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公开(公告)号：KR1020120047889A
公开(公告)日：2012-05-14
申请号：KR1020120044800
申请日：2012-04-27
Applicant: 서울대학교산학협력단
IPC: C12N15/866 , C12N7/01 , C12N7/02
Abstract: PURPOSE: A baculovirus expression vector system using a recombinant virus bEasyBac is provided to enhance separation efficiency of the recombinant virus and to enable high-throughput of the recombinant virus. CONSTITUTION: A baculovirus expression vector system simultaneously produces a large amount of foreign gene-introduced recombinant virus and is bEasyBac(deposit number:KCTC 11499BP). The foreign gene is EGFP gene and is inserted in to a transfer vector pDualBac(5,964 bp). The expression system has Kanamycin resistant gene and mini F replicon. The expression system contains a lethal gene isolated from Bacillus amyloquefaciens and ORF 3005 promoter.
Abstract translation: 目的：提供使用重组病毒bEasyBac的杆状病毒表达载体系统，以提高重组病毒的分离效率，并使重组病毒能够高通量。 构成：杆状病毒表达载体系统同时产生大量引入外源基因的重组病毒，是bEasyBac（保藏号：KCTC 11499BP）。 外源基因是EGFP基因，插入转移载体pDualBac（5,964bp）。 表达系统具有卡那霉素抗性基因和迷你F复制子。 表达系统含有从解淀粉芽孢杆菌和ORF 3005启动子分离的致死基因。
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公开(公告)号：KR1020100120515A
公开(公告)日：2010-11-16
申请号：KR1020090039373
申请日：2009-05-06
Applicant: 서울대학교산학협력단
IPC: C12N15/866 , C12N7/01
Abstract: PURPOSE: A recombinant virus bEasyBac is provided to enhance isolation efficiency of recombinant virus and to produce a large amount of recombinant virus. CONSTITUTION: A baculovirus expression vector system is able to produce a large amount of recombinant virus in which various foreign genes are introduced. The baculovirus expression system is bEasyBac(deposit number : KCTC 11499BP) which is not able to proliferate in an insect cell line. A method for preparing the recomnbinant virus bEasyBac comprises: a step of preparing recombinant virus vApLaZ by co-transfection of DNA of pBac8-LacZ with bApGOZA DNA in sf9; a step of performing co-tranfection of plasmid pTen-MF DN to sf9 to obtain homologous recombinant virus bTenLacZ; a step of inducing transposition with plasmid pDPNR-3005 ProBarnase DNA; and a step of performing eletroporation to E.coli DH10B.
Abstract translation: 目的：提供重组病毒bEasyBac，以提高重组病毒的分离效率，并产生大量重组病毒。 构成：杆状病毒表达载体系统能够产生大量引入各种外来基因的重组病毒。 杆状病毒表达系统是在昆虫细胞系中不能增殖的bEasyBac（保藏号：KCTC 11499BP）。 制备重组病毒bEasyBac的方法包括：通过在sf9中共转染pBac8-LacZ的DNA与bApGOZA DNA来制备重组病毒vApLaZ的步骤; 将质粒pTen-MF DN共转染至sf9以获得同源重组病毒bTenLacZ的步骤; 诱导质粒pDPNR-3005 ProBarnase DNA转位的步骤; 以及对大肠杆菌DH10B进行电穿孔的步骤。
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公开(公告)号：KR101286313B1
公开(公告)日：2013-07-15
申请号：KR1020100127988
申请日：2010-12-15
Applicant: 서울대학교산학협력단
IPC: C12N15/52 , C12N15/866
Abstract: 본 발명은 재조합 바이러스 에이씨이지-알비에스디브이1에스피(AcEasy-RBSDV1SP) 및 이를 이용한 베큘로바이러스 발현벡터 시스템에 관한 것으로, 벼검은줄오갈병 바이러스(rice black-streaked dwarf virus) 감염 벼의 조기진단을 위한 항원 확보와 특이항체 생산을 위하여 벼검은줄오갈병 바이러스 발현 유전자인 알엔에이-의존 알엔에이 폴리머라제(RNA-dependent RNA polymerase) 유전자를 베큘로바이러스 발현벡터계 (Baculovirus Expression Vector System)를 이용하여 대량 발현하는 방법을 제공한다.
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公开(公告)号：KR1020110115858A
公开(公告)日：2011-10-24
申请号：KR1020100035421
申请日：2010-04-16
Applicant: 서울대학교산학협력단
Abstract: 본 발명은
cry1Ac 유전자 발현 벡터 pHT1K-1Ac로 형질전환시킨 재조합 바실러스 벨레첸시스 균주 및 이를 유효성분으로 함유하는 식물병원균과 곤충 해충을 동시에 저해할 수 있는 살충제 방제용 조성물에 관한 것으로, 본 발명에 따른 재조합 바실러스 벨레첸시스 균주는 식물병원성균에 대해 높은 항균 활성나타내는 동시에 곤충 해충에 대한 저해 활성 또한 우수하여 하나의 작물에서 유해 곤충 해충과 식물병원성균에 의한 질병을 동시에 저해하는데 이용할 수 있으며, 상기 균주의 배양액 및 수득된 세포 모두를 통합 작물 보호용으로 개별 생물학적 저해제로서 독립적으로 사용할 수있는 효과가 있다-
5.发明公开모기독성을 갖는 바실러스 투린지엔시스 아종 모기 균주 및 이를 함유한 모기 살충제 조성물 无效BACILLUS THURINGIENSIS SUBSP。 具有针对多晶硅的器件活性的MOGI菌株和含有其的多晶型LARVICIDE组合物
公开(公告)号：KR1020110085159A
公开(公告)日：2011-07-27
申请号：KR1020100004782
申请日：2010-01-19
Applicant: 서울대학교산학협력단
Abstract: PURPOSE: An insecticidal composition for eliminating mosquito, containing novel Bacillus thuringiensis subsp. mogi strain is provided to ensure excellent insecticidal ability to Anopheles sinensis and Culex pipiens pallens. CONSTITUTION: A Bacillus thuringiensis subsp. Mogi strain(deposit number: KCTC 11565BP) having larvicidal activity is isolated from soil or fallen leaves. A mosquito is Anopheles sinensis and Culex pipiens pallens. An insecticidal composition for eliminating mosquito contains the strain as an active ingredient.
Abstract translation: 目的：消除蚊子的杀虫组合物，含有新型苏云金芽孢杆菌亚种。 提供mogi菌株以确保对中华按蚊和库蚊（Pulex pipiens pallens）的优良杀虫能力。 构成：苏云金芽孢杆菌亚种 从土壤或落叶中分离具有杀幼体活性的莫吉菌株（保藏号：KCTC 11565BP）。 一只蚊子是中华按蚊和库蚊（Pule）。 用于消除蚊子的杀虫组合物含有作为活性成分的菌株。
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Patent Agency Ranking
公开(公告)号：KR1020100049998A
公开(公告)日：2010-05-13
申请号：KR1020080109071
申请日：2008-11-04
Applicant: 충북대학교 산학협력단 , 동아대학교 산학협력단 , 서울대학교산학협력단 , 주식회사 중앙백신연구소 , 주식회사 팜한농
IPC: C12N15/41 , C12N15/09 , C07K14/005
Abstract: PURPOSE: A novel porcine reproductive and respiratory syndrome virus(PRRSV) is provided to research of replication, transcription, and translation mechanism of PRRSV and develop diagnosis reagent and apparatus. CONSTITUTION: A DNA fragment encoding structure protein of porcine reproductive respiratory syndrome virus(PRRSV) is denoted by sequence number 1. The DNA fragment is denoted by sequence number 2, 3, 4, 5, 6, or 7. A vaccine composition for introducing antibody to PRRSV contains a polypeptide encoding with DNA fragment sequence.
Abstract translation: 目的：提供一种新型猪繁殖与呼吸综合征病毒（PRRSV），用于研究PRRSV的复制，转录和翻译机制，开发诊断试剂和仪器。 构成：编码猪繁殖呼吸综合征病毒（PRRSV）结构蛋白的DNA片段由序列号1表示.DNA片段由序列号2,3,4,5,6或7表示。用于引入的疫苗组合物 对PRRSV的抗体含有编码DNA片段序列的多肽。
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公开(公告)号：KR101223577B1
公开(公告)日：2013-01-17
申请号：KR1020090039373
申请日：2009-05-06
Applicant: 서울대학교산학협력단
IPC: C12N15/866 , C12N7/01
Abstract: 본 발명은 베큘로바이러스 발현벡터계 (Baculovirus Expression Vector System)에 조기 발현 프로모터 (Promter), 치사유전자 (Lethal gene) 및 전이유전자 (Transposition gene)를 도입함으로써, 외래유전자를 발현하는 재조합바이러스 (recombinant virus)의 분리효율을 높이고 대량생산(high-throughput)이 가능하도록 제작한 재조합바이러스 비이지박 (bEasyBac)을 개시한다.
베큘로바이러스 (baculovirus), 트랜스퍼벡터 (transfer vector), 바네이즈 (Barnase), 트랜스포지션 (Transposition), 재조합바이러스 (recombinant virus).Abstract translation: 在bekyul本发明的病毒表达载体系统（杆状病毒表达载体系统）早期表达启动子（Promter），致死基因（致死基因）和通过引入基因的（换位基因），重组病毒表达外源基因（重组病毒到过渡 （BEasyBac），其产生以增加重组病毒的分离效率并实现高通量。
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公开(公告)号：KR101233081B1
公开(公告)日：2013-02-14
申请号：KR1020120044800
申请日：2012-04-27
Applicant: 서울대학교산학협력단
IPC: C12N15/866 , C12N7/01 , C12N7/02
Abstract: 본 발명은 베큘로바이러스 발현벡터계 (Baculovirus Expression Vector System)에 조기 발현 프로모터 (Promter), 치사유전자 (Lethal gene) 및 전이유전자 (Transposition gene)를 도입함으로써, 외래유전자를 발현하는 재조합바이러스 (recombinant virus)의 분리효율을 높이고 대량생산(high-throughput)이 가능하도록 제작한 재조합바이러스 비이지박 (bEasyBac)을 개시한다.
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9.发明公开
公开(公告)号：KR1020120089879A
公开(公告)日：2012-08-16
申请号：KR1020100127988
申请日：2010-12-15
Applicant: 서울대학교산학협력단
IPC: C12N15/52 , C12N15/866
Abstract: PURPOSE: A recombinant virus AcEasy-RBSDV1SP and a baculovirus expression vector system using the same are provided to diagnose rice black streaked dwarf virus. CONSTITUTION: An RNA-dependent RNA polymerase has a sequence of sequence number 1, isolated from rice black streaked dwarf virus. A transition vector, pDualBac-RBSDV1SP, contains a gene encoding RNA-dependent RNA polymerase. A recombinant virus is prepared by in vitro transposition of the transition vector to bEasyBac(KCTC 11499BP). A Baculovirus expression system for producing a large amount of RNA-dependent RNA polymerase of rice black streaked dwarf virus is prepared by transfection of recombinant virus AcEasy-RBSDV1SP(KCTC 11804BP) to insect cell line.
Abstract translation: 目的：提供重组病毒AcEasy-RBSDV1SP和使用该病毒的杆状病毒表达载体系统来诊断米黑条纹矮化病毒。 构成：RNA依赖性RNA聚合酶具有从米黑条纹矮化病毒中分离的序列号1的序列。 转移载体pDualBac-RBSDV1SP包含编码RNA依赖性RNA聚合酶的基因。 通过将转移载体体外转座至bEasyBac（KCTC 11499BP）制备重组病毒。 通过将重组病毒AcEasy-RBSDV1SP（KCTC 11804BP）转染到昆虫细胞系来制备用于产生大量黑色条纹矮化病毒的RNA依赖性RNA聚合酶的杆状病毒表达系统。
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公开(公告)号：KR1020100052254A
公开(公告)日：2010-05-19
申请号：KR1020080111192
申请日：2008-11-10
Applicant: 충북대학교 산학협력단 , 동아대학교 산학협력단 , 서울대학교산학협력단 , 주식회사 중앙백신연구소 , 주식회사 팜한농
Abstract: PURPOSE: A gene sequence encoding glycoprotein gB, gC, and gD of swine Aujeszky's disease virus(ADV) is provided to develop virus vaccine and diagnosis reagent and apparatus and to prevent miscarriage of pregnant pig. CONSTITUTION: A DNA fragment encoding glycoprotein gB of swine Aujeszky's disease virus(ADV) NYJ(KCTC 11132BP) has a base sequence of sequence number 1. A DNA fragment encoding glycoprotein gC of swine Aujeszky's disease virus(ADV) NYJ(KCTC 11132BP) has a sequence of sequence number 2. A DNA fragment encoding glycoprotein gD of swine Aujeszky's disease virus(ADV) NYJ(KCTC 11132BP) has a sequence of sequence number 3. A polypeptide encoded by the DNA fragment has amino acid sequences of sequence number 4(gB), 5(gC), and 6(gD).
Abstract translation: 目的：提供编码猪Aujeszky病病毒（ADV）的糖蛋白gB，gC和gD的基因序列，开发病毒疫苗和诊断试剂和仪器，防止怀孕猪流产。 构成：编码猪Aujeszky病病毒（ADV）NYJ（KCTC 11132BP）的糖蛋白gB的DNA片段具有序列号1的碱基序列。编码猪Aujeszky病病毒（ADV）NYJ（KCTC 11132BP）的糖蛋白gC的DNA片段具有 序列号为2的序列。编码猪Aujeszky病病毒（ADV）NYJ（KCTC 11132BP）的糖蛋白gD的DNA片段具有序列号3的序列。由该DNA片段编码的多肽具有序列号4的氨基酸序列（ gB），5（gC）和6（gD）。
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