公开(公告)号：KR102099164B1

公开(公告)日：2020-04-09

申请号：KR1020180172881

申请日：2018-12-28

Applicant: 충남대학교산학협력단

Inventor： 조병관 ,  이재영

IPC: G01N21/31 ,  G01N33/02 ,  G01N21/17

公开(公告)号：KR100857592B1

公开(公告)日：2008-09-09

申请号：KR1020070054930

申请日：2007-06-05

Applicant: 충남대학교산학협력단

Inventor： 진동일 ,  이재영 ,  김홍래 ,  박창식

IPC: G01N33/68 ,  G01N33/53 ,  G01N33/50 ,  G01N33/48

CPC classification number: G01N33/6851 ,  G01N33/582 ,  G01N27/44778

Abstract: A novel analytical method of protein by using CyDye is provided to compare and analyze two or more proteins simultaneously, analyze a trace amount of protein, increase reproducibility by removing preparation of prep gel for MALDI-TOF(matrix-assisted laser desorption ionization-time of fight), and enhance protein identification. A novel analytical method of protein by using CyDye comprises the steps of: (a) independently labeling a protein for analysis and a protein sample as control with different CyDye; (b) subjecting 50-100mug of each protein sample labeled in step (a) and 1-5 mg of the protein for analysis to 2-dimensional gel electrophoresis; (c) analyzing the difference between the protein for analysis and control protein by analyzing the electrophoresed gel through fluorescence analysis; and (d) isolating and identifying the proteins from the separated spot from the step (c) by using MALDI-TOF. Further, an automatic picker is used for the fluorescence analysis in the step (c).

Abstract translation: 提供了一种通过使用CyDye的新型蛋白质分析方法，同时比较和分析两种或更多种蛋白质，分析痕量的蛋白质，通过去除MALDI-TOF制备凝胶的制备增加了重现性（基质辅助激光解吸电离时间 战斗），并增强蛋白质鉴定。 通过使用CyDye的新型蛋白质分析方法包括以下步骤：（a）独立地将蛋白质标记用于分析，将蛋白质样品作为对照用不同的CyDye标记; （b）将50-100mug的步骤（a）中标记的每个蛋白质样品和1-5mg蛋白质进行二维凝胶电泳; （c）通过荧光分析分析电泳凝胶，分析蛋白质与蛋白质之间的差异; 和（d）通过使用MALDI-TOF从步骤（c）中分离的斑点分离和鉴定蛋白质。 此外，在步骤（c）中使用自动选择器进行荧光分析。