해진 ,  김의진 ,  성문희

IPC: C12Q1/18

CPC classification number: C12Q1/025 ,  G01N2333/90283

Abstract: A method for screening an antioxidant using mutant bacteria and chlorophyllide is provided to screen a large amount of the antioxidant easily by monitoring the growth state of the mutant bacteria and be useful for selective screening of an amphiphilic antioxidant, thereby applying the effective antioxidant with little toxicity as an additive of various foods and a cosmetic product. A method for screening an antioxidant comprises the steps of: (a) culturing mutant bacteria, which is transformed by including mutation in a DNA sequence encoding superoxide dismutase, thereby inhibiting the activity of the superoxide dismutase and expressing chlorophyllide reductase capable of inducing generation of superoxide anion in the presence of oxygen, in a plate; (b) locating a plurality of filter discs where an antioxidant candidate material to be screened and chlorophyllide are added on the plate; and (c) selecting the filter disc where the growth of the mutant bacteria is observed, wherein the bacteria to be mutated is Rhodobacter sphaeroides, Rhodospirillum rubrum, Rhodopseudomonas palustris, Allochromatium vinosum, Chlorobium tepidum or Chloroflexus aurantiacus. Further, an adding weight of chlorophyllide is 0.1 to 100 mM.

Abstract translation: 提供使用突变型细菌和叶绿素来筛选抗氧化剂的方法，通过监测突变细菌的生长状态容易地筛选大量的抗氧化剂，并且可用于选择性筛选两亲性抗氧化剂，从而将有效的抗氧化剂施用少量毒性 作为各种食品和化妆品的添加剂。 筛选抗氧化剂的方法包括以下步骤：（a）通过在编码超氧化物歧化酶的DNA序列中包含突变而转化突变细菌，从而抑制超氧化物歧化酶的活性并表达能诱导产生超氧化物的叶绿素还原酶 阴离子在氧的存在下，在板中; （b）定位多个滤盘，其中待筛选的抗氧化剂候选材料和叶绿素被添加到板上; （c）选择观察到突变细菌的生长的滤膜，其中待突变的细菌是球形红细菌，红色红孢菌，红假罗毕假单胞菌，紫苏胶，氧化铁或绿脓杆菌。 此外，叶绿素的添加重量为0.1〜100mM。