公开(公告)号：KR101303990B1

公开(公告)日：2013-10-15

申请号：KR1020120035330

申请日：2012-04-05

Applicant: 성균관대학교산학협력단

Inventor： 권석태 ,  편혜우 ,  김인혜 ,  조성숙

IPC: C12N9/12 ,  C12N15/54 ,  C12N15/70 ,  C12N1/21

Abstract: PURPOSE: Thermococcus pacificus DNA polymerase variant is provided to be used in a various way such as gene diagnosis and PCR reaction thanks to being high in DNA amplification and amplification speed during PCR. CONSTITUTION: DNA polymerase comprises amino acid sequences chosen from a group comprised of sequence number 9, 10 and 11. A recombinant vector comprises amino acid sequence coding the amino acid sequences above. A host cell is transformed into the recombinant vector. The method of manufacturing DNA polymerase comprises the following steps. A recombinant vector which comprises base sequence s coding the amino acid is manufactured. The recombinant vector is transformed into a host cell. The transformed cell is cultivated. Protein from the transformed cell is collected.

Abstract translation: 目的：由于DNA扩增和PCR扩增速度高，提供了以多种方式进行基因诊断和PCR反应的太平洋热带球菌DNA聚合酶变体。 构成：DNA聚合酶包含选自序列号9,10和11的氨基酸序列。重组载体包含编码上述氨基酸序列的氨基酸序列。 将宿主细胞转化入重组载体。 制备DNA聚合酶的方法包括以下步骤。 制备包含编码氨基酸的碱基序列的重组载体。 将重组载体转化到宿主细胞中。 转化的细胞被培养。 收集来自转化细胞的蛋白质。