Abstract:
본 발명은 목적 유전자의 발현 억제 특이성이 증가되고 오프-타겟 효과가 감소된 siRNA 분자로서, 상기 siRNA 분자는 센스 및 안티센스 가닥으로 이루어져 있으며, 상기 두 가닥 중 하나의 가닥은 1 내지 3개의 뉴클레오티드의 DNA로 구성된 3'-오버행을 가지며, 나머지 하나의 가닥은 1 내지 3개의 뉴클레오티드의 RNA로 구성된 3'-오버행을 가지는 것을 특징으로 하는, 3'-오버행이 비대칭적인 구조를 갖는 siRNA 분자에 관한 것이다.
Abstract:
The present invention provides a method for regulating DRM (domain rearranged methyltransferase) protein activity in a plant comprising: base sequences represented by sequence number 1 or 2; a microRNA-396 molecule which consists of base sequences complementary to the above base sequences and which is isolated from Capsicum annuum; a vector containing the microRNA-396; and a step for regulating the expression of the microRNA-396 molecule by transfecting a plant cell with the recombinant vector. On the basis of a method for controlling all phenomena related to the florescence, seed development, and epigenetic phenomenon of the plant based on the fact that the aspect of regulating the DRM methyltransferase, which is highly involved in plant epigenetics, by the microRNA-396 from the Capsicum annuum is conserved in the Solanaceae including tomatoes revealed by the present invention, the DRM methyltransferase can be applied to agricultural biotechnology for improving traits of food crops, forage crops, floriculture crops, horticultural crops, and energy crops and be available for various medical applications like differentiation of cancer cells and neural cells, which are mainly involbed in epigenetic information, thereby being able to be very useful in many different fields.