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公开(公告)号：KR1020100109206A
公开(公告)日：2010-10-08
申请号：KR1020090027695
申请日：2009-03-31
Applicant: 충남대학교산학협력단
Abstract: 본 발명은 항산화 및 생식력 강화 활성을 갖는 음양곽(삼지구엽초; Epimedium koreanum Nakai) 건조분말 및 이의 추출물을 유효성분으로 함유하는 항산화제 또는 동물사료 첨가제 및 이를 포함하는 사료용 조성물에 관한 것으로, 본 발명의 음양곽 건조분말 및 이의 추출물은 항산화 효과를 가지고 있어 인간에게는 육질 개선, 이취 감소 및 각종 성인병 예방의 이익을, 가축에게는 소화력 증진, 사육 기간 단축, 사료비 절감 및 면역력 증대로 인한 각종 질병 예방의 이익을 주며, 또한, 음양곽의 유효성분인 이카린은 동물의 생식력 강화 및 자양강장의 기능을 부여하여 모체의 번식률을 높이는 효과를 발휘하므로 농가소득 증대에 기여할 수 있다.
음양곽, 이카린, 동물사료 첨가제, 사료, 항산화 활성, 생식력-
公开(公告)号：KR1020080085426A
公开(公告)日：2008-09-24
申请号：KR1020070026925
申请日：2007-03-20
Applicant: 충남대학교산학협력단
Abstract: A method for in vitro maturation of a porcine oocyte is provided to increase in vitro maturation rate of immature porcine oocyte, and reduce intrusion of multiple sperms, increase normal fertilization rate, and enhance blastocyst formation rate in the in vitro fertilization. A method for in vitro maturation of a porcine oocyte comprises the steps of: adding theca follicle into an in vitro maturation medium, which is prepared by adding 26.19 mM NaHCO3, 3.05 mM glucose, 0.91 mM sodium pyruvate, 75 mug/ml sodium penicillin G, 50 mug/ml streptomycin sulfate and 0.1% polyvinyl alcohol(PVA) in tissue culture medium(TCM) 199, in an amount of 1-6 theca follicle with the size of 2-4 mm per 2 ml of medium, and performing in vitro culture of the porcine oocyte in the in vitro maturation medium for 36-48 hours.
Abstract translation: 提供猪卵母细胞体外成熟的方法，以增加未成熟猪卵母细胞的体外成熟率，减少多次精子的侵入，增加正常受精率，增加体外受精中的囊胚形成率。 猪卵母细胞体外成熟的方法包括以下步骤：将卵泡加入到体外成熟培养基中，其通过加入26.19mM NaHCO 3，3.05mM葡萄糖，0.91mM丙酮酸钠，75ug / ml青霉素G ，组织培养基（TCM）199中的50μg/ ml硫酸链霉素硫酸盐和0.1％聚乙烯醇（PVA），其量为每2ml培养基尺寸为2-4mm的1-6个卵泡，并进行 体外培养猪卵母细胞体外成熟培养基36-48小时。
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公开(公告)号：KR100857592B1
公开(公告)日：2008-09-09
申请号：KR1020070054930
申请日：2007-06-05
Applicant: 충남대학교산학협력단
CPC classification number: G01N33/6851 , G01N33/582 , G01N27/44778
Abstract: A novel analytical method of protein by using CyDye is provided to compare and analyze two or more proteins simultaneously, analyze a trace amount of protein, increase reproducibility by removing preparation of prep gel for MALDI-TOF(matrix-assisted laser desorption ionization-time of fight), and enhance protein identification. A novel analytical method of protein by using CyDye comprises the steps of: (a) independently labeling a protein for analysis and a protein sample as control with different CyDye; (b) subjecting 50-100mug of each protein sample labeled in step (a) and 1-5 mg of the protein for analysis to 2-dimensional gel electrophoresis; (c) analyzing the difference between the protein for analysis and control protein by analyzing the electrophoresed gel through fluorescence analysis; and (d) isolating and identifying the proteins from the separated spot from the step (c) by using MALDI-TOF. Further, an automatic picker is used for the fluorescence analysis in the step (c).
Abstract translation: 提供了一种通过使用CyDye的新型蛋白质分析方法，同时比较和分析两种或更多种蛋白质，分析痕量的蛋白质，通过去除MALDI-TOF制备凝胶的制备增加了重现性（基质辅助激光解吸电离时间 战斗），并增强蛋白质鉴定。 通过使用CyDye的新型蛋白质分析方法包括以下步骤：（a）独立地将蛋白质标记用于分析，将蛋白质样品作为对照用不同的CyDye标记; （b）将50-100mug的步骤（a）中标记的每个蛋白质样品和1-5mg蛋白质进行二维凝胶电泳; （c）通过荧光分析分析电泳凝胶，分析蛋白质与蛋白质之间的差异; 和（d）通过使用MALDI-TOF从步骤（c）中分离的斑点分离和鉴定蛋白质。 此外，在步骤（c）中使用自动选择器进行荧光分析。
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公开(公告)号：KR1020080026949A
公开(公告)日：2008-03-26
申请号：KR1020060092212
申请日：2006-09-22
Applicant: 충남대학교산학협력단
Abstract: A method of breeding an animal by feeding Korean white pine(Pinus koraiensis) to the animal and functional meat products produced using the same are provided to improve animal immunity and meat quality and to reduce the off-flavor of meat. Leaves of Korean white pine is ground, mixed with feed and fed to animals to give meat raw material and its by-product or processed meat or its by-product. The mixing ratio of feed and Korean white pine leaf is 15 to 20:1 to 5. The meat raw material and its by-product contains pheasant meat, chicken meat, pork, horse meat, beef, lamb meat, duck meat, artificial meat, turkey meat, dehydrated eggs, eggs, frozen eggs, quail eggs, egg powder, duck eggs, duck liver. The processed meat or its by-product contains liver pate, liver paste, meat extract, meat jelly, pork cutlet, rennet, bacon, bouillon, broth, black pudding, beef steak, Charcuterie, sausage, cow's stomach, edible marrow, edible gelatin, dried meat slices, canned meat, bottled meat, baby-food meat, ham, hamburger meat, ground sausage, Korean sausage and sweet and sour pork(Tangsuyuk).
Abstract translation: 提供通过向动物喂养韩国白松（马齿松）和使用其生产的功能性肉制品来繁殖动物的方法，以提高动物免疫力和肉质，并减少肉的异味。 韩国白松叶粉碎，与饲料混合饲喂动物，给予肉类原料及其副产品或加工肉或副产品。 饲料和韩国白松叶的混合比例为15〜20：1〜5。肉类原料及其副产品含有雉鸡肉，鸡肉，猪肉，马肉，牛肉，羊肉，鸭肉，人造肉 ，火鸡肉，脱水鸡蛋，鸡蛋，冷冻鸡蛋，鹌鹑蛋，蛋粉，鸭蛋，鸭肝。 加工肉或其副产品含有肝汁，肝酱，肉提取物，肉冻，猪排，凝乳酶，培根，肉汤，肉汤，黑布丁，牛排，烧烤肉，香肠，牛胃，食用骨髓，食用明胶 ，干肉片，罐装肉，瓶装肉，婴儿食品肉，火腿，汉堡肉，地香肠，韩国香肠和酸甜猪肉（唐苏uk）。
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公开(公告)号：KR100710008B1
公开(公告)日：2007-04-23
申请号：KR1020060009923
申请日：2006-02-02
Applicant: 충남대학교산학협력단
Abstract: 본 발명은 체세포 복제수정란의 배발달율을 향상시키기 위한 것으로 (1) 공여핵 세포인 체세포를 streptolysin O를 포함하는 배지에서 체외배양하는 단계; 및 (2) 상기 체외배양된 체세포를 유전물질이 제거된 수핵난자에 이식하고 융합하여 체세포 복제수정란을 제조하는 단계; 로 이루어진 것을 특징으로 하는 체세포 복제수정란의 제조 방법에 관한 것이다.
본 발명에 의하면 SLO를 공여핵 세포에 처리한 후 수핵난자에 핵이식을 하였을 때 핵이식 후 융합율과 배반포까지의 배발달율이 유의성있게 증가하므로 체세포 복제 효율을 증진시킬 수 있다.
핵이식, 배발달율, 공여핵 세포, streptolysin O, 세포융합, 복제수정란, 체세포복제Abstract translation: 本发明包括体外培养的步骤（1）的体细胞的核供体细胞，以提高baldalyul克隆胚胎的倍在含培养基链球菌溶血素O; 并且（2）将体外培养的体细胞移植到已从其去除遗传物质的受体卵母细胞中并融合转基因胚胎以产生体细胞克隆胚胎; 本发明涉及生产体细胞克隆胚胎的方法。
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Patent Agency Ranking
公开(公告)号：KR100807644B1
公开(公告)日：2008-02-28
申请号：KR1020070026707
申请日：2007-03-19
Applicant: 충남대학교산학협력단
CPC classification number: C12N5/0609 , A01K2227/108 , C12N2500/84 , C12N2501/11 , C12N2501/31 , C12N2517/10
Abstract: A method for producing piglet is provided to be able to increase the normal birth rate of cloned pig by co-transplantation of in vitro fertilization and somatic cell cloned fertilized eggs, significantly shorten the culturing period of the fertilized egg and improve the normal birth rate from a small number of ova. A method for producing piglet comprises the steps of: (a) culturing collected immature ova of slaughtered pig; (b) after preparing (i) a fertilized egg obtained by in vitro fertilization of the mature ova prepared by the step(a) and liquid semen, and (ii) a somatic cell transplanted fertilized egg obtained by transplanting a donor somatic cell into an enucleated prepared mature ova prepared by enucleating the mature ova of the step(a), respectively culturing (i) and (ii) for 16-36 hours; and (c) transplanting 150-250 fertilized eggs prepared by the in vitro fertilization and the somatic cell transplantation into one surrogate mother. Further, a culture ground of the immature ova contains 2-6 follicle films having 4-6 mm of size.
Abstract translation: 提供了一种生产小猪的方法，通过共同移植体外受精和体细胞克隆受精卵，能够提高克隆猪的正常出生率，大大缩短受精卵的培养期，提高正常出生率 少量的ova。 一种生产仔猪的方法包括以下步骤：（a）培养屠宰猪的未成熟卵; （b）制备（i）通过体外受精通过步骤（a）制备的成熟卵和液体精液获得的受精卵，和（ii）通过将供体体细胞移植到 通过摘除步骤（a）的成熟卵，分别培养（i）和（ii）16-36小时制备的去核制备的成熟卵; 和（c）将通过体外受精和体细胞移植制备的150-250个受精卵移植到一个代孕母亲中。 此外，未成熟卵的培养基含有2-6mm大小的2-6个卵泡膜。
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公开(公告)号：KR1020090015007A
公开(公告)日：2009-02-11
申请号：KR1020080101219
申请日：2008-10-15
Applicant: 충남대학교산학협력단
IPC: C07D307/14 , C07D307/16
Abstract: A pig pheromonal urea-based compounds are provided to enhance the estrus induction efficiency of pig, facilitate the synthesis of compounds, stabilize the supply of the compounds, and reduce the compound production costs. The pig pheromonal urea-based compounds represented by the chemical formula(2) are useful for induction of estrus, wherein m and n are each independently 0 to 5; Z is O or S; R4 is 2-tetrahydrofuryl, 2-furanyl, phenyl, methoxy phenyl, toluyl, 3,5-dimethyl phenyl, 3-fluoro-6-methyl phenyl or pyridyl; R6 is methoxy phenyl, dimethoxyphenyl, dimethyl phenyl, ethylphenyl, 3-chloro-6-methoxy phenyl, 4-chloro-2-methyl phenyl, 3-chloro-6-methyl phenyl, fluorophenyl, chlorophenyl, 2,5-difluorophenyl or pyridyl; and R5 and R7 are each independently H or methyl group.
Abstract translation: 提供猪信息素尿素化合物以增强猪的发情诱导效率，促进化合物的合成，稳定化合物的供应，降低化合物的生产成本。 由化学式（2）表示的猪信息素脲基化合物可用于诱发发情，其中m和n各自独立地为0至5; Z是O或S; R4是2-四氢呋喃基，2-呋喃基，苯基，甲氧基苯基，甲苯酰基，3,5-二甲基苯基，3-氟-6-甲基苯基或吡啶基; R6是甲氧基苯基，二甲氧基苯基，二甲基苯基，乙基苯基，3-氯-6-甲氧基苯基，4-氯-2-甲基苯基，3-氯-6-甲基苯基，氟苯基，氯苯基，2,5-二氟苯基或吡啶基 ; 并且R 5和R 7各自独立地为H或甲基。
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公开(公告)号：KR100863572B1
公开(公告)日：2008-10-15
申请号：KR1020070060419
申请日：2007-06-20
Applicant: 충남대학교산학협력단
IPC: C07D307/12 , A61K31/341
Abstract: A novel pheromonal odorant is provided to be easily synthesized, thereby a composition comprising the same compound improving breeding efficiency through estrus synchronization of pig stably and economically and increasing the efficiency of pig farming management by decreasing labor force. A tetrahydrofuran based compound is represented by a formula(1). A composition for inducing estrus of pig or cow comprises the tetrahydrofuran based compound as an effective ingredient.
Abstract translation: 提供了一种易于合成的新型信息增感剂，由此提供了一种包含相同化合物的组合物，通过猪的发情同步稳定和经济地提高育种效率，并通过减少劳动力提高养猪管理的效率。 四氢呋喃类化合物由式（1）表示。 用于诱导猪或牛发情的组合物包括四氢呋喃类化合物作为有效成分。
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公开(公告)号：KR100844363B1
公开(公告)日：2008-07-07
申请号：KR1020070090073
申请日：2007-09-05
Applicant: 충남대학교산학협력단 , 솔젠트 (주)
IPC: A61K31/075 , A61P19/10
CPC classification number: A61K31/075
Abstract: A composition comprising D-pinitol is provided to show inhibitory effect on differentiation of osteoclast, thereby being very usefully used to prevent or treat metabolic bone diseases caused by hyperactivation of the osteoclast. A functional food composition for improving metabolic bone diseases comprises D-pinitol as an effective ingredient. A pharmaceutical composition for preventing or treating osteoporosis, Paget's disease, hypercalcemia, neoplastic destruction, cancer related bone resorption disease, osteolysis and rheumatoid arthritis comprises: a pharmaceutically effective amount of the D-pinitol; and a pharmaceutically acceptable carrier.
Abstract translation: 提供了包含D-壳糖醇的组合物以显示对破骨细胞分化的抑制作用，从而非常有用地用于预防或治疗由破骨细胞过度活化引起的代谢性骨疾病。 一种用于改善代谢性骨疾病的功能性食品组合物，其作为有效成分含有D-松香糖醇。 用于预防或治疗骨质疏松症，佩吉特氏病，高钙血症，肿瘤破坏，癌症相关骨吸收疾病，骨质溶解和类风湿性关节炎的药物组合物包括：药学有效量的D-松香糖醇; 和药学上可接受的载体。
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公开(公告)号：KR100987236B1
公开(公告)日：2010-10-12
申请号：KR1020090027695
申请日：2009-03-31
Applicant: 충남대학교산학협력단
Abstract: PURPOSE: A composition comprising dried powder and an extract of epimedium leaves, and a manufacturing method of an animal feed including thereof are provided to secure the generative power reinforcing activity of an antioxidant or the animal feed including the composition. CONSTITUTION: A manufacturing method of an animal feed including a composition comprising dried powder and an extract of epimedium leaves comprises the following steps: cutting the epimedium leaves; adding a mixed solution containing purified water and low class alcohol; and extracting for 1 hour~10 days in 15~30 deg C using an sonication extraction method; condensing and freeze-drying the extract; and mixing with the animal feed in a powder or granule state.
Abstract translation: 目的：提供一种包含干燥粉末和淫羊藿叶提取物的组合物及其包含动物饲料的制造方法，以确保抗氧化剂或包含该组合物的动物饲料的生成力增强活性。 构成：包含干粉和淫羊藿提取物的组合物的动物饲料的制造方法包括以下步骤：切割淫羊藿叶; 加入含有纯净水和低级酒精的混合溶液; 并使用超声处理提取法在15〜30℃下提取1〜10天; 冷凝干燥提取物; 并与粉末或颗粒状态的动物饲料混合。
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