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公开(公告)号：KR100701209B1
公开(公告)日：2007-03-29
申请号：KR1020050082706
申请日：2005-09-06
Applicant: 충남대학교산학협력단
Abstract: 본 발명은 산업 효모인 야로이아 속 세포 표면에 목적단백질을 발현하기 위한 플라스미드 및 이를 이용한 목적단백질의 세포 표면 발현방법에 관한 것이다. 본 발명의 플라스미드는 세포질 내에 독립적으로 또는 크로모좀 내에 삽입되어 존재하며, 목적단백질을 야로이아 속 유래의 세포 표면 단백질과 융합하여 발현한다. 특히, 크로모좀에 삽입되어 존재하는 경우, 상응하는 크로모좀 상의 내인성 세포 표면 단백질을 코딩하는 유전자를 파쇄하여 목적단백질을 야로이아 리폴리티카 세포 표면에 보다 안정적으로 발현할 수 있다. 본 발명의 세포 표면 발현 시스템은 활성 단백질을 야로이아 속 세포 표면에 직접 발현함으로써 소수성 기질을 이용할 수 있는 야로이아 속의 효모를 세포촉매제로 직접 사용할 수 있는 유용성을 가진다.
야로이아 속 효모, 세포 표면 발현, 야로이아 리폴리티카, 세포벽 단백질.-
公开(公告)号：KR102161989B1
公开(公告)日：2020-10-06
申请号：KR1020190077706
申请日：2019-06-28
Applicant: 충남대학교산학협력단
IPC: H02K41/03 , F16F15/22 , F16F15/129
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Patent Agency Ranking
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公开(公告)号：KR102168401B1
公开(公告)日：2020-10-21
申请号：KR1020190077716
申请日：2019-06-28
Applicant: 충남대학교산학협력단
IPC: H02K41/03 , F16F15/22 , F16F15/129
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公开(公告)号：KR101401786B1
公开(公告)日：2014-05-30
申请号：KR1020120140839
申请日：2012-12-06
Applicant: 충남대학교산학협력단
IPC: G06K7/08
CPC classification number: G06K19/06187 , G06K19/06056
Abstract: The present invention relates to a magnetic tag capable of being recognized as a unique waveform by a magnetic pattern by forming a magnetic bit composed of the pattern of a magnetic material. The magnetic tag according to the present invention form the magnetic bit made from the magnetic material on one side of a bar-type body. The magnetic bit includes at least two magnetic patterns. Therefore, the magnetic tag minimizes interference between each magnetic bit, improves the accuracy of tag recognition, and facilitates high-capacity encoding in a fine range.
Abstract translation: 本发明涉及通过形成由磁性材料的图形构成的磁头，能够通过磁性图形识别为唯一波形的磁性标签。 根据本发明的磁性标签形成由杆状体的一侧上的磁性材料制成的磁头。 磁头包括至少两个磁性图案。 因此，磁标签使每个磁头之间的干扰最小化，提高了标签识别的准确性，并且有助于在精细范围内的高容量编码。
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公开(公告)号：KR101317047B1
公开(公告)日：2013-10-11
申请号：KR1020120080060
申请日：2012-07-23
Applicant: 충남대학교산학협력단
CPC classification number: G06K9/00315
Abstract: PURPOSE: An emotion recognition device using facial expressions and a control method are provided to measure the change for the facial expressions of an object, thereby objectively distinguishing the emotions which the object feels. CONSTITUTION: A camera (121) obtains a first image for a first object corresponding to emotions which are classified by a predetermined reference. A user input unit (130) receives a first frame of the first image from a user. A control unit (180) recognizes the face of the first object which is included in the first frame. The control unit extracts the facial component of the first object by using the face. The control unit extracts change patterns for the emotions by using the facial component. A memory (160) stores the change patterns for the emotions. [Reference numerals] (110) Wireless communication unit; (111) Broadcast reception module; (112) Mobile communication module; (113) Wireless internet module; (114) NFC module; (120) A/V input unit; (121) Camera; (122) Microphone; (130) User input unit; (140) Sensing unit; (141) Proximity sensor; (150) Output unit; (151) Display unit; (152) Sound output module; (153) Alarm unit; (154) Haptic module; (155) Projector module; (160) Memory; (170) Interface unit; (180) Control unit; (181) Multimedia module; (190) Power supply unit
Abstract translation: 目的：提供使用面部表情和控制方法的情感识别装置，以测量对象的面部表情的变化，从而客观地区分对象感觉到的情绪。 构成：照相机（121）获得对应于由预定参考分类的情绪的第一对象的第一图像。 用户输入单元（130）从用户接收第一图像的第一帧。 控制单元（180）识别包括在第一帧中的第一对象的面部。 控制单元使用面部提取第一物体的面部成分。 控制单元通过使用面部组件提取情绪的变化模式。 存储器（160）存储情绪的变化模式。 （附图标记）（110）无线通信单元; （111）广播接收模块; （112）移动通信模块; （113）无线上网模块; （114）NFC模块; （120）A / V输入单元; （121）相机; （122）麦克风; （130）用户输入单元; （140）感应单元; （141）接近传感器; （150）输出单元; （151）显示单元; （152）声音输出模块; （153）报警单元; （154）触觉模块; （155）投影机模块; （160）内存; （170）接口单元; （180）控制单元; （181）多媒体模块; （190）电源单元
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公开(公告)号：KR1020070027280A
公开(公告)日：2007-03-09
申请号：KR1020050082706
申请日：2005-09-06
Applicant: 충남대학교산학협력단
Abstract: A method for expressing a target protein using Yarrowia species yeast is provided to be able to express the target protein to a fused protein and a cell surface protein and express the target protein on the surface of a cell, and be able to more stably and efficiently induce the cell surface expression by introducing a plasmid into a chromosome of the yeast to crush the corresponding cell surface protein. The plasmid for cell surface expression comprises a cell surface protein encoding gene selected from the group consisting of YIAGA1, YICRH1, YICRH2, YICWP1, YIYWP1, YIPIR3 AND YISED1, a target protein encoding gene and a promoter operably linked to the upper portions thereof. The target protein encoding gene is fused into a cell surface protein during the expression in a cell to be expressed on the cell surface of Yarrowia species yeast such as Yarrowia lipolytica. The method for expressing a target protein on the cell surface of Yarrowia species yeast comprises the steps of: (a) transforming the plasmid into the Yarrowia species yeast; and (b) culturing the transformed plasmid to express the target protein on the surface of a host cell.
Abstract translation: 提供了使用耶氏酵母属酵母表达靶蛋白的方法，以能够将融合蛋白和细胞表面蛋白的靶蛋白表达，并在细胞表面表达靶蛋白，能够更稳定有效地 通过将质粒引入到酵母的染色体中以粉碎相应的细胞表面蛋白来诱导细胞表面表达。 用于细胞表面表达的质粒包含选自YIAGA1，YICRH1，YICRH2，YICWP1，YIYWP1，YIPIR3和YISED1的细胞表面蛋白质编码基因，靶蛋白编码基因和与其上部可操作地连接的启动子。 靶细胞编码基因在细胞表达期间融合到细胞表面蛋白质中，以在耶氏酵母属酵母例如解脂耶氏酵母（Yarrowia lipolytica）的细胞表面上表达。 在耶氏酵母菌细胞表面上表达靶蛋白的方法包括以下步骤：（a）将质粒转化为耶氏酵母属酵母; 和（b）培养转化的质粒以在宿主细胞的表面上表达靶蛋白。
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