公开(公告)号：KR101363412B1

公开(公告)日：2014-03-19

申请号：KR1020120111460

申请日：2012-10-08

Applicant: 서울대학교산학협력단

Inventor： 신찬석 ,  박준현 ,  황동규

IPC: A01H5/00 ,  C12N15/113 ,  C12N15/82

CPC classification number: C12N15/8218 ,  C07K14/415 ,  C12N9/1007 ,  C12N15/113 ,  C12N2310/141 ,  C12Y201/01037

Abstract: The present invention provides a method for regulating DRM (domain rearranged methyltransferase) protein activity in a plant comprising: base sequences represented by sequence number 1 or 2; a microRNA-396 molecule which consists of base sequences complementary to the above base sequences and which is isolated from Capsicum annuum; a vector containing the microRNA-396; and a step for regulating the expression of the microRNA-396 molecule by transfecting a plant cell with the recombinant vector. On the basis of a method for controlling all phenomena related to the florescence, seed development, and epigenetic phenomenon of the plant based on the fact that the aspect of regulating the DRM methyltransferase, which is highly involved in plant epigenetics, by the microRNA-396 from the Capsicum annuum is conserved in the Solanaceae including tomatoes revealed by the present invention, the DRM methyltransferase can be applied to agricultural biotechnology for improving traits of food crops, forage crops, floriculture crops, horticultural crops, and energy crops and be available for various medical applications like differentiation of cancer cells and neural cells, which are mainly involbed in epigenetic information, thereby being able to be very useful in many different fields.

Abstract translation: 本发明提供了一种用于调控植物中DRM（域重排甲基转移酶）蛋白活性的方法，包括：由序列号1或2表示的碱基序列; 由与上述碱基序列互补的碱基序列组成并从辣椒分离的微小RNA-396分子; 含有microRNA-396的载体; 以及通过用重组载体转染植物细胞来调节微小RNA-396分子的表达的步骤。 基于通过微小RNA-396调控高度参与植物表观遗传学的DRM甲基转移酶的方面，控制与植物的荧光，种子发育和表观遗传现象相关的所有现象的方法的基础上， 辣椒科中包括本发明披露的番茄科，其保藏的DRM甲基转移酶可用于农业生物技术，用于改善粮食作物，牧草作物，花卉作物，园艺作物和能源作物的性状，并可用于各种 医学应用如癌细胞和神经细胞的分化，其主要在表观遗传信息中被牵制，从而能够在许多不同领域中非常有用。