公开(公告)号：KR1020130000950A

公开(公告)日：2013-01-03

申请号：KR1020110061779

申请日：2011-06-24

Applicant: 충남대학교산학협력단

Inventor： 진동일 ,  한영훈 ,  김홍래

IPC: G01N33/53 ,  C12N15/11 ,  C12Q1/68

CPC classification number: C12Q1/6876 ,  C07K16/18 ,  C07K2317/34 ,  C12Q2600/124 ,  C12Q2600/158 ,  G01N33/6872 ,  G01N33/689 ,  G01N33/5302

Abstract: PURPOSE: A marker composition for diagnosing bovine pregnancy using pregnancy-specific proteins, and a composition for diagnosing pregnancy are provided to quickly and accurately determine pregnancy. CONSTITUTION: A composition for diagnosing bovine pregnancy contains a material which measures mRNA or protein level of alpha 1G of a T-type calcium channel. The alpha 1G protein has amino acid of sequence number 1. The material which measures the alpha 1G mRNA level is a primer or a probe. The material which measures the alpha 1G protein level is an antibody which specifically recognizes the alpha 1G protein. The antibody contains amino acid of sequence number 2. A kit for diagnosing bovine pregnancy contains the composition.

Abstract translation: 目的：使用妊娠特异性蛋白诊断牛怀孕的标记物组合物和用于诊断妊娠的组合物可快速准确地确定怀孕。 构成：用于诊断牛妊娠的组合物含有测量T型钙通道α1G的mRNA或蛋白质水平的物质。 α1G蛋白具有序列号1的氨基酸。测量α1GmRNA水平的物质是引物或探针。 测量α1G蛋白水平的物质是特异性识别α1G蛋白的抗体。 抗体含有序列号2的氨基酸。用于诊断牛妊娠的试剂盒包含组合物。

公开(公告)号：KR100857592B1

公开(公告)日：2008-09-09

申请号：KR1020070054930

申请日：2007-06-05

Applicant: 충남대학교산학협력단

Inventor： 진동일 ,  이재영 ,  김홍래 ,  박창식

IPC: G01N33/68 ,  G01N33/53 ,  G01N33/50 ,  G01N33/48

CPC classification number: G01N33/6851 ,  G01N33/582 ,  G01N27/44778

Abstract: A novel analytical method of protein by using CyDye is provided to compare and analyze two or more proteins simultaneously, analyze a trace amount of protein, increase reproducibility by removing preparation of prep gel for MALDI-TOF(matrix-assisted laser desorption ionization-time of fight), and enhance protein identification. A novel analytical method of protein by using CyDye comprises the steps of: (a) independently labeling a protein for analysis and a protein sample as control with different CyDye; (b) subjecting 50-100mug of each protein sample labeled in step (a) and 1-5 mg of the protein for analysis to 2-dimensional gel electrophoresis; (c) analyzing the difference between the protein for analysis and control protein by analyzing the electrophoresed gel through fluorescence analysis; and (d) isolating and identifying the proteins from the separated spot from the step (c) by using MALDI-TOF. Further, an automatic picker is used for the fluorescence analysis in the step (c).

Abstract translation: 提供了一种通过使用CyDye的新型蛋白质分析方法，同时比较和分析两种或更多种蛋白质，分析痕量的蛋白质，通过去除MALDI-TOF制备凝胶的制备增加了重现性（基质辅助激光解吸电离时间 战斗），并增强蛋白质鉴定。 通过使用CyDye的新型蛋白质分析方法包括以下步骤：（a）独立地将蛋白质标记用于分析，将蛋白质样品作为对照用不同的CyDye标记; （b）将50-100mug的步骤（a）中标记的每个蛋白质样品和1-5mg蛋白质进行二维凝胶电泳; （c）通过荧光分析分析电泳凝胶，分析蛋白质与蛋白质之间的差异; 和（d）通过使用MALDI-TOF从步骤（c）中分离的斑点分离和鉴定蛋白质。 此外，在步骤（c）中使用自动选择器进行荧光分析。