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公开(公告)号：KR101233081B1
公开(公告)日：2013-02-14
申请号：KR1020120044800
申请日：2012-04-27
Applicant: 서울대학교산학협력단
IPC: C12N15/866 , C12N7/01 , C12N7/02
Abstract: 본 발명은 베큘로바이러스 발현벡터계 (Baculovirus Expression Vector System)에 조기 발현 프로모터 (Promter), 치사유전자 (Lethal gene) 및 전이유전자 (Transposition gene)를 도입함으로써, 외래유전자를 발현하는 재조합바이러스 (recombinant virus)의 분리효율을 높이고 대량생산(high-throughput)이 가능하도록 제작한 재조합바이러스 비이지박 (bEasyBac)을 개시한다.
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Patent Agency Ranking
公开(公告)号：KR101409381B1
公开(公告)日：2014-06-27
申请号：KR1020110116487
申请日：2011-11-09
Applicant: 서울대학교산학협력단
IPC: A61K31/352 , A23K1/17 , A23K1/18 , A61K35/74
CPC classification number: Y02A50/469 , Y02A50/471
Abstract: 본 발명은 패실로미세스 바리오티(
Paecilomyces
variotii )의 유기용매 추출물을 유효성분으로 포함하는 어류 병원성 미생물의 감염 예방 또는 치료용 조성물에 관한 것이다. 본 발명에 따른 어류 병원성 미생물의 감염 예방 또는 치료용 조성물을 어류에 경구투여 또는 수조투여 할 경우, 현재 사용되고 있는 항생제를 투여하지 않고서도 충분히 어병에 대한 면역력을 확보할 수 있으므로, 어류의 세균에 의한 감염을 효과적으로 방지할 수 있고, 항생제를 대체할 수 있는 효과가 있다.-
公开(公告)号：KR1020120047889A
公开(公告)日：2012-05-14
申请号：KR1020120044800
申请日：2012-04-27
Applicant: 서울대학교산학협력단
IPC: C12N15/866 , C12N7/01 , C12N7/02
Abstract: PURPOSE: A baculovirus expression vector system using a recombinant virus bEasyBac is provided to enhance separation efficiency of the recombinant virus and to enable high-throughput of the recombinant virus. CONSTITUTION: A baculovirus expression vector system simultaneously produces a large amount of foreign gene-introduced recombinant virus and is bEasyBac(deposit number:KCTC 11499BP). The foreign gene is EGFP gene and is inserted in to a transfer vector pDualBac(5,964 bp). The expression system has Kanamycin resistant gene and mini F replicon. The expression system contains a lethal gene isolated from Bacillus amyloquefaciens and ORF 3005 promoter.
Abstract translation: 目的：提供使用重组病毒bEasyBac的杆状病毒表达载体系统，以提高重组病毒的分离效率，并使重组病毒能够高通量。 构成：杆状病毒表达载体系统同时产生大量引入外源基因的重组病毒，是bEasyBac（保藏号：KCTC 11499BP）。 外源基因是EGFP基因，插入转移载体pDualBac（5,964bp）。 表达系统具有卡那霉素抗性基因和迷你F复制子。 表达系统含有从解淀粉芽孢杆菌和ORF 3005启动子分离的致死基因。
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公开(公告)号：KR1020100120515A
公开(公告)日：2010-11-16
申请号：KR1020090039373
申请日：2009-05-06
Applicant: 서울대학교산학협력단
IPC: C12N15/866 , C12N7/01
Abstract: PURPOSE: A recombinant virus bEasyBac is provided to enhance isolation efficiency of recombinant virus and to produce a large amount of recombinant virus. CONSTITUTION: A baculovirus expression vector system is able to produce a large amount of recombinant virus in which various foreign genes are introduced. The baculovirus expression system is bEasyBac(deposit number : KCTC 11499BP) which is not able to proliferate in an insect cell line. A method for preparing the recomnbinant virus bEasyBac comprises: a step of preparing recombinant virus vApLaZ by co-transfection of DNA of pBac8-LacZ with bApGOZA DNA in sf9; a step of performing co-tranfection of plasmid pTen-MF DN to sf9 to obtain homologous recombinant virus bTenLacZ; a step of inducing transposition with plasmid pDPNR-3005 ProBarnase DNA; and a step of performing eletroporation to E.coli DH10B.
Abstract translation: 目的：提供重组病毒bEasyBac，以提高重组病毒的分离效率，并产生大量重组病毒。 构成：杆状病毒表达载体系统能够产生大量引入各种外来基因的重组病毒。 杆状病毒表达系统是在昆虫细胞系中不能增殖的bEasyBac（保藏号：KCTC 11499BP）。 制备重组病毒bEasyBac的方法包括：通过在sf9中共转染pBac8-LacZ的DNA与bApGOZA DNA来制备重组病毒vApLaZ的步骤; 将质粒pTen-MF DN共转染至sf9以获得同源重组病毒bTenLacZ的步骤; 诱导质粒pDPNR-3005 ProBarnase DNA转位的步骤; 以及对大肠杆菌DH10B进行电穿孔的步骤。
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公开(公告)号：KR1020130051523A
公开(公告)日：2013-05-21
申请号：KR1020110116487
申请日：2011-11-09
Applicant: 서울대학교산학협력단
IPC: A61K31/352 , A23K1/17 , A23K1/18 , A61K35/74
CPC classification number: Y02A50/469 , Y02A50/471 , A61K31/352 , A61K35/74
Abstract: PURPOSE: A use of a Paecilomyces variotii extract for preventing or treating pathogenic bacterial infection in fish is provided to ensure immunity to fish diseases, and to prevent bacterial infection in fish. CONSTITUTION: A composition for preventing or treating pathogenic bacterial infection in fish contains an organic solvent extract of Paecilomyces variotii as an active ingredient. The organic solvent is selected from the group consisting of n-hexane, chloroform, ethyl acetate, and butanol. The organic solvent is ethyl acetate. The composition contains a compound denoted by chemical formula 1 as an active ingredient. A method for preventing or treating pathogenic bacterial infection in fish comprises a step of directly treating a water bath in which fish grows with the composition, or adsorbing the composition to a feed for growing fish in a concentration of 1.0-500ppm.
Abstract translation: 目的：提供拟青霉提取物用于预防或治疗鱼类病原菌感染的用途，以确保对鱼类疾病的免疫力，并防止鱼类细菌感染。 构成：用于预防或治疗鱼类病原菌感染的组合物含有拟青霉的有机溶剂提取物作为活性成分。 有机溶剂选自正己烷，氯仿，乙酸乙酯和丁醇。 有机溶剂为乙酸乙酯。 组合物含有化学式1表示的化合物作为有效成分。 用于预防或治疗鱼类病原菌感染的方法包括直接处理其中鱼类与组合物生长的水浴或将组合物吸附至浓度为1.0-500ppm的鱼饲料的步骤。
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公开(公告)号：KR101223577B1
公开(公告)日：2013-01-17
申请号：KR1020090039373
申请日：2009-05-06
Applicant: 서울대학교산학협력단
IPC: C12N15/866 , C12N7/01
Abstract: 본 발명은 베큘로바이러스 발현벡터계 (Baculovirus Expression Vector System)에 조기 발현 프로모터 (Promter), 치사유전자 (Lethal gene) 및 전이유전자 (Transposition gene)를 도입함으로써, 외래유전자를 발현하는 재조합바이러스 (recombinant virus)의 분리효율을 높이고 대량생산(high-throughput)이 가능하도록 제작한 재조합바이러스 비이지박 (bEasyBac)을 개시한다.
베큘로바이러스 (baculovirus), 트랜스퍼벡터 (transfer vector), 바네이즈 (Barnase), 트랜스포지션 (Transposition), 재조합바이러스 (recombinant virus).Abstract translation: 在bekyul本发明的病毒表达载体系统（杆状病毒表达载体系统）早期表达启动子（Promter），致死基因（致死基因）和通过引入基因的（换位基因），重组病毒表达外源基因（重组病毒到过渡 （BEasyBac），其产生以增加重组病毒的分离效率并实现高通量。
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