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1.发明授权
公开(公告)号：KR101581998B1
公开(公告)日：2016-01-04
申请号：KR1020130032561
申请日：2013-03-27
Applicant: 전남대학교산학협력단
IPC: C02F3/34
Abstract: 본발명에서는연안갯벌에서농화배양을통해엔도설판및 유기물분해능이매우우수한미생물 (균주명 EDB 2)를분리하여생리생화학적및 유전학적특성을분석한결과와 99%의높은상동성을보였다. 균주 EDB-2는호기성, 그람양성, 막대형이며, 핑크빛을띠고있다. 생장가능온도는 20℃~45℃, 최적온도는약 20℃~42℃로생장온도범위가비교적넓은것으로나타났다. Casein과 cellulose 가수분해능력은없지만 DNA, agar, starch 그리고 tween의분해능력이있는것으로나타나지질분해능이우수하여유기물로오염된수산폐수를정화하는데유용하게쓰일수 있을것이다.
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公开(公告)号：KR101477899B1
公开(公告)日：2015-01-08
申请号：KR1020130031960
申请日：2013-03-26
Applicant: 전남대학교산학협력단
Abstract: 본 발명은 연안 갯벌에서 유기물 분해능이 매우 우수한 것으로 알려진 갯지렁이(
Perinereis aibuhitensis )에 내생하고 있는 미생물을 MA 배지에서 생장하여 나타난 집락(colony)의 색깔과 모양에 따라 선별한 31개의 집락을 별도로 선별하여 순수배양한 후 16S rRNA 염기서열에 기초하여 동정한 결과,
Bacillus 속에 포함되는 균주를 분리하였다. 갯지렁이로부터 분리하여 선별한 두
Bacillus 균주 CBW4와 EBW4는 단백질, 탄수화물, 지질 분해능이 뛰어나고 염분에 내성을 갖고 있으므로 유기물로 오염된 양식장 폐수 환경을 정화하는데 유용하게 쓰일 수 있을 것이다.Abstract translation: 本发明涉及一种泻湖（泻湖）
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公开(公告)号：KR1020140117747A
公开(公告)日：2014-10-08
申请号：KR1020130032144
申请日：2013-03-26
Applicant: 전남대학교산학협력단
IPC: C02F3/34
Abstract: According to the present invention, microbes which live in Perinereis aibuhitensis in coastal mud flats have been grown in an MA culture medium and colonies have appeared, wherein the Perinereis aibuhitensis is known to have excellent degradability of organic matter. Thirty-one colonies have been separately selected according to the colors and shapes of the colonies which have appeared in the MA culture medium. Strains belonging to Bacillus genus have been isolated as a result of identification on the basis of 16S rRNA sequences after a pure culture process of the 31 colonies. Bacillus strains EBW4 and CBW4 isolated from the Perinereis aibuhitensis have excellent degradabilities of protein, carbohydrate and lipid and have resistance to salt. Accordingly, the EBW4 and CBW4 can be practically used to purify the wastewater environment of a farm contaminated with organic matter.
Abstract translation: 根据本发明，在沿海泥浆平面上生活在围Per中的微生物已经在MA培养基中生长，出现菌落，其中已知百日蓟马具有优异的有机物降解性。 根据出现在MA培养基中的菌落的颜色和形状分别选择了三十一个菌落。 在31个菌落的纯培养过程之后，基于16S rRNA序列的鉴定结果已经分离了属于芽孢杆菌属的菌株。 从百日蓟马分离的芽孢杆菌菌株EBW4和CBW4具有优异的蛋白质，碳水化合物和脂质的降解性，具有耐盐性。 因此，EBW4和CBW4实际上可用于净化有机物污染的农场的废水环境。
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公开(公告)号：KR1020140117732A
公开(公告)日：2014-10-08
申请号：KR1020130031984
申请日：2013-03-26
Applicant: 전남대학교산학협력단
CPC classification number: C02F3/341 , C02F2101/30 , C12N1/20
Abstract: The present invention separated a strain included in Bacillus sp. from the result of identification based on base sequence 16S rRNA after pure cultivating, by separately selecting thirty one colonies selected according to colors and shapes of colonies, generated by growing microorganism, arbuscular, in perinereis aibuhitensis known to have excellent ability of decomposing organic matters in mud flat along the shore, in MA medium. A strain CBW4 selected by being separated from perinereis aibuhitensis can be usefully used in purifying the environment filled with waste water in a farm contaminated due to the organic matters, by having salinity tolerance and excellent ability of decomposing protein, carbohydrate and lipid.
Abstract translation: 本发明分离芽孢杆菌属菌株中包含的菌株。 从纯培养后的基因序列16S rRNA的鉴定结果，通过分离选择根据生长的微生物生长的菌落的颜色和形状选择的三十一个菌落，已知具有优异的分解有机物质的能力的围is 沿岸平坦，MA中。 通过从围is中分离出来的菌株CBW4可以有效地用于净化由于有机物污染的农场中充满废水的环境，具有耐盐性和分解蛋白质，碳水化合物和脂质的优异能力。
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公开(公告)号：KR101476031B1
公开(公告)日：2014-12-24
申请号：KR1020130032144
申请日：2013-03-26
Applicant: 전남대학교산학협력단
IPC: C02F3/34
Abstract: 본발명은연안갯벌에서유기물분해능이매우우수한것으로알려진갯지렁이()에내생하고있는미생물을 MA 배지에서생장하여나타난집락(colony)의색깔과모양에따라선별한 31개의집락을별도로선별하여순수배양한후 16S rRNA 염기서열에기초하여동정한결과,속에포함되는균주를분리하였다. 갯지렁이로부터분리하여선별한균주 EBW4와 CBW4는단백질, 탄수화물, 지질분해능이뛰어나고염분에내성을갖고있으므로유기물로오염된양식장폐수환경을정화하는데유용하게쓰일수 있을것이다.
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Patent Agency Ranking
公开(公告)号：KR101455925B1
公开(公告)日：2014-11-03
申请号：KR1020130031984
申请日：2013-03-26
Applicant: 전남대학교산학협력단
Abstract: 본 발명은 연안 갯벌에서 유기물 분해능이 매우 우수한 것으로 알려진 갯지렁이(
Perinereis aibuhitensis )에 내생하고 있는 미생물을 MA 배지에서 생장하여 나타난 집락(colony)의 색깔과 모양에 따라 선별한 31개의 집락을 별도로 선별하여 순수배양한 후 16S rRNA 염기서열에 기초하여 동정한 결과,
Bacillus 속에 포함되는 균주를 분리하였다. 갯지렁이로부터 분리하여 선별한
Bacillus 균주 CBW4는 단백질, 탄수화물, 지질 분해능이 뛰어나고 염분에 내성을 갖고 있으므로 유기물로 오염된 양식장 폐수 환경을 정화하는데 유용하게 쓰일 수 있을 것이다.Abstract translation: 本发明涉及一种泻湖（泻湖）
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7.发明公开
公开(公告)号：KR1020140119856A
公开(公告)日：2014-10-13
申请号：KR1020130033007
申请日：2013-03-27
Applicant: 전남대학교산학협력단
Abstract: In the present invention, an enrichment culture is used to separate a microorganism from mud, whose strain name is EDB-2 and has an excellent ability to decompose endosulfan and organic materials, and the microorganism is analyzed for physiology properties and biochemistry properties. The result of the analysis shows that the microorganism has a 99% similarity to Rhodococcus pvridinivorans. The EDB-2 is aerobic, positive to gram, a bar type and pink. The growth temperature range of the EDB-2 is relatively large, the growable temperature being 20-45°C, and the optimal temperature thereof is 20-42°C, The EDB-2 cannot hydrolyze casein and cellulose but can decompose DNA, agar, starch and tween, which means the ability of the EDB-2 to decompose lipid is excellent. Accordingly the invention can be expected to be used for purifying marine waste water contaminated by organic materials.
Abstract translation: 在本发明中，使用富集培养物将微生物与菌株名称为EDB-2的微生物分离，并且具有优异的分解硫丹和有机物质的能力，分析微生物的生理特性和生物化学性质。 分析结果表明，微生物与红球菌（Rhodococcus pvridinivorans）具有99％的相似性。 EDB-2是有氧的，对于克，棒型和粉红色。 EDB-2的生长温度范围较大，可生长温度为20-45℃，最适温度为20-42℃。EDB-2不能水解酪蛋白和纤维素，但可分解DNA，琼脂 ，淀粉和吐温，这意味着EDB-2分解脂质的能力非常好。 因此，本发明可以预期用于净化被有机材料污染的海洋废水。
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公开(公告)号：KR1020140117730A
公开(公告)日：2014-10-08
申请号：KR1020130031960
申请日：2013-03-26
Applicant: 전남대학교산학협력단
CPC classification number: C12N1/20 , C02F3/341 , C02F2101/30
Abstract: The present invention separated a strain included in Bacillus sp. from the result of identification based on base sequence 16S rRNA after pure cultivating, by separately selecting thirty one colonies selected according to colors and shapes of colonies generated by growing microorganism, arbuscular, in perinereis aibuhitensis known to have excellent ability of decomposing organic matters in mud flats along the shore, in MA medium. Two strains CBW4 and EBW4 selected by being separated from perinereis aibuhitensis can be usefully used in purifying the environment filled with waste water in a farm contaminated due to the organic matters by having salinity tolerance and excellent ability of decomposing protein, carbohydrate and lipid.
Abstract translation: 本发明分离芽孢杆菌属菌株中包含的菌株。 根据基于纯培养后的碱基序列16S rRNA的鉴定结果，通过分离选择根据生长的微生物生长的菌落的颜色和形状选择的三十一个菌落，其中已知具有优异的分解有机物质在泥中的能力的围is 沿岸的单位，在MA中。 通过从围is being being中分离出来的两株CBW4和EBW4可以有效地用于净化由于有机物污染的农场中充满废水的环境，具有耐盐性和分解蛋白质，碳水化合物和脂质的优异能力。
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公开(公告)号：KR1020140119850A
公开(公告)日：2014-10-13
申请号：KR1020130032868
申请日：2013-03-27
Applicant: 전남대학교산학협력단
Abstract: According to the present invention, microbes which live in Perinereis aibuhitensis in coastal mud flats have been grown in a MA culture medium, thus leading to the appearance of colonies, wherein the Perinereis aibuhitensis is known to have excellent degradability of organic matter. Thirty-one colonies have been separately selected according to the colors and shapes of the colonies which have appeared in the MA culture medium. Strains belonging to Bacillus genus have been isolated as a result of identification on the basis of 16S rRNA sequences after a pure culture process of the 31 colonies. Two Bacillus strains, CBW4 and EBW4, isolated from the Perinereis aibuhitensis have excellent degradabilities of protein, carbohydrate and lipid, and have resistance to salt. Accordingly, the CBW4 and EBW4 can be practically used to purify the water environment of a biofloc culture system contaminated with organic matter.
Abstract translation: 根据本发明，在沿海泥浆平面上生活在围Per中的微生物已经在MA培养基中生长，从而导致菌落的出现，其中已知百日蓟马具有优异的有机物的降解性。 根据出现在MA培养基中的菌落的颜色和形状分别选择了三十一个菌落。 在31个菌落的纯培养过程之后，基于16S rRNA序列的鉴定结果已经分离了属于芽孢杆菌属的菌株。 从百日蔷薇中分离出的两种芽孢杆菌属菌株CBW4和EBW4具有优异的蛋白质，碳水化合物和脂质的降解性，具有耐盐性。 因此，CBW4和EBW4可以实际应用于净化有机物污染的生物絮凝培养体系的水环境。
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公开(公告)号：KR1020140119847A
公开(公告)日：2014-10-13
申请号：KR1020130032749
申请日：2013-03-27
Applicant: 전남대학교산학협력단
IPC: C02F3/34
Abstract: According to the present invention, microbes which live in Perinereis aibuhitensis in coastal mud flats have been grown in a MA culture medium, thus leading to the appearance of colonies, wherein the Perinereis aibuhitensis is knownto have excellent degradability of organic matter. Thirty-one colonies have been separately selected according to the colors and shapes of the colonies which have appeared in the MA culture medium. Strains belonging to Bacillus genus have been isolated as a result of identification on the basis of 16S rRNA sequences after a pure culture process of the 31 colonies. Two Bacillus strains, CBW4 and EBW4, isolated from the Perinereis aibuhitensis have excellent degradabilities of protein, carbohydrate and lipid, and have resistance to salt. Accordingly, the CBW4 and EBW4 can be practically used to purify the water environment of a biofloc culture system contaminated with organic matter.
Abstract translation: 根据本发明，在沿海泥浆平面上生活在围Per中的微生物已经在MA培养基中生长，从而导致菌落的出现，其中已知百日蓟马具有优异的有机物降解性。 根据出现在MA培养基中的菌落的颜色和形状分别选择了三十一个菌落。 在31个菌落的纯培养过程之后，基于16S rRNA序列的鉴定结果已经分离了属于芽孢杆菌属的菌株。 从百日蔷薇中分离出的两种芽孢杆菌属菌株CBW4和EBW4具有优异的蛋白质，碳水化合物和脂质的降解性，具有耐盐性。 因此，CBW4和EBW4可以实际应用于净化有机物污染的生物絮凝培养体系的水环境。
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