公开(公告)号：KR100991563B1

公开(公告)日：2010-11-04

申请号：KR1020080063027

申请日：2008-06-30

Applicant: 재단법인서울대학교산학협력재단

Inventor： 현진호 ,  정재연

IPC: G01N21/17 ,  G01N21/55 ,  G01N21/41 ,  G01N21/00

Abstract: 표면 플라즈몬 공명 센서칩, 그 제조 방법, 표면 플라즈몬 공명 센서 시스템 및 그를 이용한 분석 대상 물질 검출 방법이 개시된다. 표면 플라즈몬 공명 센서칩은 기판; 기판 상에 형성된 금속 박막; 및 금속 박막 상에 형성된 제1 유전체층을 포함한다.

公开(公告)号：KR100876657B1

公开(公告)日：2009-01-07

申请号：KR1020070103729

申请日：2007-10-15

Applicant: 한국기계연구원 ,  재단법인서울대학교산학협력재단

Inventor： 박상진 ,  현진호 ,  이종환 ,  김옥진 ,  김덕종 ,  고득용

IPC: G01N33/48 ,  G01N33/96

Abstract: A method for preparing a surface-modified microbead, a method for preparing a microbead array chip, and a method for checking the disease specific antigen by using the microbead array chip are provided to develop the smart system using elastin-like polypeptide (ELP). A method for preparing a surface-modified microbead comprises the steps of synthesizing the DNA base sequence by PCR based on the [(VGVPG)14(KGVPG)] trimer amino acid sequence to make an ELP expression recombinant vector, and transforming it to E. coli BLR(DE3) to make the biosynthesized ELP; and immobilizing the ELP on the surface of a microbead(40).

Abstract translation: 提供了制备表面改性微珠的方法，微珠阵列芯片的制备方法以及使用微珠阵列芯片检测疾病特异性抗原的方法，以开发使用弹性蛋白样多肽（ELP）的智能系统。 制备表面改性微珠的方法包括以下步骤：基于[（VGVPG）14（KGVPG）]三聚体氨基酸序列通过PCR合成DNA碱基序列以制备ELP表达重组载体，并将其转化为E. 大肠杆菌BLR（DE3）进行生物合成的ELP; 并将ELP固定在微珠（40）的表面上。

公开(公告)号：KR100772035B1

公开(公告)日：2007-10-31

申请号：KR1020060071295

申请日：2006-07-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 현진호

IPC: C12N7/08 ,  C12N7/00

Abstract: A method for preparing a virus plaque is provided to standardize the virus plaque forming method and obtain the virus plaque with excellent process efficiency and productivity. A method for preparing a virus plaque comprises the steps of: (a) preparing an engraved pattern of polydimethylsiloxane(PDMS) substrate(220); (b) applying a polymer material layer(305) to an embossed panel of the PDMS substrate with simultaneously culturing cells subject to be infected in the engraved pattern to prepare a cell array; and (c) infecting virus to the cultured cell and then culturing it to form a virus plaque. The PDMS substrate is prepared by the steps comprising: (i) applying a photoresist composition to a wafer; (ii) exposing, developing and drying the photoresist layer to form a channel pattern; (iii) coating PDMS on the wafer and drying it to solidify the PDMS; and (iv) isolating the solidified PDMS from the wafer.

Abstract translation: 提供一种制备病毒斑块的方法，以标准化病毒斑块形成方法，并以优异的工艺效率和生产率获得病毒斑块。 制备病毒斑块的方法包括以下步骤：（a）制备雕刻图案的聚二甲基硅氧烷（PDMS）底物（220）; （b）将聚合物材料层（305）施加到PDMS底物的压花面板上，同时以雕刻图案培养受感染的细胞以制备细胞阵列; 和（c）将病毒感染到培养的细胞中，然后培养以形成病毒斑块。 通过以下步骤制备PDMS衬底：（i）将光致抗蚀剂组合物施加到晶片上; （ii）曝光，显影和干燥光致抗蚀剂层以形成通道图案; （iii）在晶片上涂覆PDMS并干燥以固化PDMS; 和（iv）从晶片分离固化的PDMS。

公开(公告)号：KR1020070077948A

公开(公告)日：2007-07-30

申请号：KR1020060007876

申请日：2006-01-25

Applicant: 재단법인서울대학교산학협력재단

Inventor： 현진호

IPC: G01N33/53 ,  G01N33/58 ,  G01N33/48 ,  G01N33/00

Abstract: Provided is a method for preparing a bio-chip which is able to easily form a fine-structured pattern on a substrate and shows high process efficiency and economic efficiency. The method comprises the steps of: (a) forming an embossed pattern on the surface of a solidified polydimethylsiloxane(PDMS) to prepare a PDMS stamp; (b) preparing a subject substrate by washing and drying a substrate and then sequentially depositing Cr and Au on the surface of the substrate; (c) covering the embossed pattern of the PDMS stamp with a polymer material; (d) connecting the polymer material-covered PDMS stamp with the subject substrate so as to move the polymer material into the depositing side of the subject substrate corresponding to the embossed pattern, thereby forming a polymer pattern; and (e) separating the PDMS stamp from the subject substrate. The PDMS stamp is prepared by coating a photoresist composition on a wafer, exposing, developing and drying the photoresist layer to form a channel pattern on the wafer, coating and drying a liquid PDMS on the channel pattern-formed wafer to solidify the PDMS and then separating the solidified PDMS from the wafer.

Abstract translation: 本发明提供一种能够容易地在基板上形成精细结构的图案的生物芯片的制造方法，显示出高的工艺效率和经济性。 该方法包括以下步骤：（a）在固化的聚二甲基硅氧烷（PDMS）的表面上形成压花图案以制备PDMS印模; （b）通过洗涤和干燥衬底制备目标衬底，然后在衬底的表面上依次沉积Cr和Au; （c）用聚合物材料覆盖PDMS印模的压花图案; （d）将聚合物材料覆盖的PDMS印模与目标基板连接，以将聚合物材料移动到与压纹图案相对应的对象基板的沉积侧，从而形成聚合物图案; 和（e）将PDMS印模与目标基底分离。 通过将光致抗蚀剂组合物涂覆在晶片上，曝光，显影和干燥光致抗蚀剂层以在晶片上形成通道图案，涂覆和干燥通道图案形成的晶片上的液体PDMS以固化PDMS，然后再制备PDMS印模 将固化的PDMS与晶片分离。

公开(公告)号：KR1020100002960A

公开(公告)日：2010-01-07

申请号：KR1020080063027

申请日：2008-06-30

Applicant: 재단법인서울대학교산학협력재단

Inventor： 현진호 ,  정재연

IPC: G01N21/17 ,  G01N21/55 ,  G01N21/41 ,  G01N21/00

Abstract: PURPOSE: A surface plasmon resonance sensor chip, a manufacturing method thereof, a SPR system, and a detecting method of a material using the same are provided to detect a low molecular material or a tiny material by improving sensitivity to the material. CONSTITUTION: An SPR sensor chip(100) comprises a substrate(110), a metal film(120), a first dielectric layer(130), and a metallic nano-particle layer. The metal film is formed on the substrate. The first dielectric layer is formed on the metal film. The metallic nano-particle layer is formed on the first dielectric layer, and composed of metallic nano-particles.

Abstract translation: 目的：提供表面等离子体共振传感器芯片，其制造方法，SPR系统和使用其的材料的检测方法，以通过提高对材料的敏感性来检测低分子材料或微小材料。 构成：SPR传感器芯片（100）包括基板（110），金属膜（120），第一介电层（130）和金属纳米颗粒层。 金属膜形成在基板上。 第一电介质层形成在金属膜上。 金属纳米粒子层形成于第一电介质层上，由金属纳米粒子构成。

公开(公告)号：KR100772038B1

公开(公告)日：2007-10-31

申请号：KR1020060007876

申请日：2006-01-25

Applicant: 재단법인서울대학교산학협력재단

Inventor： 현진호

IPC: G01N33/53 ,  G01N33/58 ,  G01N33/48 ,  G01N33/00

Abstract: 본 발명의 일 특징에 따라 바이오 칩을 제조하기 위해서 우선 고형화된 폴리디메틸실록산(PolyDiMethylSiloxane, PDMS)의 표면에 양각 패턴을 형성하여 PDMS 스탬프를 제작한다. 대상 기판은 기판을 세척 및 건조하는 단계에 의하여 준비된다. 상기 제작된 PDMS 스탬프의 양각 패턴 상에는 액상의 고분자 물질이 도포된다. 상기 고분자 물질이 도포된 PDMS 스탬프는 상기 대상 기판과 접합됨으로써, 상기 고분자 물질이 상기 양각 패턴에 대응한 상기 대상 기판 면 상에 전이되고 상기 PDMS 스탬프를 상기 대상 기판으로부터 분리함으로써 고분자 패턴이 형성된다. 상기 고분자 패턴이 형성된 상기 기판 표면에 크롬 및 금을 순차적으로 증착함으로써 바이오 칩이 제조된다. 상기 제조 방법은 공정 효율 및 생산성이 매우 우수하다.