公开(公告)号：KR101733818B1

公开(公告)日：2017-05-08

申请号：KR1020120122801

申请日：2012-11-01

Applicant: 충남대학교산학협력단

Inventor： 진동일 ,  장진옥

IPC: C12N5/02 ,  C12N5/075

Abstract: 본발명은포유동물의난모세포성숙화및 배아발달촉진용배지조성물및 이를이용한난모세포성숙화및 배아발달촉진방법에관한것으로서, 보다구체적으로는타우로우르소데옥시콜레이트(tauroursodeoxycholate)를포함하는포유동물난모세포성숙화및 배아발달촉진용배지와이를이용한포유동물난모세포성숙화및 배아발달촉진방법에관한것이다. 본발명의배지조성물및 이를이용한포유동물난모세포성숙화및 배아발달촉진방법에의하면, 포유동물의난모세포를대상으로효율적인난모세포의성숙화및 배아발달을촉진시킬수 있다.

Abstract translation: 本发明细胞我与哺乳动物卵母细胞成熟培养基组成，和用于促进和胚胎发育它涉及到成熟和促进胚胎发育的方法，并且更具体地euroneun tau蛋白含有乌尔的sode氧基胆哺乳动物卵母细胞（tauroursodeoxycholate） 一种促进成熟和胚胎发育的培养基，以及使用该培养基促进哺乳动物卵母细胞成熟和胚胎发育的方法。 根据本发明的哺乳动物卵母细胞成熟和使用本发明的培养基成分促进胚胎发育的方法和此，在哺乳动物中I I能有效地靶向细胞可以sikilsu促进成熟和所述的卵母细胞的胚胎发育。

公开(公告)号：KR102130897B1

公开(公告)日：2020-07-06

申请号：KR1020180121758

申请日：2018-10-12

Applicant: 충남대학교산학협력단

Inventor： 진동일 ,  이재은

IPC: C12Q1/6888 ,  G01N33/68

公开(公告)号：KR1020130000950A

公开(公告)日：2013-01-03

申请号：KR1020110061779

申请日：2011-06-24

Applicant: 충남대학교산학협력단

Inventor： 진동일 ,  한영훈 ,  김홍래

IPC: G01N33/53 ,  C12N15/11 ,  C12Q1/68

CPC classification number: C12Q1/6876 ,  C07K16/18 ,  C07K2317/34 ,  C12Q2600/124 ,  C12Q2600/158 ,  G01N33/6872 ,  G01N33/689 ,  G01N33/5302

Abstract: PURPOSE: A marker composition for diagnosing bovine pregnancy using pregnancy-specific proteins, and a composition for diagnosing pregnancy are provided to quickly and accurately determine pregnancy. CONSTITUTION: A composition for diagnosing bovine pregnancy contains a material which measures mRNA or protein level of alpha 1G of a T-type calcium channel. The alpha 1G protein has amino acid of sequence number 1. The material which measures the alpha 1G mRNA level is a primer or a probe. The material which measures the alpha 1G protein level is an antibody which specifically recognizes the alpha 1G protein. The antibody contains amino acid of sequence number 2. A kit for diagnosing bovine pregnancy contains the composition.

Abstract translation: 目的：使用妊娠特异性蛋白诊断牛怀孕的标记物组合物和用于诊断妊娠的组合物可快速准确地确定怀孕。 构成：用于诊断牛妊娠的组合物含有测量T型钙通道α1G的mRNA或蛋白质水平的物质。 α1G蛋白具有序列号1的氨基酸。测量α1GmRNA水平的物质是引物或探针。 测量α1G蛋白水平的物质是特异性识别α1G蛋白的抗体。 抗体含有序列号2的氨基酸。用于诊断牛妊娠的试剂盒包含组合物。

公开(公告)号：KR1020080085426A

公开(公告)日：2008-09-24

申请号：KR1020070026925

申请日：2007-03-20

Applicant: 충남대학교산학협력단

Inventor： 박창식 ,  김명철 ,  진동일 ,  이영주

IPC: C12N5/07 ,  C12N5/02 ,  C12N5/00

Abstract: A method for in vitro maturation of a porcine oocyte is provided to increase in vitro maturation rate of immature porcine oocyte, and reduce intrusion of multiple sperms, increase normal fertilization rate, and enhance blastocyst formation rate in the in vitro fertilization. A method for in vitro maturation of a porcine oocyte comprises the steps of: adding theca follicle into an in vitro maturation medium, which is prepared by adding 26.19 mM NaHCO3, 3.05 mM glucose, 0.91 mM sodium pyruvate, 75 mug/ml sodium penicillin G, 50 mug/ml streptomycin sulfate and 0.1% polyvinyl alcohol(PVA) in tissue culture medium(TCM) 199, in an amount of 1-6 theca follicle with the size of 2-4 mm per 2 ml of medium, and performing in vitro culture of the porcine oocyte in the in vitro maturation medium for 36-48 hours.

Abstract translation: 提供猪卵母细胞体外成熟的方法，以增加未成熟猪卵母细胞的体外成熟率，减少多次精子的侵入，增加正常受精率，增加体外受精中的囊胚形成率。 猪卵母细胞体外成熟的方法包括以下步骤：将卵泡加入到体外成熟培养基中，其通过加入26.19mM NaHCO 3，3.05mM葡萄糖，0.91mM丙酮酸钠，75ug / ml青霉素G ，组织培养基（TCM）199中的50μg/ ml硫酸链霉素硫酸盐和0.1％聚乙烯醇（PVA），其量为每2ml培养基尺寸为2-4mm的1-6个卵泡，并进行 体外培养猪卵母细胞体外成熟培养基36-48小时。

公开(公告)号：KR100857592B1

公开(公告)日：2008-09-09

申请号：KR1020070054930

申请日：2007-06-05

Applicant: 충남대학교산학협력단

Inventor： 진동일 ,  이재영 ,  김홍래 ,  박창식

IPC: G01N33/68 ,  G01N33/53 ,  G01N33/50 ,  G01N33/48

CPC classification number: G01N33/6851 ,  G01N33/582 ,  G01N27/44778

Abstract: A novel analytical method of protein by using CyDye is provided to compare and analyze two or more proteins simultaneously, analyze a trace amount of protein, increase reproducibility by removing preparation of prep gel for MALDI-TOF(matrix-assisted laser desorption ionization-time of fight), and enhance protein identification. A novel analytical method of protein by using CyDye comprises the steps of: (a) independently labeling a protein for analysis and a protein sample as control with different CyDye; (b) subjecting 50-100mug of each protein sample labeled in step (a) and 1-5 mg of the protein for analysis to 2-dimensional gel electrophoresis; (c) analyzing the difference between the protein for analysis and control protein by analyzing the electrophoresed gel through fluorescence analysis; and (d) isolating and identifying the proteins from the separated spot from the step (c) by using MALDI-TOF. Further, an automatic picker is used for the fluorescence analysis in the step (c).

Abstract translation: 提供了一种通过使用CyDye的新型蛋白质分析方法，同时比较和分析两种或更多种蛋白质，分析痕量的蛋白质，通过去除MALDI-TOF制备凝胶的制备增加了重现性（基质辅助激光解吸电离时间 战斗），并增强蛋白质鉴定。 通过使用CyDye的新型蛋白质分析方法包括以下步骤：（a）独立地将蛋白质标记用于分析，将蛋白质样品作为对照用不同的CyDye标记; （b）将50-100mug的步骤（a）中标记的每个蛋白质样品和1-5mg蛋白质进行二维凝胶电泳; （c）通过荧光分析分析电泳凝胶，分析蛋白质与蛋白质之间的差异; 和（d）通过使用MALDI-TOF从步骤（c）中分离的斑点分离和鉴定蛋白质。 此外，在步骤（c）中使用自动选择器进行荧光分析。

公开(公告)号：KR100710008B1

公开(公告)日：2007-04-23

申请号：KR1020060009923

申请日：2006-02-02

Applicant: 충남대학교산학협력단

Inventor： 진동일 ,  나루세겐지 ,  박창식

IPC: C12N5/07 ,  C12N5/071 ,  C12N5/02

Abstract: 본 발명은 체세포 복제수정란의 배발달율을 향상시키기 위한 것으로 (1) 공여핵 세포인 체세포를 streptolysin O를 포함하는 배지에서 체외배양하는 단계; 및 (2) 상기 체외배양된 체세포를 유전물질이 제거된 수핵난자에 이식하고 융합하여 체세포 복제수정란을 제조하는 단계; 로 이루어진 것을 특징으로 하는 체세포 복제수정란의 제조 방법에 관한 것이다.
본 발명에 의하면 SLO를 공여핵 세포에 처리한 후 수핵난자에 핵이식을 하였을 때 핵이식 후 융합율과 배반포까지의 배발달율이 유의성있게 증가하므로 체세포 복제 효율을 증진시킬 수 있다.
핵이식, 배발달율, 공여핵 세포, streptolysin O, 세포융합, 복제수정란, 체세포복제

Abstract translation: 本发明包括体外培养的步骤（1）的体细胞的核供体细胞，以提高baldalyul克隆胚胎的倍在含培养基链球菌溶血素O; 并且（2）将体外培养的体细胞移植到已从其去除遗传物质的受体卵母细胞中并融合转基因胚胎以产生体细胞克隆胚胎; 本发明涉及生产体细胞克隆胚胎的方法。

公开(公告)号：KR1020140055651A

公开(公告)日：2014-05-09

申请号：KR1020120122801

申请日：2012-11-01

Applicant: 충남대학교산학협력단

Inventor： 진동일 ,  장진옥

IPC: C12N5/02 ,  C12N5/075

Abstract: The present invention relates to a medium composition for oocytes maturation and embryo development promotion of mammals, and methods for oocytes maturation and embryo development promotion of mammals using the same. More specifically, the said invention relates to a medium for oocytes maturation and embryo development promotion of mammals comprising tauroursodeoxycholate, and methods for oocytes maturation and embryo development promotion of mammals using the same. According to the medium composition and the methods for oocytes maturation and embryo development promotion of mammals using the same of the present invention, effective oocytes maturation and embryo development can be promoted for oocytes of mammals.

Abstract translation: 本发明涉及用于哺乳动物的卵母细胞成熟和胚胎发育促进的培养基组合物，以及使用其的哺乳动物的卵母细胞成熟和胚胎发育促进的方法。 更具体地，本发明涉及包含牛磺脱氧胆酸的哺乳动物的卵母细胞成熟和胚胎发育促进的培养基，以及使用其的哺乳动物的卵母细胞成熟和胚胎发育促进的方法。 根据使用本发明的哺乳动物的培养基组成和卵母细胞成熟和胚胎发育促进方法，可以促进哺乳动物的卵母细胞的有效卵母细胞成熟和胚胎发育。

公开(公告)号：KR100807644B1

公开(公告)日：2008-02-28

申请号：KR1020070026707

申请日：2007-03-19

Applicant: 충남대학교산학협력단

Inventor： 박창식 ,  김명철 ,  진동일 ,  이영주

IPC: C12N5/16 ,  C12N5/00 ,  A01K67/00 ,  A01K67/02

CPC classification number: C12N5/0609 ,  A01K2227/108 ,  C12N2500/84 ,  C12N2501/11 ,  C12N2501/31 ,  C12N2517/10

Abstract: A method for producing piglet is provided to be able to increase the normal birth rate of cloned pig by co-transplantation of in vitro fertilization and somatic cell cloned fertilized eggs, significantly shorten the culturing period of the fertilized egg and improve the normal birth rate from a small number of ova. A method for producing piglet comprises the steps of: (a) culturing collected immature ova of slaughtered pig; (b) after preparing (i) a fertilized egg obtained by in vitro fertilization of the mature ova prepared by the step(a) and liquid semen, and (ii) a somatic cell transplanted fertilized egg obtained by transplanting a donor somatic cell into an enucleated prepared mature ova prepared by enucleating the mature ova of the step(a), respectively culturing (i) and (ii) for 16-36 hours; and (c) transplanting 150-250 fertilized eggs prepared by the in vitro fertilization and the somatic cell transplantation into one surrogate mother. Further, a culture ground of the immature ova contains 2-6 follicle films having 4-6 mm of size.

Abstract translation: 提供了一种生产小猪的方法，通过共同移植体外受精和体细胞克隆受精卵，能够提高克隆猪的正常出生率，大大缩短受精卵的培养期，提高正常出生率 少量的ova。 一种生产仔猪的方法包括以下步骤：（a）培养屠宰猪的未成熟卵; （b）制备（i）通过体外受精通过步骤（a）制备的成熟卵和液体精液获得的受精卵，和（ii）通过将供体体细胞移植到 通过摘除步骤（a）的成熟卵，分别培养（i）和（ii）16-36小时制备的去核制备的成熟卵; 和（c）将通过体外受精和体细胞移植制备的150-250个受精卵移植到一个代孕母亲中。 此外，未成熟卵的培养基含有2-6mm大小的2-6个卵泡膜。