公开(公告)号：KR101164726B1

公开(公告)日：2012-07-12

申请号：KR1020090123711

申请日：2009-12-14

Applicant: 서울대학교산학협력단

Inventor： 이인원 ,  이정관 ,  박애란 ,  이승훈 ,  손호경

IPC: C12N15/63 ,  C12N1/21 ,  C12N15/82 ,  G01N33/02

CPC classification number: C12N15/80 ,  C12N15/8238 ,  C12N15/8239

Abstract: The present invention relates to a zearalenone-inducible promoter originated from Gibberella zeae, recombinant vectors comprising the promoters, methods of producing proteins and detecting plants contaminated with zearalenone. The promoter of the present invention can be used for studying essential genes in fungus, characterizing the mechanism of self/nonself recognition in G. zeae, engineering of plant systems to generate plants that can recognize a G. zeae attack and detecting ZEA contamination as a biosensor.

公开(公告)号：KR1020110067211A

公开(公告)日：2011-06-22

申请号：KR1020090123711

申请日：2009-12-14

Applicant: 서울대학교산학협력단

Inventor： 이인원 ,  이정관 ,  박애란 ,  이승훈 ,  손호경

IPC: C12N15/63 ,  C12N1/21 ,  C12N15/82 ,  G01N33/02

CPC classification number: C12N15/80 ,  C12N15/8238 ,  C12N15/8239

Abstract: PURPOSE: A zearalenone-inducible protein expression vector containing zearalenone-inducible promoter is provided to overexpress target genes and to detect zearalenone-polluted crops. CONSTITUTION: A recombinant vector has a base sequence of sequence number 1 and contains zearalenone-inducible promoter. The recombinant vector additionally contains a gene sequence encoding a target protein which is operatively linked to the inducible promoter. The target protein is a reporter protein. A method for preparing the proteins comprises: a step of culturing a recombinant microorganism in a medium containing zearalenone; a step of expressing the target gene; and a step of isolating proteins.

Abstract translation: 目的：提供含有玉米赤霉烯酮诱导型启动子的玉米赤霉烯酮诱导蛋白表达载体，以过表达目标基因并检测玉米赤霉烯酮污染的作物。 构成：重组载体具有序列号1的碱基序列并含有玉米赤霉烯酮可诱导启动子。 重组载体另外含有编码与可诱导启动子有效连接的靶蛋白的基因序列。 靶蛋白是报道蛋白。 制备蛋白质的方法包括：在含有玉米赤霉烯酮的培养基中培养重组微生物的步骤; 表达靶基因的步骤; 和分离蛋白质的步骤。