公开(公告)号：KR1020120047889A

公开(公告)日：2012-05-14

申请号：KR1020120044800

申请日：2012-04-27

Applicant: 서울대학교산학협력단

Inventor： 제연호 ,  최재영 ,  노종열 ,  김양수 ,  진병래 ,  우수동

IPC: C12N15/866 ,  C12N7/01 ,  C12N7/02

Abstract: PURPOSE: A baculovirus expression vector system using a recombinant virus bEasyBac is provided to enhance separation efficiency of the recombinant virus and to enable high-throughput of the recombinant virus. CONSTITUTION: A baculovirus expression vector system simultaneously produces a large amount of foreign gene-introduced recombinant virus and is bEasyBac(deposit number:KCTC 11499BP). The foreign gene is EGFP gene and is inserted in to a transfer vector pDualBac(5,964 bp). The expression system has Kanamycin resistant gene and mini F replicon. The expression system contains a lethal gene isolated from Bacillus amyloquefaciens and ORF 3005 promoter.

Abstract translation: 目的：提供使用重组病毒bEasyBac的杆状病毒表达载体系统，以提高重组病毒的分离效率，并使重组病毒能够高通量。 构成：杆状病毒表达载体系统同时产生大量引入外源基因的重组病毒，是bEasyBac（保藏号：KCTC 11499BP）。 外源基因是EGFP基因，插入转移载体pDualBac（5,964bp）。 表达系统具有卡那霉素抗性基因和迷你F复制子。 表达系统含有从解淀粉芽孢杆菌和ORF 3005启动子分离的致死基因。

公开(公告)号：KR1020100120515A

公开(公告)日：2010-11-16

申请号：KR1020090039373

申请日：2009-05-06

Applicant: 서울대학교산학협력단

Inventor： 제연호 ,  최재영 ,  노종열 ,  김양수 ,  진병래 ,  우수동

IPC: C12N15/866 ,  C12N7/01

Abstract: PURPOSE: A recombinant virus bEasyBac is provided to enhance isolation efficiency of recombinant virus and to produce a large amount of recombinant virus. CONSTITUTION: A baculovirus expression vector system is able to produce a large amount of recombinant virus in which various foreign genes are introduced. The baculovirus expression system is bEasyBac(deposit number : KCTC 11499BP) which is not able to proliferate in an insect cell line. A method for preparing the recomnbinant virus bEasyBac comprises: a step of preparing recombinant virus vApLaZ by co-transfection of DNA of pBac8-LacZ with bApGOZA DNA in sf9; a step of performing co-tranfection of plasmid pTen-MF DN to sf9 to obtain homologous recombinant virus bTenLacZ; a step of inducing transposition with plasmid pDPNR-3005 ProBarnase DNA; and a step of performing eletroporation to E.coli DH10B.

Abstract translation: 目的：提供重组病毒bEasyBac，以提高重组病毒的分离效率，并产生大量重组病毒。 构成：杆状病毒表达载体系统能够产生大量引入各种外来基因的重组病毒。 杆状病毒表达系统是在昆虫细胞系中不能增殖的bEasyBac（保藏号：KCTC 11499BP）。 制备重组病毒bEasyBac的方法包括：通过在sf9中共转染pBac8-LacZ的DNA与bApGOZA DNA来制备重组病毒vApLaZ的步骤; 将质粒pTen-MF DN共转染至sf9以获得同源重组病毒bTenLacZ的步骤; 诱导质粒pDPNR-3005 ProBarnase DNA转位的步骤; 以及对大肠杆菌DH10B进行电穿孔的步骤。

公开(公告)号：KR1020100049998A

公开(公告)日：2010-05-13

申请号：KR1020080109071

申请日：2008-11-04

Applicant: 충북대학교 산학협력단 ,  동아대학교 산학협력단 ,  서울대학교산학협력단 ,  주식회사 중앙백신연구소 ,  주식회사 팜한농

Inventor： 우수동 ,  구현나 ,  진병래 ,  이광식 ,  제연호 ,  노종열 ,  최재영 ,  윤인중 ,  유성식 ,  김재수 ,  김영인

IPC: C12N15/41 ,  C12N15/09 ,  C07K14/005

Abstract: PURPOSE: A novel porcine reproductive and respiratory syndrome virus(PRRSV) is provided to research of replication, transcription, and translation mechanism of PRRSV and develop diagnosis reagent and apparatus. CONSTITUTION: A DNA fragment encoding structure protein of porcine reproductive respiratory syndrome virus(PRRSV) is denoted by sequence number 1. The DNA fragment is denoted by sequence number 2, 3, 4, 5, 6, or 7. A vaccine composition for introducing antibody to PRRSV contains a polypeptide encoding with DNA fragment sequence.

Abstract translation: 目的：提供一种新型猪繁殖与呼吸综合征病毒（PRRSV），用于研究PRRSV的复制，转录和翻译机制，开发诊断试剂和仪器。 构成：编码猪繁殖呼吸综合征病毒（PRRSV）结构蛋白的DNA片段由序列号1表示.DNA片段由序列号2,3,4,5,6或7表示。用于引入的疫苗组合物 对PRRSV的抗体含有编码DNA片段序列的多肽。

公开(公告)号：KR101223577B1

公开(公告)日：2013-01-17

申请号：KR1020090039373

申请日：2009-05-06

Applicant: 서울대학교산학협력단

Inventor： 제연호 ,  최재영 ,  노종열 ,  김양수 ,  진병래 ,  우수동

IPC: C12N15/866 ,  C12N7/01

Abstract: 본 발명은 베큘로바이러스 발현벡터계 (Baculovirus Expression Vector System)에 조기 발현 프로모터 (Promter), 치사유전자 (Lethal gene) 및 전이유전자 (Transposition gene)를 도입함으로써, 외래유전자를 발현하는 재조합바이러스 (recombinant virus)의 분리효율을 높이고 대량생산(high-throughput)이 가능하도록 제작한 재조합바이러스 비이지박 (bEasyBac)을 개시한다.
베큘로바이러스 (baculovirus), 트랜스퍼벡터 (transfer vector), 바네이즈 (Barnase), 트랜스포지션 (Transposition), 재조합바이러스 (recombinant virus).

Abstract translation: 在bekyul本发明的病毒表达载体系统（杆状病毒表达载体系统）早期表达启动子（Promter），致死基因（致死基因）和通过引入基因的（换位基因），重组病毒表达外源基因（重组病毒到过渡 （BEasyBac），其产生以增加重组病毒的分离效率并实现高通量。

公开(公告)号：KR101233081B1

公开(公告)日：2013-02-14

申请号：KR1020120044800

申请日：2012-04-27

Applicant: 서울대학교산학협력단

Inventor： 제연호 ,  최재영 ,  노종열 ,  김양수 ,  진병래 ,  우수동

IPC: C12N15/866 ,  C12N7/01 ,  C12N7/02

Abstract: 본 발명은 베큘로바이러스 발현벡터계 (Baculovirus Expression Vector System)에 조기 발현 프로모터 (Promter), 치사유전자 (Lethal gene) 및 전이유전자 (Transposition gene)를 도입함으로써, 외래유전자를 발현하는 재조합바이러스 (recombinant virus)의 분리효율을 높이고 대량생산(high-throughput)이 가능하도록 제작한 재조합바이러스 비이지박 (bEasyBac)을 개시한다.

公开(公告)号：KR1020100052254A

公开(公告)日：2010-05-19

申请号：KR1020080111192

申请日：2008-11-10

Applicant: 충북대학교 산학협력단 ,  동아대학교 산학협력단 ,  서울대학교산학협력단 ,  주식회사 중앙백신연구소 ,  주식회사 팜한농

Inventor： 우수동 ,  구현나 ,  진병래 ,  이광식 ,  제연호 ,  노종열 ,  최재영 ,  윤인중 ,  유성식 ,  김재수 ,  김영인

IPC: C12N15/34 ,  C12N15/09

Abstract: PURPOSE: A gene sequence encoding glycoprotein gB, gC, and gD of swine Aujeszky's disease virus(ADV) is provided to develop virus vaccine and diagnosis reagent and apparatus and to prevent miscarriage of pregnant pig. CONSTITUTION: A DNA fragment encoding glycoprotein gB of swine Aujeszky's disease virus(ADV) NYJ(KCTC 11132BP) has a base sequence of sequence number 1. A DNA fragment encoding glycoprotein gC of swine Aujeszky's disease virus(ADV) NYJ(KCTC 11132BP) has a sequence of sequence number 2. A DNA fragment encoding glycoprotein gD of swine Aujeszky's disease virus(ADV) NYJ(KCTC 11132BP) has a sequence of sequence number 3. A polypeptide encoded by the DNA fragment has amino acid sequences of sequence number 4(gB), 5(gC), and 6(gD).

Abstract translation: 目的：提供编码猪Aujeszky病病毒（ADV）的糖蛋白gB，gC和gD的基因序列，开发病毒疫苗和诊断试剂和仪器，防止怀孕猪流产。 构成：编码猪Aujeszky病病毒（ADV）NYJ（KCTC 11132BP）的糖蛋白gB的DNA片段具有序列号1的碱基序列。编码猪Aujeszky病病毒（ADV）NYJ（KCTC 11132BP）的糖蛋白gC的DNA片段具有 序列号为2的序列。编码猪Aujeszky病病毒（ADV）NYJ（KCTC 11132BP）的糖蛋白gD的DNA片段具有序列号3的序列。由该DNA片段编码的多肽具有序列号4的氨基酸序列（ gB），5（gC）和6（gD）。