Abstract:
PURPOSE: A SNP marker for predicting fatty acid composition of a crossbreed pig is provided to predict fatty acid composition, especially for a composition of unsaturated fatty acid and saturated fatty acid, thereby utilizing the SNP marker for selection of healthy pig entity and high value pork production. CONSTITUTION: A polynucleotide or corresponding polynucleotide for predicting fatty acid composition of a crossbreed pig is selected from polynucleotide having nucleic acid sequence of sequence number 1. The polynucleotide or corresponding polynucleotide comprises 0 or more of successive nucleic acids which include 817th of the sequence number 1 or 14384th SNP related base. The crossbreed pig is a crossbreed between Korea native kind and Landrace. The crossbreed pig is F_2. A polynucleotide which specifically mix with the polynucleotide or the corresponding polynucleotide is provided.
Abstract:
본 발명은, 이격 배치된 복수의 프리캐스트 콘크리트(Precast concrete) 기둥; 상기 복수의 프리캐스트 콘크리트 기둥 사이를 연결하는 복수의 보; 상기 각 프리캐스트 콘크리트 기둥의 상단부로부터 분기하여 수직 연장된 복수의 연결 브라켓; 및 상기 각 연결 브라켓과 상기 보를 결합시키는 결합수단을 포함하는 프리캐스트 콘크리트 기둥과 보의 연결 구조체를 제공한다. 본 발명은, 프리캐스트 방식으로 제작된 콘크리트 기둥과 보의 결합 시 케이블 부재를 개재하여 외부의 진동 시에는 구성 요소가 독립적으로 움직이고 진동 후에는 복원될 수 있도록 하고, 서로 결합되는 프리캐스트 콘크리트 기둥과 보의 결합면은 서로 대응하는 곡면으로 형성하여 기둥과 보의 진동 시 손상이 방지되도록 하는 효과가 있다.
Abstract:
PURPOSE: A post-installed anchor is provided to prevent an initial slip and largely obtain a pullout performance of an anchor. CONSTITUTION: A post-installed anchor (100) includes a pipe shaped sleeve (110), an anchor bolt (120), and a nut (130). The sleeve includes a cut piece (114) formed by a plurality of cutting grooves (112) on one end. The anchor bolt is inserted into the inside through one end of the sleeve. The anchor bolt includes a head unit on which a surface coming into contact with the end of the sleeve is slopingly formed. The length of the cutting groove is 1/3-1/5 of the sleeve length.
Abstract:
PURPOSE: A joint structure of precast concrete column and beam is provided to independently move components with vibration from the outside by interposing a cable member between the components and to restore the components after the vibration. CONSTITUTION: A joint structure of precast concrete column and beam comprises a plurality of precast concrete columns (110), beams (130), connection brackets (120), and joint units. A connection bracket is branched from the top end of a precast concrete column to be vertically extended. The joint unit includes a first through-hole inside a connection bracket, the second through-hole of a beam, and a cable member (150) joining a connection bracket to the beam by being inserted into the first and second through-holes. A column insertion part (140) is formed in a central part at the top end of a precast concrete column.
Abstract:
PURPOSE: A genetic marker for identifying fatty acid composition of Hanwoo(Korean beef) is provided to easily select Hanwoo with high quality. CONSTITUTION: A method for selecting Hanwoo with high unsaturated fatty acid content comprises: a step of isolating genome DNA sample from a Hanwoo; a step of amplifying PCR of a genetic marker of sequence number 1 containing SNP at 21th base using a primer; a step of cleaving a PCR product with restriction enzyme; a step of performing electrophoresis to identify fragment number and size; and a step of detecting genotype of 21th base. The primer is a primer of sequence numbers 3 and 4. The restriction enzyme is Rsal.
Abstract:
PURPOSE: A method for analyzing SNP of SCD(Stearoyl-CoA Desaturase) gene of a chicken is provided to produce and select high quality chickens, to improve farm income, and to increase sales. CONSTITUTION: A method for selecting a chicken with higher unsaturated fatty acid content than a chicken with heterozygous genotype at 2nd exon of chicken SCD gene comprises: a step of collecting a DNA sample from the chicken; a step of amplifying the DNA sample using the primer set of sequence numbers 1 and 2; a step of treating the amplified product with Acil; and a step of performing electrophoresis and identifying whether SNP of the 2nd exon of SCD is a homozygous genotype.
Abstract:
본 발명은 돼지 유전자에 삽입되어 있는 PERV의 연접서열 및 이를 이용한 PERV 검출용 프라이머 쌍에 관한 것으로서, 연접서열인 서열번호 1 내지 14 중 어느 하나를 포함하는 염기서열과, ① 상기 연접서열을 포함하는 염기서열에 상보적인 서열을 갖는 올리고 뉴클레오티드와, ② PERV 유전자 염기서열에 상보적인 서열을 갖는 올리고 뉴클레오티드로 이루어진 돼지 게놈 내 융합된 PERV 검출을 위한 프라이머 쌍에 관한 것이다. 본 발명에 의하면 돼지의 조직 또는 장기로부터 이종이식 시 문제의 소지가 있는 PERV의 존재를 사전에 검출할 수 있으며, 더 나아가 해당 유전자를 녹아웃(knock-out)시키는 데 이용할 수 있다. 돼지 내인성 레트로바이러스(PERV), 장기이식, 검출, 프라이머, 유전좌위, knock-out
Abstract:
A sequence of PERV (porcine endogenous retrovirus) and PERV primer pair using the same are provided to previously detect the presence of PERV and use for knock-out of a gene. A 3'-, or 5'-sequence to a porcine endogenous retrovirus (PERV) which is a sequence selected from the sequence numbers 1 to 14. A primer pair for detecting PERV comprises an oligonucleotide which has complementary sequence to the sequence numbers 1 to 14 and an oligonucleotide which has complementary sequence to the PERV gene sequence. The primer pair is used for detecting PERV through PCR (polymerase chain reaction) from porcine tissue or organ.