公开(公告)号：KR1020080085426A

公开(公告)日：2008-09-24

申请号：KR1020070026925

申请日：2007-03-20

Applicant: 충남대학교산학협력단

Inventor： 박창식 ,  김명철 ,  진동일 ,  이영주

IPC: C12N5/07 ,  C12N5/02 ,  C12N5/00

Abstract: A method for in vitro maturation of a porcine oocyte is provided to increase in vitro maturation rate of immature porcine oocyte, and reduce intrusion of multiple sperms, increase normal fertilization rate, and enhance blastocyst formation rate in the in vitro fertilization. A method for in vitro maturation of a porcine oocyte comprises the steps of: adding theca follicle into an in vitro maturation medium, which is prepared by adding 26.19 mM NaHCO3, 3.05 mM glucose, 0.91 mM sodium pyruvate, 75 mug/ml sodium penicillin G, 50 mug/ml streptomycin sulfate and 0.1% polyvinyl alcohol(PVA) in tissue culture medium(TCM) 199, in an amount of 1-6 theca follicle with the size of 2-4 mm per 2 ml of medium, and performing in vitro culture of the porcine oocyte in the in vitro maturation medium for 36-48 hours.

Abstract translation: 提供猪卵母细胞体外成熟的方法，以增加未成熟猪卵母细胞的体外成熟率，减少多次精子的侵入，增加正常受精率，增加体外受精中的囊胚形成率。 猪卵母细胞体外成熟的方法包括以下步骤：将卵泡加入到体外成熟培养基中，其通过加入26.19mM NaHCO 3，3.05mM葡萄糖，0.91mM丙酮酸钠，75ug / ml青霉素G ，组织培养基（TCM）199中的50μg/ ml硫酸链霉素硫酸盐和0.1％聚乙烯醇（PVA），其量为每2ml培养基尺寸为2-4mm的1-6个卵泡，并进行 体外培养猪卵母细胞体外成熟培养基36-48小时。

公开(公告)号：KR100807644B1

公开(公告)日：2008-02-28

申请号：KR1020070026707

申请日：2007-03-19

Applicant: 충남대학교산학협력단

Inventor： 박창식 ,  김명철 ,  진동일 ,  이영주

IPC: C12N5/16 ,  C12N5/00 ,  A01K67/00 ,  A01K67/02

CPC classification number: C12N5/0609 ,  A01K2227/108 ,  C12N2500/84 ,  C12N2501/11 ,  C12N2501/31 ,  C12N2517/10

Abstract: A method for producing piglet is provided to be able to increase the normal birth rate of cloned pig by co-transplantation of in vitro fertilization and somatic cell cloned fertilized eggs, significantly shorten the culturing period of the fertilized egg and improve the normal birth rate from a small number of ova. A method for producing piglet comprises the steps of: (a) culturing collected immature ova of slaughtered pig; (b) after preparing (i) a fertilized egg obtained by in vitro fertilization of the mature ova prepared by the step(a) and liquid semen, and (ii) a somatic cell transplanted fertilized egg obtained by transplanting a donor somatic cell into an enucleated prepared mature ova prepared by enucleating the mature ova of the step(a), respectively culturing (i) and (ii) for 16-36 hours; and (c) transplanting 150-250 fertilized eggs prepared by the in vitro fertilization and the somatic cell transplantation into one surrogate mother. Further, a culture ground of the immature ova contains 2-6 follicle films having 4-6 mm of size.

Abstract translation: 提供了一种生产小猪的方法，通过共同移植体外受精和体细胞克隆受精卵，能够提高克隆猪的正常出生率，大大缩短受精卵的培养期，提高正常出生率 少量的ova。 一种生产仔猪的方法包括以下步骤：（a）培养屠宰猪的未成熟卵; （b）制备（i）通过体外受精通过步骤（a）制备的成熟卵和液体精液获得的受精卵，和（ii）通过将供体体细胞移植到 通过摘除步骤（a）的成熟卵，分别培养（i）和（ii）16-36小时制备的去核制备的成熟卵; 和（c）将通过体外受精和体细胞移植制备的150-250个受精卵移植到一个代孕母亲中。 此外，未成熟卵的培养基含有2-6mm大小的2-6个卵泡膜。